Calcium channel blockers protect against aluminium-induced DNA damage and block adaptive response to genotoxic stress in plant cells

Achary, V. Mohan Murali; Parinandi, Narasimham L.; Panda, Brahma B.

doi:10.1016/j.mrgentox.2012.12.008

Cited by 39 publications

(25 citation statements)

References 67 publications

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“…When the Al cation enters the plants, it is extremely reactive and also highly immobile. Therefore, as its primary effect, growth of the root tip at the entry site is rapidly affected by inhibition of mitosis and DNA damage in the nuclei (Clarkson, 1965;Achary et al, 2013). Al has further many other target sites of inhibition, these include disruption of the negatively charged cell wall and plasmalemma, displacement of Ca 2þ or Mg 2þ in the cell wall, in the transmembrane transporters or in enzymes within the cytoplasm, disruption of the cytoskeletal elements (microtubules and actin filaments), interference with Ca 2þ homoeostasis and signal transduction pathways, and induction of oxidative stress (generation of reactive oxygen species, ROS), etc (Kochian et al, 2004(Kochian et al, , 2005.…”

Section: Aluminium Toxicitymentioning

confidence: 99%

The lime–silicate question

Bothe

2015

Soil Biology and Biochemistry

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Section: Aluminium Toxicitymentioning

confidence: 99%

The lime–silicate question

Bothe

2015

Soil Biology and Biochemistry

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“…The involvement of checkpoint regulators such as TANMEI/ALUMINUM TOLERANT2 (ALT2) and ataxia telangiectasia-mutated (ATM) and ATM-Rad3-related (ATR) kinases in the Al-dependent root growth inhibition and cell cycle arrest in Arabidopsis thaliana has been suggested (Rounds and Larsen, 2008; Nezames et al, 2012). Furthermore, we have recently demonstrated the role of ROS in Ca 2+ channel signal transduction underlying Al 3+ -induced DNA damage and adaptive response (Achary et al, 2013). Also, in this study, we have shown that the Al 3+ induces adaptive response to genotoxic stress in root cells of A. cepa failing to uphold the cell cycle checkpoint arrest mechanism in the underlying DNA damage and response.…”

Section: Introductionmentioning

confidence: 99%

Mitogen-activated protein kinase signal transduction and DNA repair network are involved in aluminum-induced DNA damage and adaptive response in root cells of Allium cepa L.

Panda

Achary

2014

Front. Plant Sci.

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In the current study, we studied the role of signal transduction in aluminum (Al3+)-induced DNA damage and adaptive response in root cells of Allium cepa L. The root cells in planta were treated with Al3+ (800 μM) for 3 h without or with 2 h pre-treatment of inhibitors of mitogen-activated protein kinase (MAPK), and protein phosphatase. Also, root cells in planta were conditioned with Al3+ (10 μM) for 2 h and then subjected to genotoxic challenge of ethyl methane sulfonate (EMS; 5 mM) for 3 h without or with the pre-treatment of the aforementioned inhibitors as well as the inhibitors of translation, transcription, DNA replication and repair. At the end of treatments, roots cells were assayed for cell death and/or DNA damage. The results revealed that Al3+ (800 μM)-induced significant DNA damage and cell death. On the other hand, conditioning with low dose of Al3+ induced adaptive response conferring protection of root cells from genotoxic stress caused by EMS-challenge. Pre-treatment of roots cells with the chosen inhibitors prior to Al3+-conditioning prevented or reduced the adaptive response to EMS genotoxicity. The results of this study suggested the involvement of MAPK and DNA repair network underlying Al-induced DNA damage and adaptive response to genotoxic stress in root cells of A. cepa.

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“…Allium cepa L. has been used to evaluate DNA damage, such as chromosome aberrations, micronuclei and disturbances in the mitotic cycle (Bakare et al 2012;Frescura et al 2012;Olorunfemi et al 2012;Achary et al 2013). Due to its sensitivity, the A. cepa test was the first of nine plants assay systems evaluated by the Gene-Tox Program of the US Environmental Protection Agency (Grant 1994).…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic effects of chitosan-capped silver nanoparticles in theAllium cepatest

Pesnya

2013

Caryologia

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Silver nanoparticles are one of the most commonly used nanomaterials. However, there remains insufficient information on their genotoxic effects. The goal of this study was to investigate cytotoxic and genotoxic effects of chitosan-coated AgNPs using the well-established Allium test. Root tip cells were treated with solutions at different concentrations (1, 2.5, 5 and 50 mg l . The absence of induction of chromosomal and mitotic abnormalities by chitosan-capped AgNPs at low concentrations is possibly due to the capping, which may partly protect the cells from direct interaction with the AgNPs. Mitotic and chromosomal abnormalities and micronuclei were detected at a concentration of 50 mg l −1 . Significant increase in mitotic index was found at 5 and 50 mg l −1 concentrations of AgNP. The data demonstrated that chitosan-coated AgNP exhibit both clastogenic and aneugenic activity.

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Calcium channel blockers protect against aluminium-induced DNA damage and block adaptive response to genotoxic stress in plant cells

Cited by 39 publications

References 67 publications

The lime–silicate question

The lime–silicate question

Mitogen-activated protein kinase signal transduction and DNA repair network are involved in aluminum-induced DNA damage and adaptive response in root cells of Allium cepa L.

Cytogenetic effects of chitosan-capped silver nanoparticles in theAllium cepatest

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