Application of Online Preconcentration Affinity Capillary Electrophoresis Method to Glycans Labeled with 8-Aminonaphthalene-1,3,6-trisulfonic Acid Using Blue Light Emitting Diode-induced Fluorescence Detection

Yamamoto, Sachio; Nakatani, Yumi; Suzuki, Shigeo

doi:10.2116/analsci.29.831

Cited by 9 publications

(4 citation statements)

References 26 publications

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“…The high specificity of lectin binding to oligosaccharides is helpful for glycoproteomic profiling analysis. Lectin affinity capillary electrophoresis [48][49][50] has been utilized for this purpose.…”

Section: Affinity Me Of Oligosaccharides Utilizing Lectin-immobilized...mentioning

confidence: 99%

<i>In Situ </i>Photopolymerization of Functionalized Polyacrylamide-Based Preconcentrators for Highly Sensitive Specific Detection of Various Analytes by Microchip Electrophoresis

Yamamoto

2021

Chromatography

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Microchip electrophoresis (ME) has emerged as a promising tool for rapid analysis of various sample types and it is one of the most important modules of miniaturized total analysis systems. Electrokinetic effects provide both efficient transport and separation of sample components in microfluidic channels. Moreover, miniaturization of devices drastically reduces reagent consumption, significantly decreases analysis time, and allows for easier parallel screening and automation. However, most microfluidic systems are insensitive, which is a serious problem. In order to increase the sensitivity of ME, several on-line pretreatment applications such as specific and non-specific preconcentration, extraction, and derivatization have been proposed. Here, we review various specific on-line preconcentration methods utilizing in situ photopolymerized polyacrylamide-based preconcentrator gels. These techniques allowed for highly sensitive specific detection of various sample types by ME.

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Section: Affinity Me Of Oligosaccharides Utilizing Lectin-immobilized...mentioning

confidence: 99%

<i>In Situ </i>Photopolymerization of Functionalized Polyacrylamide-Based Preconcentrators for Highly Sensitive Specific Detection of Various Analytes by Microchip Electrophoresis

Yamamoto

2021

Chromatography

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“…LVSEP was recently used for a range of applications including parabens in cosmetics , selenomethionine enantiomers in selenized yeast after off‐line SPE , glucose ladder, glycoprotein‐derived oligosaccharides in plant lectin ; benzylamine and 1‐naphthylethylamine, chlorpheniramine, brompheniramine, and basic proteins (cytochrome c, ribonuclease A, lysozyme, and alpha‐chymotrypsinogen A) ; racemic warfarin and ibuprofen for chiral analysis ; ima‐amine, N ‐desmethyl matinib mesylate, and related compounds in capsule drugs ; isbenzylisoquinoline alkaloids ; kynurenine and tryptophan in human plasma used for biomarkers for immune systems ; and sulfonylurea herbicides in cereals .…”

Section: Stackingmentioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2012–2014)

et al. 2015

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One of the most cited limitations of capillary (and microchip) electrophoresis is the poor sensitivity. This review continues to update this series of biannual reviews, first published in Electrophoresis in 2007, on developments in the field of on-line/in-line concentration methods, covering the period July 2012-July 2014. It includes developments in the field of stacking, covering all methods from field-amplified sample stacking and large-volume sample stacking, through to ITP, dynamic pH junction, and sweeping. Attention is also given to on-line or in-line extraction methods that have been used for electrophoresis.

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“… and Yamamoto et al. , LVSS with EOF pumping was combined with partial filling affinity CE to create a highly sensitive method for studying the interaction of glycoprotein‐derived oligosaccharides with plant lectins. Oligosaccharides were derivatized with 8‐aminonaphthalene‐1,3,6‐trisulfonic acid for blue light emitting diode induced fluorescence detection.…”

Section: Kohlrausch Adjustment Of Concentrationmentioning

confidence: 99%

Contemporary sample stacking in analytical electrophoresis

et al. 2014

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This contribution is a methodological review of the publications about the topic from the last 2 years. Therefore, it is primarily organized according to the methods and procedures used in surveyed papers and the origin and type of sample and specification of analytes form the secondary structure. The introductory part about navigation in the architecture of stacking brings a brief characterization of the various stacking methods, with the description of mutual links to each other and important differences among them. The main body of the article brings a survey of publications organized according to main principles of stacking and then according to the origin and type of the sample. Provided that the paper cited gave explicitly the relevant data, information about the BGE(s) used, procedure, detector employed, and reached LOD and/or concentration effect is given. The papers where the procedure used is a combination of diverse fragments and parts of various stacking techniques are mentioned in a special section on combined techniques. The concluding remarks in the final part of the review evaluate present state of art and the trends of sample stacking in CE.

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Application of Online Preconcentration Affinity Capillary Electrophoresis Method to Glycans Labeled with 8-Aminonaphthalene-1,3,6-trisulfonic Acid Using Blue Light Emitting Diode-induced Fluorescence Detection

Cited by 9 publications

References 26 publications

<i>In Situ </i>Photopolymerization of Functionalized Polyacrylamide-Based Preconcentrators for Highly Sensitive Specific Detection of Various Analytes by Microchip Electrophoresis

<i>In Situ </i>Photopolymerization of Functionalized Polyacrylamide-Based Preconcentrators for Highly Sensitive Specific Detection of Various Analytes by Microchip Electrophoresis

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2012–2014)

Contemporary sample stacking in analytical electrophoresis

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