Behaviour of adipose-derived canine mesenchymal stem cells after superparamagnetic iron oxide nanoparticles labelling for magnetic resonance imaging

Kolecka, Malgorzata; Arnhold, Stefan; Schmidt, Martin J.; Reich, Christine Maria; Kramer, Martin; Failing, Klaus; Pückler, Kerstin von

doi:10.1186/s12917-017-0980-0

Cited by 14 publications

(22 citation statements)

References 37 publications

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“…Previous studies have shown that labelling cells with iron oxide nanoparticles can inhibit differentiation to specific lineages [22,23]. In our study, we did not find any evidence that the bicistronic Fluc-ZsGreen reporter or the MPIOs inhibited the differentiation of hESC-derived DAPCs into TH + DA neurons.…”

Section: Discussioncontrasting

confidence: 81%

Assessing Human Embryonic Stem Cell-Derived Dopaminergic Neuron Progenitor Transplants Using Non-invasive Imaging Techniques

et al. 2020

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Purpose Human pluripotent stem cell (hPSC)-derived dopaminergic neuron progenitor cells (DAPCs) are a potential therapy for Parkinson’s disease (PD). However, their intracranial administration raises safety concerns including uncontrolled proliferation, migration and inflammation. Here, we apply a bimodal imaging approach to investigate the fate of DAPC transplants in the rat striatum. Procedures DAPCs co-expressing luciferase and ZsGreen or labelled with micron-sized particles of iron oxide (MPIOs) were transplanted in the striatum of RNU rats (n = 6 per group). DAPCs were tracked in vivo using bioluminescence and magnetic resonance (MR) imaging modalities. Results Transgene silencing in differentiating DAPCs accompanied with signal attenuation due to animal growth rendered the bioluminescence undetectable by week 2 post intrastriatal transplantation. However, MR imaging of MPIO-labelled DAPCs showed that transplanted cells remained at the site of injection for over 120 days. Post-mortem histological analysis of DAPC transplants demonstrated that labelling with either luciferase/ZsGreen or MPIOs did not affect the ability of cells to differentiate into mature dopaminergic neurons. Importantly, labelled cells did not elicit increased glial reactivity compared to non-labelled cells. Conclusions In summary, our findings support the transplantation of hPSC-derived DAPCs as a safe treatment for PD.

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Section: Discussioncontrasting

confidence: 81%

Assessing Human Embryonic Stem Cell-Derived Dopaminergic Neuron Progenitor Transplants Using Non-invasive Imaging Techniques

et al. 2020

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“…Canine adipose-derived stem cells were incubated with superparamagnetic iron oxide nanoparticles (SPIO: 319.2 μg/mL Fe) for 24 h. The iron oxide nanoparticles enter into the cells via endocytosis. Further the results showed there is no significant harmful effect on the viability of adipose-derived canine mesenchymal stem cells; therefore, it can be used for as contrast agent [ 156 ]. Long et al reported that ultra-small superparamagnetic iron oxide nanoparticles used as MRI tracking agent to measure the seizure disorders in a rat model of temporal lobe epilepsy [ 157 ].…”

Section: Types Of Various Nanomaterials Used In Veterinary Medicinmentioning

confidence: 99%

Theranostics Aspects of Various Nanoparticles in Veterinary Medicine

Bai

Lin

Huang

et al. 2018

IJMS

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Nanoscience and nanotechnology shows immense interest in various areas of research and applications, including biotechnology, biomedical sciences, nanomedicine, and veterinary medicine. Studies and application of nanotechnology was explored very extensively in the human medical field and also studies undertaken in rodents extensively, still either studies or applications in veterinary medicine is not up to the level when compared to applications to human beings. The application in veterinary medicine and animal production is still relatively innovative. Recently, in the era of health care technologies, Veterinary Medicine also entered into a new phase and incredible transformations. Nanotechnology has tremendous and potential influence not only the way we live, but also on the way that we practice veterinary medicine and increase the safety of domestic animals, production, and income to the farmers through use of nanomaterials. The current status and advancements of nanotechnology is being used to enhance the animal growth promotion, and production. To achieve these, nanoparticles are used as alternative antimicrobial agents to overcome the usage alarming rate of antibiotics, detection of pathogenic bacteria, and also nanoparticles being used as drug delivery agents as new drug and vaccine candidates with improved characteristics and performance, diagnostic, therapeutic, feed additive, nutrient delivery, biocidal agents, reproductive aids, and finally to increase the quality of food using various kinds of functionalized nanoparticles, such as liposomes, polymeric nanoparticles, dendrimers, micellar nanoparticles, and metal nanoparticles. It seems that nanotechnology is ideal for veterinary applications in terms of cost and the availability of resources. The main focus of this review is describes some of the important current and future principal aspects of involvement of nanotechnology in Veterinary Medicine. However, we are not intended to cover the entire scenario of Veterinary Medicine, despite this review is to provide a glimpse at potential important targets of nanotechnology in the field of Veterinary Medicine. Considering the strong potential of the interaction between the nanotechnology and Veterinary Medicine, the aim of this review is to provide a concise description of the advances of nanotechnology in Veterinary Medicine, in terms of their potential application of various kinds of nanoparticles, secondly we discussed role of nanomaterials in animal health and production, and finally we discussed conclusion and future perspectives of nanotechnology in veterinary medicine.

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“…(2) PLGA/IO MPs (PLL) was able to label TSCs and these labeled cells could be noninvasively monitored by both MRI and PA imaging for 21 days and 7 days respectively in rat rotator cuff injury model; (3) The therapeutic capacity of TSCs was not affected by PLGA/IO MPs labeling in a rat model of rotator cuff injury. As we known, iron oxide nanoparticles (IO NPs) like superparamagnetic iron oxide (SPIO) have been extensively used as contrast agents for MR tracking because of their favorable biocompatibility and the high sensitivity in T2*-weighted images 17,[28][29][30][31] . The sensitivity is dependent on the IO NPs loading of the cell as well as the density of labeled cells in an imaging voxel 32 .…”

Section: Discussionmentioning

confidence: 99%

“…As we known, iron oxide nanoparticles (IO NPs) like superparamagnetic iron oxide (SPIO) have been extensively used as contrast agents for MR tracking because of their favorable biocompatibility and the high sensitivity in T2*-weighted images 17 , 28 – 31 . The sensitivity is dependent on the IO NPs loading of the cell as well as the density of labeled cells in an imaging voxel 32 .…”

Section: Discussionmentioning

confidence: 99%

Dual-modal magnetic resonance and photoacoustic tracking and outcome of transplanted tendon stem cells in the rat rotator cuff injury model

Cheng

et al. 2020

Sci Rep

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Stem cells have been used to promote the repair of rotator cuff injury, but their fate after transplantation is not clear. Therefore, contrast agents with good biocompatibility for labeling cell and a reliable technique to track cell are necessary. Here, we developed a micron-sized PLGA/IO MPs to label tendon stem cells (TSCs) and demonstrated that PLGA/IO MPs were safe and efficient for longterm tracking of TSCs by using dual-modal MR and Photoacoustic (PA) imaging both in vitro and in rat rotator cuff injury. Moreover, TSCs improved the repair of injury and the therapeutic effect was not affected by PLGA/IO MPs labeling. We concluded that PLGA/IO particle was a promising dual-modal MR/PA contrast for noninvasive long-term stem cell tracking. Rotator cuff injury is one of the most common shoulder diseases, which occur most often in people who repeatedly perform overhead motions in their jobs or sports 1. The conventional therapies used for rotator cuff injury include analgesics, anti-inflammatory drugs, physiotherapy, steroids injection, and surgical repair 2. In fact, conservative treatments usually show short-term pain relief but lack long-term efficacy 3. Despite advances in surgical treatment options, the failure rates of rotator cuff repairs were still as high as 20% 4. In recent years, stem-cell-driven regeneration is gaining increased attention, and mesenchymal stromal cells (MSCs) have been widely applied for the treatment of rotator cuff tears on the basis of their self-renewal, clonogenicity, and multidifferentiation potential 5-12. But Mohammad et al. found that intra-synovial implantation of marrow-derived MSCs did not promote tendon healing in ovine deep digital flexor tendon injury model 12. While, tendon stem cells (TSCs) display high clonogenecity, cell proliferation, and tenogenic-differentiation potential compared to bone marrow mesenchymal stromal cells since Bi et.al firstly identified them in 2007, suggesting that they could be a better cell source for tendon regeneration 13. To develop effective TSCs therapies, it is imperative to label and track the administrated cells, preferably in a noninvasive manner with high efficiency. Recently, optical imaging, radionuclide imaging, magnetic resonance imaging (MRI), ultrasound (US), photoacoustic (PA) imaging and computed tomography (CT), have been widely used for cell tracking 14-19. However, each individual imaging has its own advantages and limitations. Optical imaging suffers from shallow penetration depth and phototoxicity, and radionuclide imaging has poor spatial resolution and rapid decay of radioisotopes 20,21. MRI, as the gold standard stem cell tracking modality, has high spatial resolution and deep penetration but has a relatively poor temporal resolution 19,22. While, PA have excellent temporal resolution but suffer from poor penetration depth 16,23. Therefore, nanoparticles based dual-/multi-modal imaging modalities that allow long-term tracking of stem cells with nontoxicity and good biocompatibility are need 24,25 .

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Behaviour of adipose-derived canine mesenchymal stem cells after superparamagnetic iron oxide nanoparticles labelling for magnetic resonance imaging

Cited by 14 publications

References 37 publications

Assessing Human Embryonic Stem Cell-Derived Dopaminergic Neuron Progenitor Transplants Using Non-invasive Imaging Techniques

Assessing Human Embryonic Stem Cell-Derived Dopaminergic Neuron Progenitor Transplants Using Non-invasive Imaging Techniques

Theranostics Aspects of Various Nanoparticles in Veterinary Medicine

Dual-modal magnetic resonance and photoacoustic tracking and outcome of transplanted tendon stem cells in the rat rotator cuff injury model

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