Pemphigoid gestationis: light microscopic and direct immunofluorescense findings

Niflioğlu, Gülen Gül; Lebe, Banu

doi:10.5146/tjpath.2013.01172

Cited by 3 publications

(1 citation statement)

References 8 publications

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“…Images captured from 3 to 4 skin sections obtained from each animal were imaged at a magnification of 60×. The intensity of immunofluorescence within the epidermis was measured using ImageJ v1.8.0_112 (subtracting background fluorescence), and the mean immunofluorescence was determined for each mouse (8 mice per group) [51][52][53][54].…”

Section: Animal Modelmentioning

confidence: 99%

TLR3-Dependent Activation of TLR2 Endogenous Ligands via the MyD88 Signaling Pathway Augments the Innate Immune Response

Teixeira

Kazmierski

Kinane

et al. 2020

Cells

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The role of the adaptor molecule MyD88 is thought to be independent of Toll-like receptor 3 (TLR3) signaling. In this report, we demonstrate a previously unknown role of MyD88 in TLR3 signaling in inducing endogenous ligands of TLR2 to elicit innate immune responses. Of the various TLR ligands examined, the TLR3-specific ligand polyinosinic:polycytidylic acid (poly I:C), significantly induced TNF production and the upregulation of other TLR transcripts, in particular, TLR2. Accordingly, TLR3 stimulation also led to a significant upregulation of endogenous TLR2 ligands mainly, HMGB1 and Hsp60. By contrast, the silencing of TLR3 significantly downregulated MyD88 and TLR2 gene expression and pro-inflammatory IL1β, TNF, and IL8 secretion. The silencing of MyD88 similarly led to the downregulation of TLR2, IL1β, TNF and IL8, thus suggesting MyD88 to somehow act downstream of TLR3. Corroborating in vitro data, Myd88−/− knockout mice downregulated TNF, CXCL1; and phospho-p65 and phospho-IRF3 nuclear localization, upon poly I:C treatment in a mouse model of skin infection. Taken together, we identified a previously unknown role for MyD88 in the TLR3 signaling pathway, underlying the importance of TLRs and adapter protein interplay in modulating endogenous TLR ligands culminating in pro-inflammatory cytokine regulation.

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Section: Animal Modelmentioning

confidence: 99%

TLR3-Dependent Activation of TLR2 Endogenous Ligands via the MyD88 Signaling Pathway Augments the Innate Immune Response

Teixeira

Kazmierski

Kinane

et al. 2020

Cells

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Direct Immunofluorescence (Dif) Microscopy In Cutanous Small Vessel Vasculitis: A Single Institution Experiences

Oltulu¹,

Uğur²,

Özer³

et al. 2018

SDÜ Tıp Fakültesi Dergisi

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Küçük damar kutanöz vaskülitler inde (KKV) dir ekt immunfloresan mikroskopide (DİF) immun depolanmalar (özellikle IgG, IgM, IgA ve Kompleman C3) gözlenebilir. Dünya literatüründe çeşitli pozitiflik oranları bildiren çalışmalar mevcuttur. Çalışmamızda kliniğimize ait KKV'lerinin DİF mikroskopi sonuçlarını sunmayı amaçladık. Gereç ve yöntem: Vaskülit ön tanısı ile biopsi ve DİF tetkiki yapılan, histopatolojik olarak KKV'i mevcut olan toplam 121 olgu retrospektif olarak çalışmaya dahil edildi. Olgular klinik verileri ve Chapel Hill Consensus Conference vaskülit sınıflaması gözönünde bulundurularak toplam 6 gruba ayrıldı. Bazal membran ya da perivasküler (PV) alanda en az bir depolanma 'DİF pozitif' olarak kabul edildi. Tüm olgularda DİF IgG, IgM, IgA ve Compleman C3 depolanmalarının dağılımları, oranları ve gruplarda en az bir immun depozitin bulunma durumu belirlendi. Lökositoklazis bulunduran ya da eozinofil bulunduranlar ayrı grup yapılarak diğerleri ile immun depolanmalar açısından istatistiksel olarak karşılaştırıldı. Bulgular: Tüm olgularda DİF pozitifliği %58.7 (n:71/121) idi. Lökositoklastik vaskülit olgularının %50.9'unda (n:28/55), nonspesifik KV olgularının %67.4'ünde (n:31/46), ürtikeryal vaskülit olgularının %44.4 (n:4/9)'ünde, livedoid vaskülit olgularının %75 (n:3/4)'inde, henoch schonlein purpurası (HSP) olgularının (n:5/5) %100'ünde DİF pozitifti. 2 vaskülopati olgusunda depolanma yoktu. Lökositoklazis ve eozinofil mevcudiyeti ile immun depolanmalar arasında herhangi bir ilişki yoktu. En fazla biriken depozit C3 iken, HSP olgularında IgA depolanma oranı %100'dü. Sonuç: KKV'lerinde gözardı edilemeyecek yüksek DİF pozitiflik oranları (özellikle C3) tespit edildi. HSP olgularında DİF ile IgA depozit tespiti tanı için oldukça önemlidir. KKV'lerde DİF tetkikinin, klinik ve histopatolojik incelemeye ek olarak uygulanması faydalı olabilir.

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Histopathological Research and Diagnosis of Important Painting Methods

Gezen¹

2018

Health Sci. Q.

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Both diseases can safely in experimental research need to the histopathological diagnosis many coloring method utilized. This application of Immunohistochemistry and İmmunfloresan coloring methods stands out in terms of definitive diagnosis. Our country and other countries in which research scientists or Diagnostics these methods and which use antibodies to investigate the research planned and many Assembly-style research. İmmunfloresan coloring method, especially of some diseases affecting the immune system, resulting in the tissues of immunoglobulin and complement in the display of the Immunohistochemical staining method used to diagnose cancerous tissues. Both the painting cut early diagnosis of cancer in particular methods for specific investigations and used as safe.

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Pemphigoid gestationis: light microscopic and direct immunofluorescense findings

Cited by 3 publications

References 8 publications

TLR3-Dependent Activation of TLR2 Endogenous Ligands via the MyD88 Signaling Pathway Augments the Innate Immune Response

TLR3-Dependent Activation of TLR2 Endogenous Ligands via the MyD88 Signaling Pathway Augments the Innate Immune Response

Direct Immunofluorescence (Dif) Microscopy In Cutanous Small Vessel Vasculitis: A Single Institution Experiences

Histopathological Research and Diagnosis of Important Painting Methods

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