In Vitro Antimicrobial Activity of Plant-Derived Diterpenes against Bovine Mastitis Bacteria

Abstract

Abstract:We evaluated the antibacterial activity of three diterpenes isolated from natural sources against a panel of microorganisms responsible for bovine mastitis. ent-Copalic acid (CA) was the most active metabolite, with promising MIC values (from 1.56 to 6.25 µg mL −1 ) against Staphylococcus aureus (ATCC and clinical isolate), Staphylococcus epidermidis, Streptococcus agalactiae, and Streptococcus dysgalactiae. We conducted time-kill assays of CA against S. aureus, a commensal organism considered to be a ubiquitous etiological agent of bovine mastitis in dairy farms worldwide. In the first 12 h, CA only inhibited the growth of the inoculums (bacteriostatic effect), but its bactericidal effect was clearly noted thereafter (between 12 and 24 h). In conclusion, CA should be considered for the control of several Gram-positive bacteria related to bovine mastitis.

Antibacterial compounds from Salvia adenophora Fernald (Lamiaceae)

“…Diterpenes are reported to display a wide spectrum of biological activities, including antibacterial action (Almeida et al, 2008;Kuzma et al, 2007;Porto et al, 2009aPorto et al, , 2009b. Diterpenoids, and particularly clerodanes, isolated from species of Salvia were also shown to possess antimicrobial activity (Fonseca et al, 2013;Kuzma et al, 2007;Radulovic et al, 2010;Stavri et al, 2009;Ulubelen, 2003;Wu et al, 2012). Our results confirm the contention that clerodanes and especially the group of oxylipins, can be considered as a source of new and interesting antimicrobial agents that deserve further study.…”

Control of Bovine Mastitis: Old and Recent Therapeutic Approaches

“…Entcopalic acid (CA) was the most active against most of the microorganisms tested. Besides its antibacterial potential, CA did not to cause a cytotoxic effect on human fibroblast cell line, so this fact encourages its possible use on bovine mastitis control and treatment [19]. Baskaran et al [6] also investigated the antimicrobial activity of plant-derived molecules on a wide array of bacterial mastitis pathogens in milk, namely, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis, S. aureus, and E. coli.…”

Composição química e eficácia do óleo essencial e do extrato etanólico de Alpinia zerumbet sobre Staphylococcus aureus

“…O aparecimento de cepas multirresistentes é favorecido pelo uso indiscriminado de antimicrobianos que, quando utilizados em animais de produção, pode levar ao acúmulo de resíduos nos alimentos, com sérias consequências para a saúde do consumidor (Mota et al, 2005). Portanto, faz-se necessária a descoberta de compostos novos e seguros para controle da mastite bovina (Fonseca et al, 2013).…”