Prolonged synthesis of hyaluronan by Chlorella cells infected with chloroviruses

Rakkhumkaew, Numfon; Kawasaki, Takeru; Fujie, Makoto; Yamada, Takashi

doi:10.1016/j.jbiosc.2012.11.012

Cited by 5 publications

(3 citation statements)

References 26 publications

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“…HA synthesis by microalgal Chlorella cells occurs upon infection with chloroviruses [ 48 , 52 ]. PBCV-1-infected chlorella was monitored for HA synthesis, and studies indicated that about 80 % produce HA during infection [ 53 ].…”

Section: Main Textmentioning

confidence: 99%

“…In general, the production (maximum of 1 g/L, JP patent 2004-283096) does not reach the values typically achieved using Streptococcal fermentation (Table 2 ). However, recent methods were proposed to improve the yield of HA production in Chlorella: (1) using isolated chlorovirus cells that have low growth rate (CV01 and CVTS1) and (2) affecting the ability of cells to reproduce by using molecules capable of inhibit DNA synthesis inside the cell, and aphidicolin [ 52 ]. The use of the two approaches together allowed for a concentration of 14 µg/ml, about a 2.2-fold increase over PBCV-1 infected chlorella, which showed a concentration of 6.3 µg/mL HA.…”

Section: Main Textmentioning

confidence: 99%

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Genetic basis for hyper production of hyaluronic acid in natural and engineered microorganisms

Oliveira

Carvalho²,

Gomes

et al. 2016

Microb Cell Fact

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Hyaluronic acid, or HA, is a rigid and linear biopolymer belonging to the class of the glycosaminoglycans, and composed of repeating units of the monosaccharides glucuronic acid and N-acetylglucosamine. HA has multiple important functions in the human body, due to its properties such as bio-compatibility, lubricity and hydrophilicity, it is widely applied in the biomedical, food, health and cosmetic fields. The growing interest in this molecule has motivated the discovery of new ways of obtaining it. Traditionally, HA has been extracted from rooster comb-like animal tissues. However, due to legislation laws HA is now being produced by bacterial fermentation using Streptococcus zooepidemicus, a natural producer of HA, despite it being a pathogenic microorganism. With the expansion of new genetic engineering technologies, the use of organisms that are non-natural producers of HA has also made it possible to obtain such a polymer. Most of the published reviews have focused on HA formulation and its effects on different body tissues, whereas very few of them describe the microbial basis of HA production. Therefore, for the first time this review has compiled the molecular and genetic bases for natural HA production in microorganisms together with the main strategies employed for heterologous production of HA.

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Section: Main Textmentioning

confidence: 99%

Section: Main Textmentioning

confidence: 99%

Genetic basis for hyper production of hyaluronic acid in natural and engineered microorganisms

Oliveira

Carvalho²,

Gomes

et al. 2016

Microb Cell Fact

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“…One would expect the polysaccharide fibers to bunch up underneath the cell wall. In fact, this happened when the viral has gene was expressed in cultured tobacco cells [31]. Could a pilot protein(s) that is attached to the leading end of the polymer guide the hyaluronan chain through the wall?…”

Section: Chlorovirus Encoded Polysaccharide Synthesizing Enzymesmentioning

confidence: 99%

Chloroviruses Have a Sweet Tooth

Etten

Agarkova

Dunigan

et al. 2017

Viruses

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Chloroviruses are large double-stranded DNA (dsDNA) viruses that infect certain isolates of chlorella-like green algae. They contain up to approximately 400 protein-encoding genes and 16 transfer RNA (tRNA) genes. This review summarizes the unexpected finding that many of the chlorovirus genes encode proteins involved in manipulating carbohydrates. These include enzymes involved in making extracellular polysaccharides, such as hyaluronan and chitin, enzymes that make nucleotide sugars, such as GDP-l-fucose and GDP-d-rhamnose and enzymes involved in the synthesis of glycans attached to the virus major capsid proteins. This latter process differs from that of all other glycoprotein containing viruses that traditionally use the host endoplasmic reticulum and Golgi machinery to synthesize and transfer the glycans.

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Transgenic eukaryotic microalgae as green factories: providing new ideas for the production of biologically active substances

et al. 2021

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Prolonged synthesis of hyaluronan by Chlorella cells infected with chloroviruses

Cited by 5 publications

References 26 publications

Genetic basis for hyper production of hyaluronic acid in natural and engineered microorganisms

Genetic basis for hyper production of hyaluronic acid in natural and engineered microorganisms

Chloroviruses Have a Sweet Tooth

Transgenic eukaryotic microalgae as green factories: providing new ideas for the production of biologically active substances

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