CpG <scp>ODN</scp>‐induced matrix metalloproteinase‐13 expression is mediated via activation of the <scp>ERK</scp> and <scp>NF</scp>‐κB signalling pathways in odontoblast cells

Zhang, J.; Zhu, Qinglin; Huang, Ping; Yu, Qing; Wang, Z. H.; Cooper, Paul R.; Smith, Anthony J.; Wang, He

doi:10.1111/iej.12043

Cited by 17 publications

(14 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, we found a reduction of all analysed activation parameters induced by PPVO after inhibiting JNK signalling with SP600125 (Figure 4B). The JNK signalling cascade is known to be involved in the induction of pro-inflammatory cytokines by TLR [29], but the JNK inhibitor SP600125 only marginally influences CpG-ODN-induced matrixmetalloproteinase 13 production by odontoblasts or RANTES secretion by Langerhans cells [38], [39]. We demonstrate that SP600125 inhibits PPVO-mediated type I IFN induction by TLR9 in pDC.…”

Section: Discussionmentioning

confidence: 74%

Identification of Toll-Like Receptor 9 as Parapoxvirus Ovis-Sensing Receptor in Plasmacytoid Dendritic Cells

Buttlar

Siegemund

Büttner³

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

Parapoxvirus ovis (PPVO) is known for its immunostimulatory capacities and has been successfully used to generate vector vaccines effective especially in non-permissive host species. Murine conventional and plasmacytoid dendritic cells (cDC and pDC) are able to recognize PPVO. The PPVO-sensing receptor on pDC is hitherto unknown. In this study we aimed to define the pattern recognition receptor responsible for the activation of murine pDC by inactivated and replication-competent PPVO. We show that PPVO-induced expression of type I and type III interferons, pro-inflammatory cytokines, and co-stimulatory CD86 by bone marrow-derived pDC but not cDC is blocked by chloroquine, an inhibitor of endosomal maturation. The activation of pDC is independent of viral replication and depends mainly on TLR9. Moreover, the use of phosphatidylinositol 3-kinase inhibitor wortmannin or C-Jun-N-terminal kinase inhibitor SP600125 results in significant reduction of PPVO-induced pDC activation. Taken together, our data identify endosomal TLR9 as PPVO-sensing receptor in pDC.

show abstract

Section: Discussionmentioning

confidence: 74%

Identification of Toll-Like Receptor 9 as Parapoxvirus Ovis-Sensing Receptor in Plasmacytoid Dendritic Cells

Buttlar

Siegemund

Büttner³

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…TLR9 recognizes unmethylated CpG motifs, and then induces the recruitment of MyD88 to initiate downstream signaling (Takeshita et al, 2004). Furthermore, it has been shown that TLR9 associates with MyD88 to initiate CpG ODN mediated effects via signal-transducing pathways including NF-κB, p38 MAPK, JNK, AP-1, and ATF-2 (Akira et al, 2001; Osawa et al, 2006; Zhang et al, 2013). PBMCs express a wide range of TLRs and are crucial targets for various pathogens.…”

Section: Discussionmentioning

confidence: 99%

CpG Oligodeoxynucleotides Induce Differential Cytokine and Chemokine Gene Expression Profiles in Dapulian and Landrace Pigs

Yang

Wang

et al. 2016

Front. Microbiol.

View full text Add to dashboard Cite

Oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODN) mimic the immunostimulatory activity of microbial DNA by interacting with Toll-like receptor 9 (TLR9) to activate both the innate and adaptive immune responses in different species. However, few studies have been published to compare the effects of CpG ODN on different pig breeds. Therefore, in this study, whole blood gene expression profiles of DPL and Landrace pigs treated with CpG ODN were studied using RNA-seq technology. Five Hundred differentially expressed genes (DEGs) were identified between the two breeds. DPL pigs had significantly higher number of immune-relevant DEGs than the Landrace pigs after CpG ODN treatment. Pathway analysis showed that cytokine-cytokine receptor interaction and chemokine signaling pathway were the major enriched pathways of the immune-relevant DEGs. Further in vitro experiments showed that PBMCs of the DPL pigs had significantly higher levels of TLR9 mRNA than those of the Landrace pigs, both before and after CpG ODN stimulation. Cytokine and chemokine induction in the PBMCs of both breeds were also measured after CpG ODN stimulation. Our data showed that mRNA levels of cytokines (IFNα, IL8, IL12 p40) and chemokines (CXCL9, CXCL13) were significantly higher in the PBMCs of the DPL pigs than those of the Landrace pigs. Taken together, our data provide new information regarding the pig breed difference in response to CpG ODN stimulation and that higher levels of TLR9 mRNA in DPL pigs may be a major contributor for disease resistance.

show abstract

“…All the the mitogen-activated protein kinase (MAPK) pathways (ERK1/2, p38, and JNK) have been shown to be involved in MMP-13 expression in response to various stimuli and stress (Nakai et al, 2012; Onodera et al, 2002; Rossa et al, 2007; Zhang et al, 2012). However, the MAPK pathways which are critical in the LPS-induced MMP-13 gene regulation remain largely unknown.…”

Section: Resultsmentioning

confidence: 99%

“…MMP-13 expression can be regulated by MAPK in response to various stimuli and in different cells (Nakai et al, 2012; Onodera et al, 2002; Rossa et al, 2007; Zhang et al, 2012). However, how SOCS3 regulates MAPK in osteoblast is not known.…”

Section: Discussionmentioning

confidence: 99%

A critical role for suppressors of cytokine signaling 3 in regulating LPS-induced transcriptional activation of matrix metalloproteinase-13 in osteoblasts

Gao¹,

Kantarcı²,

Herrera³

et al. 2013

PeerJ

View full text Add to dashboard Cite

Suppressor of cytokine signaling 3 (SOCS3) is a key regulator of cytokine signaling in macrophages and T cells. Although SOCS3 seems to contribute to the balance between the pro-inflammatory actions of IL-6 family of cytokines and anti-inflammatory signaling of IL-10 by negatively regulating gp130/Jak/Stat3 signal transduction, how and the molecular mechanisms whereby SOCS3 controls the downstream impact of TLR4 are largely unknown and current data are controversial. Furthermore, very little is known regarding SOCS3 function in cells other than myeloid cells and T cells. Our previous study demonstrates that SOCS3 is expressed in osteoblasts and functions as a critical inhibitor of LPS-induced IL-6 expression. However, the function of SOCS3 in osteoblasts remains largely unknown. In the current study, we report for the first time that LPS stimulation of osteoblasts induces the transcriptional activation of matrix metalloproteinase (MMP)-13, a central regulator of bone resorption. Importantly, we demonstrate that SOCS3 overexpression leads to a significant decrease of LPS-induced MMP-13 expression in both primary murine calvariae osteoblasts and a mouse osteoblast-like cell line, MC3T3-E1. Our findings implicate SOCS3 as an important regulatory mediator in bone inflammatory diseases by targeting MMP-13.

show abstract

CpG ODN‐induced matrix metalloproteinase‐13 expression is mediated via activation of the ERK and NF‐κB signalling pathways in odontoblast cells

Abstract: The CpG ODN-induced MMP-13 expression in OLCs is mediated through TLR9, NF-κB and the ERK pathway indicating that potentially the recognition of CpG ODN by TLR9 on odontoblasts may regulate the remodelling of injured dental pulp and hard tissues by inducing MMP-13 expression.

Cited by 17 publications

References 36 publications

Identification of Toll-Like Receptor 9 as Parapoxvirus Ovis-Sensing Receptor in Plasmacytoid Dendritic Cells

Identification of Toll-Like Receptor 9 as Parapoxvirus Ovis-Sensing Receptor in Plasmacytoid Dendritic Cells

CpG Oligodeoxynucleotides Induce Differential Cytokine and Chemokine Gene Expression Profiles in Dapulian and Landrace Pigs

A critical role for suppressors of cytokine signaling 3 in regulating LPS-induced transcriptional activation of matrix metalloproteinase-13 in osteoblasts

Contact Info

Product

Resources

About