Transgenic eukaryotic microalgae as green factories: providing new ideas for the production of biologically active substances

Shi, Qianwen; Chen, Cheng; Zhang, Wei; Wu, Peixuan; Sun, Meng; Wu, Hui; Wu, Haizhen; Fu, Pengcheng; Fan, Jianhua

doi:10.1007/s10811-020-02350-7

Cited by 20 publications

(14 citation statements)

References 221 publications

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“…The variation in band intensity showed that protein differed qualitatively and quantitatively among species. This result agreed with those previous studies that reported not only differences in the expression and assembly of the available protein in algae (Mayfield et al 2003 ; Wang et al 2014 ; Shi et al 2021 ) but also de novo synthesized proteins in unicellular green-alga-like Scenedesmus phlyctidium upon external applications (Nedeva et al 2008 ). So we suggest that qualitative and quantitative variation in band intensity among species can indicate taxonomy and the potentiality of toxicity level upon expression level in toxic species, respectively.…”

Section: Discussionsupporting

confidence: 92%

Section: Polymorphism and Phylogenetic Relations Based On Protein Pro...supporting

confidence: 92%

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Molecular taxonomical identification and phylogenetic relationships of some marine dominant algal species during red tide and harmful algal blooms along Egyptian coasts in the Alexandria region

El-Hadary

Elsaied

Khalil

et al. 2022

Environ Sci Pollut Res

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Harmful algal blooms (HABs) threaten the aquatic ecosystems due to either poisonous effects on living organisms or oxygen-consuming. So HABs’ accurate identification, including red tide, is crucial. This study aimed to molecular identification of dominant species during tide period in nine stations along Alexandria region at Egyptian costs during one year. Samples were collected weekly before water discoloration but daily during red tide intensive growth from both 50 cm below the surface and 3 m depth over the bottom from the water surface. The red tide detection was highly from early August to half of September, since its highest peak with a maximum frequency inside the Eastern Harbor. The examined cultures samples isolated during red tide had four dominant species. Peroxidase profile showed an expression pattern of three loci (Px1, Px2, and Px3) in most species. The Px2 was the only heterozygous locus among the three loci in all species. Protein profiling showed that 17 bands out of 65 were specific to the species. The phylogenetic relationships derived from profiles of protein and 18S rRNA gene operon sequences for the four isolated species were mostly similar. We identified the four dominant HABs species as Aplanochytrium sp., Chlamydomonas sp., Cryptophyceae sp., and Psammodictyon sp. based on their 18S rRNA sequences and deposited them at DDBJ/EMBL/GenBank database. Aplanochytrium sp. is recorded as a red tide causative species for the first time in the screened region despite belonging to the defunct fungi.

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Section: Discussionsupporting

confidence: 92%

Section: Polymorphism and Phylogenetic Relations Based On Protein Pro...supporting

confidence: 92%

Molecular taxonomical identification and phylogenetic relationships of some marine dominant algal species during red tide and harmful algal blooms along Egyptian coasts in the Alexandria region

El-Hadary

Elsaied

Khalil

et al. 2022

Environ Sci Pollut Res

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“…Currently, antibiotic abuse is a prominent problem in aquaculture [24]. Among the possible solutions to resolve this issue, oral administration of microalgae that contain recombinant antibacterial peptides or vaccines is a promising solution [25].…”

Section: Discussionmentioning

confidence: 99%

Recombinant Expression of Thrombolytic Agent Reteplase in Marine Microalga Tetraselmis subcordiformis (Chlorodendrales, Chlorophyta)

Zheng

Wang

et al. 2021

Marine Drugs

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Tetraselmis subcordiformis, a unicellular marine green alga, is used widely in aquaculture as an initial feeding for fish, bivalve mollusks, penaeid shrimp larvae, and rotifers because of its rich content of amino acids and fatty acids. A stable nuclear transformation system using the herbicide phosphinothricin (PPT) as a selective reagent was established previously. In this research, the recombinant expression in T. subcordiformis was investigated by particle bombardment with the rt-PA gene that encodes the recombinant human tissue-type plasminogen activator (Reteplase), which is a thrombolytic agent for acute myocardial infarction treatment. Transgenic algal strains were selected by their resistance to PPT, and expression of rt-PA was validated by PCR, Southern blotting, and Western blotting, and bioactivity of rt-PA was confirmed by the fibrin agarose plate assay for bioactivity. The results showed that rt-PA was integrated into the genome of T. subcordiformis, and the expression product was bioactive, indicating proper post-transcriptional modification of rt-PA in T. subcordiformis. This report contributes to efforts that take advantage of marine microalgae as cell factories to prepare recombinant drugs and in establishing a characteristic pathway of oral administration in aquaculture.

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“…Therefore, many AMPs seem to be less susceptible to the development of resistances and are denominated “next generation antibiotics” (Spohn et al 2019 ). Several potent AMPs for application in aquaculture have already been produced in Chlamydomonas (Shi et al 2021 ). Bovine lactoferricin represents the first AMP synthesized by engineered Nannochloropsis .…”

Section: Biopharmaceuticals Produced By Stramenopiles For Aquaculture and Human Medicationmentioning

confidence: 99%

Stramenopile microalgae as “green biofactories” for recombinant protein production

Grahl

Reumann

2021

World J Microbiol Biotechnol

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Photoautotrophic microalgae have become intriguing hosts for recombinant protein production because they offer important advantages of both prokaryotic and eukaryotic expression systems. Advanced molecular tools have recently been established for the biotechnologically relevant group of stramenopile microalgae, particularly for several Nannochloropsis species and diatoms. Strategies for the selection of powerful genetic elements and for optimization of protein production have been reported. Much needed high-throughput techniques required for straight-forward identification and selection of the best expression constructs and transformants have become available and are discussed. The first recombinant proteins have already been produced successfully in stramenopile microalgae and include not only several subunit vaccines but also one antimicrobial peptide, a fish growth hormone, and an antibody. These research results offer interesting future applications in aquaculture and as biopharmaceuticals. In this review we highlight recent progress in genetic technology development for recombinant protein production in the most relevant Nannochloropsis species and diatoms. Diverse realistic biotechnological applications of these proteins are emphasized that have the potential to establish stramenopile algae as sustainable green factories for an economically competitive production of high-value biomolecules.

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Transgenic eukaryotic microalgae as green factories: providing new ideas for the production of biologically active substances

Cited by 20 publications

References 221 publications

Molecular taxonomical identification and phylogenetic relationships of some marine dominant algal species during red tide and harmful algal blooms along Egyptian coasts in the Alexandria region

Molecular taxonomical identification and phylogenetic relationships of some marine dominant algal species during red tide and harmful algal blooms along Egyptian coasts in the Alexandria region

Recombinant Expression of Thrombolytic Agent Reteplase in Marine Microalga Tetraselmis subcordiformis (Chlorodendrales, Chlorophyta)

Stramenopile microalgae as “green biofactories” for recombinant protein production

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