Inheritance and QTL mapping of Fusarium wilt race 4 resistance in cotton

Abstract

Diseases such as Fusarium wilt [Fusarium oxysporum f.sp. vasinfectum (FOV) Atk. Sny & Hans] represent expanding threats to cotton production. Integrating disease resistance into high-yielding, high-fiber quality cotton (Gossypium spp.) cultivars is one of the most important objectives in cotton breeding programs worldwide. In this study, we conducted a comprehensive analysis of gene action in cotton governing FOV race 4 resistance by combining conventional inheritance and quantitative trait loci (QTL) mapping with molecular markers. A set of diverse cotton populations was generated from crosses encompassing multiple genetic backgrounds. FOV race 4 resistance was investigated using seven parents and their derived populations: three intraspecific (G. hirsutum × G. hirsutum L. and G. barbadense × G. barbadense L.) F1 and F2; five interspecific (G. hirsutum × G. barbadense) F1 and F2; and one RIL. Parents and populations were evaluated for disease severity index (DSI) of leaves, and vascular stem and root staining (VRS) in four greenhouse and two field experiments. Initially, a single resistance gene (Fov4) model was observed in F2 populations based on inheritance of phenotypes. This single Fov4 gene had a major dominant gene action and conferred resistance to FOV race 4 in Pima-S6. The Fov4 gene appears to be located near a genome region on chromosome 14 marked with a QTL Fov4-C14 1 , which made the biggest contribution to the FOV race 4 resistance of the generated F2 progeny. Additional genetic and QTL analyses also identified a set of 11 SSR markers that indicated the involvement of more than one gene and gene interactions across six linkage groups/chromosomes (3, 6, 8, 14, 17, and 25) in the inheritance of FOV race 4 resistance. QTLs detected with minor effects in these populations explained 5-19 % of the DSI or VRS variation. Identified SSR markers for the resistance QTLs with major and minor effects will facilitate for the first time marker-assisted selection for the introgression of FOV race 4 resistance into elite cultivars during the breeding process.

Development of EST-SSR markers related to disease resistance and their application in genetic diversity and evolution analysis in Gossypium

“…In cotton research, Ulloa et al (2013) comprehensively analyzed gene action in cotton governing Fusarium wilt (Fusarium oxysporum f sp vasinfectum, FOV) race 4 resistance by combining conventional inheritance and QTL mapping with molecular markers. They found that a single resistance gene (Fov4) had a major dominant gene action and conferred resistance to the FOV race 4 in Pima S6, which appears to be located on chromosome 14.…”

Mapping genomic loci for cotton plant architecture, yield components, and fiber properties in an interspecific (Gossypium hirsutum L. × G. barbadense L.) RIL population

“…Understanding the underlying genetic control of biological processes for favorable traits is relevant to a range of research objectives. Such understanding has potential applications for cotton crop improvement Cao et al 2014;Lacape et al 2013;Ning et al 2014;Rong et al 2007;Ulloa et al 2005Ulloa et al , 2013.…”

“…However, due to the allopolyploid nature of the cotton genome, certain chromosomal structures remain to be resolved. These include deletions, duplications, or rearrangements that may be specific to species, or to particular crosses within or between species (Brubaker et al 1999;Guo et al 2007;Lacape et al 2009;Rong et al 2004;Ulloa et al 2013;Wang et al 2013;Yu et al 2012a).…”

“…3-79). Using the single nonparametric and MQM QTL model mapping procedures, we detected 428 putative loci in the 159 genomic regions that confer 24 cotton traits in three diverse production environments [College Station F&B Road (FB), TX; Brazos 1 3 G. herbaceum L. (A 1 ) and G. arboreum L. (A 2 ), and two tetraploid species (2n = 4X = 52) from the New World, G. hirsutum L. (AD 1 ) and G. barbadense L. (AD 2 ), are cultivated to produce the world's leading natural fiber and the second most important oilseed crop (Campbell et al 2010;Kantartzi et al 2009;Lee 1984;Ulloa et al 2013;Wendel et al 1992;Percy et al 2014). The complexity of Gossypium genomes has afforded research opportunities on the evolution and diversity among diploid (Ma et al 2010) and allotetraploid species (Brubaker et al 1999;Jiang et al 1998;Paterson et al 2012).…”

Comparison of wild accessions of g. barbadense l. from Peru and Brazil via microsatellite markers

“…Twenty-nine microsatellite markers (SSR) were analyzed from the DNA samples. The SSR analysis was performed using the same conditions described by Ulloa et al (2013). From the molecular data, it was carried out analysis by descriptive statistics and analysis by methods based on distance index and on ancestry model.…”