Histopathological Research and Diagnosis of Important Painting Methods

2018

Heavy metals, industrial and household wastes and pesticides are threats for the aquatic ecosystem. Polluted water sources are streaming into the seas and cause pollution in these systems. Dardanelles is exposed to pollution from the Marmara and Black Sea. Our previous studies demonstrated that the water and mollusc from certain regions of the Dardanelles contained heavy metal salts. The purpose of the study is to demonstrate the histopathologic changes in the gastric tissues of rats which are fed with great scallop (Pecten maximus) that are collected from the Çardak region of the Dardanelles. Four groups of rats are included in the study, group 1 (n=6), control group fed with standard rat food, group 2 (n=6), 75% great scallop and 25% standard rat food daily, group 3 (n=6), 75% great scallop and 25% standard rat food every two days, group 4 (n=6), 75% great scallop and 25% standard rat food every three days. After the routine histopathologic processing all gastric tissue samples are evaluated in terms of 8-hydroxy-2'-deoxyguanosine (8-OHdG) immunoreactivity with light microscopy and image analysis software. No histopathologic differences found in standard hematoxylin-eosin (H.E.) stained gastric tissue samples of the control group. Second group showed active chronic gastritis, third group showed less inflamation and chronic gastritis compared with the second group and fourth group showed less mononuclear inflammation compared to the second and third groups. In immunohistochemical staining, 8-OHdG immunoreactivity in gastric epithelial cells. 8-OHdG immunoreactivity was negative in stomach tissues in all groups. There was no statistically significant difference between the groups that were fed every day, every other day and every three days with great scallop (p>0.05). The results of our studies showed that rats fed more with great scallops could produce gastritis in the stomach.

Section: Discussionmentioning

confidence: 53%

HISTOPATHOLOGICAL CHANGES IN THE STOMACH MUCOSA OF RATS FED WITH GREAT SCALLOP (Pecten maximus)

Muratli

2018

“…In addition to the findings of other researchers, we have also found that heavy metal salts cause histopathological changes in the stomach. Gezen (2017) stated that immunohistochemical staining methods are used to detect damage to cells and tissues. The positive cells (8-OHdG) were predominantly observed in the areas of active inflammation with prominent cell infiltration (Seki et al 2002).…”

Section: Resultsmentioning

confidence: 99%

HISTOPATHOLOGICAL CHANGES IN THE STOMACH TISSUE OF RATS FED WITH MUSSELS (Mytilus galloprovincialis)

2018

Self Cite

Dardanelles is exposed to environmental pollution for many years. The strait is important in terms of fish migration and shellfish farming. Fish and shellfish obtained from the strait is frequently consumed by local people. Mussels serve as useful indicators of temporal trends in environmental quality because they accumulate some contaminants in their tissues at levels many time higher than in the surrrounding water and quickly to changes in contamination. Although there are few data about the toxicity of seafood that is exposed to environmental pollution, there are no animal studies about the gastric toxicity of mussels grown in the Dardanelles. Our previous studies demonstrated that the water and mollusc from certain regions of the Dardanelles contained heavy metal salts. The purpose of the study is to demonstrate the histopathologic changes in the gastric tissues of rats which are fed with mussels that are collected from the Çamburnu region of the Dardanelles. Four groups of rats are included in the study, group 1 (n=6), control group fed with standard rat food, group 2 (n=6), 75% mussels and 25% standard rat food daily, group 3 (n=6), 75% mussels and 25% standard rat food every two days, group 4 (n=6), 75% mussels and 25% standard rat food every three days. After the routine histopathologic processing all gastric tissue samples are evaluated in terms of 8-hydroxy-2'-deoxyguanosine (8-OHdG) immunoreactivity with light microscopy and image analysis software. No histopathologic differences found in standard hematoxylin-eosin (H.E.) stained gastric tissue samples of the control group. In the second and third groups, a small amount of mononuclear cell infiltration was detected. In the fourth group, no significant difference was observed in mononuclear cells. In immunohistochemical staining, 8-OHdG immunoreactivity in gastric epithelial cells. 8-OHdG immunoreactivity was negative in gastric tissues in all groups. There was no statistically significant difference between the groups that were fed every day, every other day and every three days with clam (p>0.05).

“…Some researchers have found that heavy metal salts can cause DNA damage (Fraga et al 1990;Halliwell, 2000;Williams et al 2000;Ateş et al 2004;Burçak et al 2004;Siomek et al 2006). Immunohistochemical staining methods are used to detect cell and tissue damage (Gezen, 2017). In this study, we investigated whether or not to produce DNA damage by feeding the carpet shell clam containing heavy metal salts to rats.…”

Section: Discussionmentioning

confidence: 96%

HISTOPATHOLOGICAL CHANGES IN THE STOMACH MUCOSA OF RATS FED WITH CARPET SHELL CLAM (Ruditapes decussatus)

Muratli

2018

Free radicals are produced in cells by cellular metabolism and exogenous agents. These specie reacts with biomolecules in cells, including DNA, proteins, lipids and carbonhydrates. The resulting oxidative damage to DNA, is implicated in mutagenesis, carcinogenesis, and aging. Heavy metals, industrial and household wastes and pesticides are threats for the aquatic ecosystem. Polluted water sources are streaming into the seas and cause pollution in these systems. Dardanelles is exposed to pollution from the Marmara and Black Sea. Our previous studies demonstrated that the water and mollusc from certain regions of the Dardanelles contained heavy metal salts. The purpose of the study is to demonstrate DNA damage and the histopathologic changes in the gastric tissues of rats which are fed with carpet shell clam that are collected from the Çardak Lagoon (Çanakkale, Turkey). Four groups of rats are included in the study, group 1 (n=6), control group fed with standard rat food, group 2 (n=6), 75% carpet shell clam and 25% standard rat food daily, group 3 (n=6), 75% carpet shell clam and 25% standard rat food every two days, group 4 (n=6), 75% carpet shell clam and 25% standard rat food every three days. After the routine histopathologic processing all gastric tissue samples are evaluated in terms of 8hydroxy-2'-deoxyguanosine (8-OHdG) immunoreactivity with light microscopy and image analysis software. No histopathologic differences found in standard hematoxylin-eosin stained gastric tissue samples of the control group. Mucosal specimens of the second, third and fourth groups showed mild mononuclear inflammation in favor of chronic gastritis. In immunohistochemical staining, 8-OHdG immunoreactivity in gastric epithelial cells. 8-OHdG immunoreactivity was negative in stomach tissues in all groups. There was no statistically significant difference between the groups that were fed every day, every other day and every three days with clam (p>0.05). The results of our studies showed that rats fed more with carpet shell clam could produce gastritis in the stomach.