A comparison of two methods for determining titanium dioxide marker content in broiler digestibility studies

Morgan, Natalie K.; Scholey, Dawn; Burton, Emily

doi:10.1017/s1751731114000068

Cited by 21 publications

(13 citation statements)

References 10 publications

(22 reference statements)

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“…The ground digesta samples were analysed for titanium dioxide content by the method described in [ 29 ]. Apparent ileal zinc digestibility coefficients (COD) were obtained using the following equation (all measures in g/Kg Dry matter): (nutrient/TiO 2 ) diet − (nutrient/TiO 2 ) ileum digesta /(nutrient/TiO 2 ) diet …”

Section: Methodsmentioning

confidence: 99%

Bioavailability of Methionine-Coated Zinc Nanoparticles as a Dietary Supplement Leads to Improved Performance and Bone Strength in Broiler Chicken Production

Alkhtib

Scholey

Carter

et al. 2020

Animals

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Recently, nanotechnology has been widely adopted in many fields. The goal of this study was to evaluate the potential for amino acid coated nano minerals as a supplement in broiler feed. Zinc was selected as a model mineral for this test and supplementation of nano zinc, both coated and uncoated was compared with organic and inorganic commercial forms of zinc. A total of 48 pens (8 birds each) were assigned to one of the following dietary treatments: Control, methionine-Zinc chelate (M-Zn), nano zinc oxide (Nano-ZnO), and methionine coated nano zinc oxide (M-Nano-ZnO). All experimental diets were formulated with the same total zinc, methionine, protein, and energy content with just the zinc source as a variable. Bird weight, feed intake and feed conversion ratios were recorded weekly, with three birds culled (sacrificed) at day 21 and day 35 for sampling measures. Ileal digestibility of zinc was determined at day 21 and day 35 using titanium dioxide as an inert marker. Blood serum, liver and spleen samples were collected at day 21 and day 35 and analysed for zinc content via inductively coupled plasma mass spectrometry (ICP-MS). Tibia strength and morphometrics were measured from both legs of three birds per pen at day 21 and day 35. The study was conducted at Nottingham Trent University Poultry Unit, UK. The novel method of producing nano minerals coated with amino acids was successfully tested with zinc and material produced to test in the feeding study. Methionine coated nano zinc oxide supplementation significantly improved bird weight gain and the increased feed intake of broilers compared to an inorganic zinc form. Ileal digestibility was also improved with this methionine-nano zinc. Moreover, this supplementation improved the tibia strength of broilers at the age of 21 days, though this was not observed at day 35. Therefore, M-Nano-ZnO could be used to supplement broilers to improve both performance and digestibility with a limited positive impact on bone strength. The results of the current study suggest that the amino acid coating of nano minerals can improve the digestibility of minerals which may have further implications for the field of mineral nutrition in animal feeds.

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Section: Methodsmentioning

confidence: 99%

Bioavailability of Methionine-Coated Zinc Nanoparticles as a Dietary Supplement Leads to Improved Performance and Bone Strength in Broiler Chicken Production

Alkhtib

Scholey

Carter

et al. 2020

Animals

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“…Nitrogen content was analyzed by combustion (AOAC 1969) (Thermo Quest NA 2100 Nitrogen and Protein Analyzer; Breda, The Netherlands) and the crude protein ( CP ) content calculated using a 6.25 conversion factor. Titanium dioxide was determined by UV-spectroscopy as described before [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

Apparent ileal digestibility of Maillard reaction products in growing pigs

et al. 2018

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The absorption of Maillard reaction products (MRP) from dietary origin has been linked to the occurrence of chronic diseases. The aim of the present study was to determine the effects of toasting time of rapeseed meal (RSM) and the processing method of the diets (pelleting and extrusion) that included RSM on the apparent ileal digestibility (AID) of total lysine, fructosyl-lysine (FL), carboxymethyl-lysine (CML), carboxyethyl-lysine (CEL), lanthionine (LAN) and lysinoalanine (LAL) in growing pigs. The study consisted of a 2×3 factorial design with toasting time of RSM (60, 120 min) and diet processing method (mash, pelleted, extruded) as factors. Fifty growing pigs were individually fed one of the experimental diets for 4.5 consecutive days. Following euthanasia, samples of digesta were collected from the terminal 1.5 m of the small intestine. Increasing the toasting time of RSM increased the contents of FL, CML and CEL, whereas the additional effects of the diet processing methods were relatively small. Lysinoalanine and lanthionine were not detected in the diets; therefore, digestibility of these compounds could not be determined. The contents of FL, CML and CEL in the ileal chyme were positively correlated to their contents in the diets. The AID of the MRP from thermally-treated RSM were overall low and were not related to their contents in the diets. The AID of FL ranged between -8.5 and 19.1%, whilst AID of CML and CEL ranged from -0.2 to 18.3 and 3.6 to 30%, respectively. In conclusion, thermal treatments have clear effects on the contents of MRP in the diets. These compounds have relatively low digestibility in growing pigs.

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“…Phosphorus and Ca contents of the diets were analysed by inductively coupled plasma-optical emission spectroscopy (ICP-OES) following an aqua regia digestion step (AOAC 985.01, Leytem et al., 2008 ). Titanium dioxide was added at a rate of 0.5% to act as an inert marker for nutrient digestibility evaluation and the dietary content quantified by ICP-OES following aqua regia digestion ( Morgan et al., 2014 ). Total phytate content was analysed by a K-Phyt assay (Megazyme, Wicklow, Ireland, UK).…”

Section: Methodsmentioning

confidence: 99%

Effect of feeding broilers diets differing in susceptible phytate content

et al. 2016

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Measurements of total phytate phosphorus content of diets may be deceptive as they do not indicate substrate availability for phytase; it may be that measurements of phytate susceptible to phytase effects are a more accurate measure of phosphorus (P) availability to the bird. To verify this hypothesis, an experiment was conducted to compare diets formulated to contain either high or low susceptible phytate, supplemented with either 0 or 500 FTU/kg phytase. Susceptible phytate was determined by exposing the feed samples to conditions that mimicked the average pH of the proximal gastrointestinal tract (pH 4.5) and the optimum temperature for phytase activity (37 °C) and then measuring phytate dissolved. Ross 308 birds (n = 240) were fed one of 4 dietary treatments in a 2 × 2 factorial design; 2 diets with high (8.54 g/kg, 57.90% of total phytate) or low (5.77 g/kg, 46.33% of total phytate) susceptible phytate, containing 0 or 500 FTU/kg phytase. Diets were fed to broilers (12 replicate pens of 5 birds per pen) from d 0 to 28 post hatch. Birds fed diets high in susceptible phytate had greater phytate hydrolysis in the gizzard (P < 0.001), jejunum (P < 0.001) and ileum (P < 0.001) and resulting greater body weight gain (BWG) (P = 0.015) and lower FCR (P = 0.003) than birds fed the low susceptible phytate diets, irrespective of phytase presence. Birds fed the high susceptible diets also had greater P solubility in the gizzard and Ca and P solubility in the jejunum and ileum (P < 0.05) and resulting greater tibia and femur Ca and P (P < 0.05) content than those fed the low susceptible diets. All the susceptible phytate was fully degraded in the tract in the absence of added phytase, suggesting the assay used in this study was able to successfully estimate the amount of total dietary phytate that was susceptible to the effects of phytase when used at standard levels. No interactions were observed between susceptible phytate and phytase on phytate hydrolysis. Hydrolysis of phytate was greater (P < 0.05) in the gizzard of birds fed the diets supplemented with phytase, regardless of the concentration of susceptible phytate in the diet. Phytase supplementation resulted in improved BWG (P < 0.001) and FCR (P = 0.001), increased P solubility (P < 0.001) in the gizzard, Ca and P solubility (P < 0.001) in the jejunum and ileum and Ca and P concentration (P < 0.001) and strength (P < 0.001) in the tibia and femur. Pepsin activity was higher in birds fed the diets supplemented with phytase (P < 0.001) and was greater (P = 0.031) in birds fed the high susceptible phytate diets compared with the low susceptible phytate diets. Findings from this study suggest that there may be a measure more meaningful to animal nutritionists than measurements of total phytate.

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A comparison of two methods for determining titanium dioxide marker content in broiler digestibility studies

Cited by 21 publications

References 10 publications

Bioavailability of Methionine-Coated Zinc Nanoparticles as a Dietary Supplement Leads to Improved Performance and Bone Strength in Broiler Chicken Production

Bioavailability of Methionine-Coated Zinc Nanoparticles as a Dietary Supplement Leads to Improved Performance and Bone Strength in Broiler Chicken Production

Apparent ileal digestibility of Maillard reaction products in growing pigs

Effect of feeding broilers diets differing in susceptible phytate content

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