β‐Carotene, α‐tocopherol and ascorbic acid: differential profile of antioxidant, inflammatory status and regulation of gene expression in human mononuclear cells of diabetic donors

Oliveira, Braulio; Costa, Daniela Caldeira; Nogueira-Machado, José Augusto; Chaves, Mı́riam Martins

doi:10.1002/dmrr.2439

Cited by 15 publications

(17 citation statements)

References 40 publications

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“…Also relevant to ROS and glioma risk is the inverse association we observed for alpha-tocopherol, the most biologically active form of vitamin E and a potent inhibitor of lipid peroxidation [ 36 ]. Two previous studies had similar findings for glioma and glioblastoma [ 37 , 38 ], while a recent report showed positive associations for several antioxidants including alpha-tocopherol [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

A prospective study of serum metabolites and glioma risk

et al. 2017

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Malignant glioma is one of the most lethal adult cancers, yet its etiology remains largely unknown. We conducted a prospective serum metabolomic analysis of glioma based on 64 cases and 64 matched controls selected from Alpha-Tocopherol, Beta-Carotene Cancer Prevention (ATBC) Study. Median time from collection of baseline fasting serum to diagnosis was nine years (inter-decile range 3-20 years). LC/MS-MS identified 730 known metabolites, and conditional logistic regression models estimated odds ratios for one-standard deviation differences in log-metabolite signals. Forty-three metabolites were associated with glioma at P<0.05. 2-Oxoarginine, cysteine, alpha-ketoglutarate, chenodeoxycholate and argininate yielded the strongest metabolite signals and were inversely related to overall glioma risk (0.0065≤P<0.0083). Also, seven xanthine metabolites related to caffeine metabolism were higher in cases than in controls (0.017≤P<0.042). Findings were mostly similar in high-grade glioma cases, although prominent inversely associated metabolites included the secondary bile acids glycocholenate sulfate and 3β-hydroxy-5-cholenoic acid, xenobiotic methyl 4-hydroxybenzoate sulfate, sex steroid 5alpha-pregnan-3beta, 20beta-diol-monosulfate, and cofactor/vitamin oxalate (0.0091≤P<0.021). A serum metabolomic profile of glioma identified years in advance of clinical diagnoses is characterized by altered signals in arginine/proline, antioxidant, and coffee-related metabolites. The observed pattern provides new potential leads regarding the molecular basis relevant to etiologic or sub-clinical biomarkers for glioma.

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Section: Discussionmentioning

confidence: 99%

A prospective study of serum metabolites and glioma risk

et al. 2017

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“…Hyperglycemia induces an increase in intracellular ROS generation, and activates intracellular metabolic pathways, such as the polyol pathway, and the PKC signaling leading to suppression of antioxidant systems enzymes (e.g., catalase, glutathione peroxidase) and compounds (e.g., vitamins C and E) . Hyperglycemia also increases secretion of pro‐inflammatory cytokines and growth factors, impairs the immune cells functions, leads to DNA damage, increases protein glycation and induces generation of lipid peroxidation products, such as lipid hydroperoxides and malondialdehyde . The imbalance between production of pro‐oxidant compounds and the antioxidant defense system results in oxidative stress, which damages cell structures and plays a negative role in cellular events that are critically important for wound healing such as the resolution of inflammation, increased cellular senescence and apoptosis …”

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confidence: 99%

“…Administration of antioxidant agents such as vitamins E and C, β‐carotene, lipoic acid, N ‐acetylcysteine (associated or isolated) have been reported to prevent the deleterious effects of hyperglycemia on cytokine expression, cell growth and altered extracellular matrix homeostasis in culture, in animal models of diabetes and in diabetic patients . Treatment with vitamins E or C, however, generated conflicting results, mostly due to their potential pro‐oxidant effects . Nonetheless, these vitamins ameliorate some of the diabetic complications such as nephropathy, retinopathy, endothelial dysfunction and others …”

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confidence: 99%

Oral administration of antioxidants improves skin wound healing in diabetic mice

Pessoa

Florim

Rodrigues

et al. 2016

Wound Repair Regeneration

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Oxidative stress aggravates several long-term complications in diabetes mellitus. We evaluated the effectiveness of the oral administration of antioxidants (vitamins E and C, 40 and 100 mg/kg b.w., respectively) on skin wound healing acceleration in alloxan-induced diabetic mice. Mice were wounded 30 days after the induction of diabetes. Antioxidants were effective in preventing oxidative stress, as assessed by TBARS. The enzymes catalase, glutathione reductase, glutathione peroxidase, and superoxide dismutase were increased in diabetics on the 3rd day post-wounding; catalase and glutathione peroxidase remained still augmented in diabetics after 14th day postwounding, and the treatment with vitamins restored their activities to control. After 3 days, diabetic mice showed lower infiltration of inflammatory cells (including CD11b and Ly6G cells) and reduced levels of KC, TNF-α, IL-1β, and IL-12 p40 when compared with control mice. The treatment restored cytokine levels. After 14 days, diabetic mice showed late wound closure, persistent inflammation and delayed reepithelialization, accompanied by an increase in MIG /CD206 macrophages whereas CD206 /MIG macrophages were decreased. Cytokines IL-12p40, TNF-α, IL-1β, and KC were increased and normal levels were restored after treatment with antioxidants. These results suggest that oxidative stress plays a major role in diabetic wound healing impairment and the oral administration of antioxidants improves healing by modulating inflammation and the antioxidant system with no effect on glycemia.

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“…A recent research tracing several biochemical and molecular markers of oxidative damage and antioxidative defense reported dual effects of vitamin C and vitamin E combination, pro-oxidant and antioxidant, depending on the doses and other experimental conditions (de Oliveira et al, 2013). Therefore, the future research with more detailed and focused designs, using various laboratory assays and different experimental conditions, are necessary in order to clarify the exact mechanisms of action of vitamin C and vitamin E on oxidative stress response during endurance exercise.…”

Section: Resultsmentioning

confidence: 99%

Effects of L-Ascorbic Acid and Alpha-Tocopherol on Biochemical Parameters of Swimming-Induced Oxidative Stress in Serum of Guinea Pigs

Bursać-Mitrović¹,

Мilоvаnоvić²,

Mitić³

et al. 2016

Afr J Tradit Complement Altern Med

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Background: The purpose of this study is to determine the effect of L-ascorbic acid and alpha-tocopherol as well as combination of these vitamins with or without exposure to physical exercise on intensity of lipid peroxidation, activity of xanthine oxidase, activity of total antioxidative system, concentration of glutathione, and activity of catalase in the serum of guinea pigs. Materials and Methods: The experimental measurements of intensity of lipid peroxidation, activity of xanthine oxidase, activity of total antioxidative system, concentration of glutathione, and activity of catalase were done in the serum of guinea pigs. The animals were exposed to the test load to achieve exhaustion and the test was terminated when the animal for the third time to sink into the water. Results:The results of this study demonstrated that endurance exercise of guinea pigs induced oxidative stress response in terms of increased lipid peroxidation and activity of xanthine oxidase in the serum of experimental animals. Our study investigated the antioxidant activity of L-ascorbic acid and alpha-tocopherol also measuring three protective markers in the serum: total antioxidant activity, content of glutathione and activity of catalase. The results obtained show that the vitamins influence the concentrations of above mentioned biochemical parameters, which points out their protective effect of swimming-induced oxidative stress. Conclusion: Single or combined administration of L-ascorbic acid and alpha-tocopherol caused significant inhibition of these markers indicating the important antioxidant activity of the vitamins. Results lead to conclude that the combined treatments with vitamins with or without exposure to physical exercise showed the clear synergistic effect..

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β‐Carotene, α‐tocopherol and ascorbic acid: differential profile of antioxidant, inflammatory status and regulation of gene expression in human mononuclear cells of diabetic donors

Abstract: The vitamin complex has a dual effect, pro-oxidant and antioxidant, being also dose dependent with different profiles of cells of non-diabetic and type 1 diabetic patients.

Cited by 15 publications

References 40 publications

A prospective study of serum metabolites and glioma risk

A prospective study of serum metabolites and glioma risk

Oral administration of antioxidants improves skin wound healing in diabetic mice

Effects of L-Ascorbic Acid and Alpha-Tocopherol on Biochemical Parameters of Swimming-Induced Oxidative Stress in Serum of Guinea Pigs

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