Resveratrol treatment reduces apoptosis and morphological alterations in cisplatin induced testis damage

Abstract

Cisplatin commonly used as a chemotheropotic agent however, it is associated with numerous side effects such as reproductive cytotoxicity. It causes spermatogenic cell death and DNA damage in spermatozoa via the formation of reactive oxygene species. Resveratrol (3,5,4'-trans-trihydroxystilbene), a natural phytoalexin, is a potent antioxidant agent, present in a wide variety of dietary sources including grapes, plums and peanuts. The aim of present study to evaluate the beneficial effects of resveratrol on cisplatin induced testis damage. Male Sprague Dawley rats were used in the study and four experimental groups were formed as: 1-saline applied control, 2-resveratrol applied control, 3-cisplatin and 4-cisplatin+resveratrol groups. Following a single dose of cisplatin (7 mg/kg i.p.), either saline or resveratrol (10 mg/kg, orally) was administered for 5 days. Testis samples were prepared for histopathological and ultrastructural evaluations, cell proliferation and apoptosis. Tissue malondialdehyde (MDA), glutathione (GSH) levels and myeloperoxidase (MPO) activity were determined biochemically. Degenerated and atrophic tubules of tissue, apoptotic cells, MDA level and MPO activity were increased although proliferation index and GSH level were decreased in cisplatin group. Degenerated tight junctions between the Sertoli cells and vacuole formation in germinal epithelial cells were also revealed at this group. However, resveratrol treatment reduced degenerated and atrophic tubules, apoptotic cells, vacuole formation in germinal epithelial cells, MDA level and MPO activity and increased proliferation index and GSH level in testis. These results showed that resveratrol ameliorates cisplatin induced testis injury by the impairment of oxidative stress and apoptosis.

The protective role of resveratrol against sulfoxaflor‐induced toxicity in testis of adult male rats

“…Collodel et al 28 reported that RES showed an ameliorative effect against LPO and protected sperm from ROS attacks in sperm culture media mainly in mechanical techniques, such as semen cryopreservation, in which oxidative stress is exacerbated. Moreover, RES enhanced fertility and protected spermatogenesis by reducing oxidative stress and exerting anti‐apoptotic effects against testicular injury induced by many anticancer drugs 70–73 . In addition, it successfully increases the activity of the antioxidant defense system, prevents LPO, and improves the pathological changes in rats after malathion treatment 74 .…”

Regulation of radiation‐induced liver damage by modulation of SIRT‐1 activity: In vivo rat model

“…The rats were allocated into 4 groups at random, eight rats per group ( n = 8), assigned as follows: Group (1): Normal control; Group (2): Irradiated (IR) with 6 Gy 24 . Group (3): NAM + IR rats were dosed once daily at 100 mg/kg/dose 25 for 5 days before irradiation; Group (4): RSV + IR, rats were dosed once daily at 10 mg/kg/dose 26 for 5 days before irradiation.…”