Resveratrol Reverses Cadmium Chloride-induced Testicular Damage and Subfertility by Downregulating p53 and Bax and Upregulating Gonadotropins and Bcl-2 gene Expression

Eleawa, Samy M; Alkhateeb, Mahmoud; Al‐Hashem, Fahaid; Bin-Jaliah, Ismaeel; Sakr, Hussein F.; El-Refaey, Hesham; El-Karib, Abbas O; Alessa, Riyad; Haidara, Mohamed A; Shatoor, Abdullah S.; Khalil, Mohammad

doi:10.1262/jrd.2013-097

Cited by 102 publications

(75 citation statements)

References 50 publications

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“…Resveratrol is known for its anti-inflammatory, antioxidant, analgesic, cardioprotective, anti-aging and neuroprotective roles (11–13). Studies have demonstrated that resveratrol may inhibit cell apoptosis, thereby providing protection from numerous diseases, including atherosclerosis, cerebral ischemia and myocardial ischemic reperfusion injury (14,15). Juan et al (16) reported that resveratrol is able to decrease germ cell apoptosis in mice and rats, and serves a protective role in the male reproductive tract, as well as enhancing blood testosterone levels, testicular sperm count and epididymis sperm motility in rabbits.…”

Section: Introductionmentioning

confidence: 99%

Protective effect of resveratrol on spermatozoa function in male infertility induced by excess weight and obesity

Cui¹,

Xuan

Wu³

et al. 2016

Molecular Medicine Reports

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Male infertility is a complex, multifactorial and polygenic disease that contributes to ~50% cases of infertility. Previous studies have demonstrated that excess weight and obesity factors serve an important role in the development of male infertility. An increasing number of studies have reported that resveratrol may regulate the response of cells to specific stimuli that induce cell injury, as well as decrease germ cell apoptosis in mice or rats. In the present study, the semen quality and serum sex hormone levels were evaluated in 324 men, which included 73 underweight, 82 normal weight, 95 overweight and 74 obese men. All patients were referred to The Reproductive Medicine Center of Shanxi Women and Infants Hospital (Taiyuan, China) between January 2013 and January 2015. The aim of the present study was to investigate the effects of resveratrol treatment on the motility, plasma zinc concentration and acrosin activity of sperm from obese males. The sperm concentration, normal sperm morphology, semen volumes, DNA fragmentation rates and testosterone levels in men from the overweight and obese groups were markedly decreased when compared with men in the normal weight group. In addition, the progressive motility, seminal plasma zinc concentration and spermatozoa acrosin activity were notably decreased in the obese group compared with the normal weight group. However, estradiol levels were significantly increased in the overweight, obese and underweight groups compared with the normal weight group. Notably, semen samples from obese males with astenospermia treated with 0–100 µmol/l resveratrol for 30 min demonstrated varying degrees of improvement in sperm motility. When these semen samples were treated with 30 µmol/l resveratrol, sperm motility improved when compared to other doses of resveratrol. Therefore, 30 µmol/l resveratrol was selected for further experiments. Upon treatment of semen samples with resveratrol (30 µmol/l) for 30 min, the seminal plasma zinc concentration and spermatozoa acrosin activity increased significantly in the experimental group compared with the control group. These data suggest that male obesity negatively impacts on male reproductive potential, not only through altering hormone levels, but also by directly altering sperm function. In addition, resveratrol may have a therapeutic and protective effect against obesity-induced abnormalities in semen.

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Section: Introductionmentioning

confidence: 99%

Protective effect of resveratrol on spermatozoa function in male infertility induced by excess weight and obesity

Cui¹,

Xuan

Wu³

et al. 2016

Molecular Medicine Reports

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“…Previous studies show that RES causes an increase in sperm count in healthy rats (Juan et al., ). Also, an increase in sperm count and motility was found in a study investigating the protective property of RES on testicular damage created in various ways (Elewa et al., ). Our study findings showed that RES treatment significantly protected sperm count and motility against EF damage.…”

Section: Discussionmentioning

confidence: 89%

The impact of electric fields on testis physiopathology, sperm parameters and DNA integrity-The role of resveratrol

et al. 2018

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This study investigated the long-term effects of electric fields (EF) which might cause physiopathological or morphological changes in the testis tissues of rats. We assumed that using resveratrol (RES) might reduce harmful effects of the EF. Thirty-two male Wistar Albino rats were randomly divided into four groups with eight animals in each; control, EF, EF + RES and RES. Malondialdehyde (MDA), superoxide dismutase, catalase, glutathione peroxidase and histopathological parameters were evaluated in testis tissue. Epididymal sperm count, motility and DNA damage were studied. Total testosterone, follicle-stimulating hormone, luteinising hormone, estradiol and growth hormone levels were evaluated in the plasma samples. EF caused statistically significant increase in MDA levels, body weight and DNA damage. A significant decrease was detected in sperm count and motility. The histopathological examination of the testes showed the germ cell decrease in the seminiferous epithelium with oedema and vascular congestion in the interstitial tissue. In immunohistochemical examination, the increase in the apoptotic cells number was detected. RES partially ameliorated biochemical, histopathological and immunohistochemical findings in the EF + RES group. These findings clearly demonstrated that EF can cause damage in rat testis. RES can ameliorate the damage caused by EF.

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“…RVT's antioxidant, antiinflammatory and antiapototic roles were studied in different experimental testis injury models such as ischemia/perfusion [39], cadmium chloride treatment [40] and polyvinyl chloridetreatment [41]. It was shown that RVT protects the Cis induced testis damage in mice by the inhibition of oxidative stress [42].…”

Section: Discussionmentioning

confidence: 99%

Resveratrol treatment reduces apoptosis and morphological alterations in cisplatin induced testis damage

Nagehan¹,

Şener²,

Ercan³

2019

jrp

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Cisplatin commonly used as a chemotheropotic agent however, it is associated with numerous side effects such as reproductive cytotoxicity. It causes spermatogenic cell death and DNA damage in spermatozoa via the formation of reactive oxygene species. Resveratrol (3,5,4'-trans-trihydroxystilbene), a natural phytoalexin, is a potent antioxidant agent, present in a wide variety of dietary sources including grapes, plums and peanuts. The aim of present study to evaluate the beneficial effects of resveratrol on cisplatin induced testis damage. Male Sprague Dawley rats were used in the study and four experimental groups were formed as: 1-saline applied control, 2-resveratrol applied control, 3-cisplatin and 4-cisplatin+resveratrol groups. Following a single dose of cisplatin (7 mg/kg i.p.), either saline or resveratrol (10 mg/kg, orally) was administered for 5 days. Testis samples were prepared for histopathological and ultrastructural evaluations, cell proliferation and apoptosis. Tissue malondialdehyde (MDA), glutathione (GSH) levels and myeloperoxidase (MPO) activity were determined biochemically. Degenerated and atrophic tubules of tissue, apoptotic cells, MDA level and MPO activity were increased although proliferation index and GSH level were decreased in cisplatin group. Degenerated tight junctions between the Sertoli cells and vacuole formation in germinal epithelial cells were also revealed at this group. However, resveratrol treatment reduced degenerated and atrophic tubules, apoptotic cells, vacuole formation in germinal epithelial cells, MDA level and MPO activity and increased proliferation index and GSH level in testis. These results showed that resveratrol ameliorates cisplatin induced testis injury by the impairment of oxidative stress and apoptosis.

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Resveratrol Reverses Cadmium Chloride-induced Testicular Damage and Subfertility by Downregulating p53 and Bax and Upregulating Gonadotropins and Bcl-2 gene Expression

Cited by 102 publications

References 50 publications

Protective effect of resveratrol on spermatozoa function in male infertility induced by excess weight and obesity

Protective effect of resveratrol on spermatozoa function in male infertility induced by excess weight and obesity

The impact of electric fields on testis physiopathology, sperm parameters and DNA integrity-The role of resveratrol

Resveratrol treatment reduces apoptosis and morphological alterations in cisplatin induced testis damage

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