Effect of autologous stem cells on regenerated bone during distraction osteogenesis by Ilizarov technique in the radius of dogs: histomorphometric analysis

Abstract

PURPOSE:To investigate by histomorphometry the distraction osteogenesis by Ilizarov technique in dog radius with the use of autologous stem cells in regenerated bone. METHODS:Ten dogs (20 radiuses) underwent the osteotomy of 20% of extension of their radiuses, and osteogenic distraction using the Ilizarov technique after this procedure at rate of 1mm per day divided into 0.5mm every 12 hours. The elongation was performed until the regeneration reached 20% of the total length of the radius. The stem cells were isolated, concentrated and injected in the regenerated bone, when it reached 10% of the length of the entire radius. The regenerated bone was evaluated using histomorphometric analysis when the elongation was 20% the size of radius. RESULTS:The bone formation was evidenced by histomorphometric indices were significantly greater in the study group. In the histology evaluation the type of healing was mixed in 80% (intra membrane and endocondral) in both groups; the osteoblastic activity from moderate to intense was greater in the study group; and the space occupied by the newly-formed bone tissue was more evident in the study group. CONCLUSIONS:The histomorphometric indices in this study expressing the microarchitecture, trabecular thickness, trabecular spacing, number of trabecula and quantity of bone that was significant in the group study. These data suggest that the use of undifferentiated stem cells autologous bone marrow in the regenerate bone induces osteogenesis and bone quality.

Behaviour of adipose-derived canine mesenchymal stem cells after superparamagnetic iron oxide nanoparticles labelling for magnetic resonance imaging

“…The use of stem cells is becoming increasingly important in veterinary medicine. Mesenchymal stem cells (MSCs) have been shown to improve tissue repair in oral ulcers [ 1 , 2 ] and bone defects [ 3 – 6 ], as well as in dogs with osteoarthritis of the coxofemoral and elbow joint [ 7 – 10 ]. MSCs have also been used in canine central nervous system to treat spinal cord injury [ 11 – 14 ] and ischemic brain infarction [ 15 ].…”

The effect of femoral nerve block on fracture healing via expressions of growth factors and β-catenin

“…Five serial sections from each leg and a minimum of five adjacent fields in each section were quantified at a magnification of ×40 objective lens. The thicknesses of the periosteum, number, thickness, and areas of trabeculae and cartilaginous new bone were evaluated histomorphometrically with a use of semiautomatic image analysis system (University of Texas Health Science Center at Santorino, TX, USA image tool for Windows version 1.28 program) as described by other authors [6,[21][22][23][24]. All of the measurements were made in a blinded manner by a histologist.…”