Establishment of a Model of Streptococcus iniae Meningoencephalitis in Nile Tilapia (Oreochromis niloticus)

Baums, Christoph Georg; Hermeyer, Kathrin; Leimbach, Stefanie; Adamek, Mikołaj; Czerny, Claus-Peter; Hörstgen‐Schwark, Gabriele; Valentin‐Weigand, Peter; Baumgartner, Wes; Steinhagen, Dieter

doi:10.1016/j.jcpa.2012.10.003

Cited by 40 publications

(23 citation statements)

References 19 publications

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“…Finally, eye tissue presented severe granulomatous endophthalmitis (Figure e), choroiditis and corneal oedema, as well as severe haemorrhagic and necrotic granulomatous myositis (Figure f). These findings are consistent with the development process of bacterial septicaemia, with similar histological descriptions existing for Streptococcus bacteria in tilapia (Baums et al., ; Chen et al., ; Zamri‐Saad et al., ).…”

Section: Resultssupporting

confidence: 90%

“…Furthermore, the hearts of five San Luis Potosí fish were surrounded by white‐greyish, purulent‐mass membranes (Figure f). The pathological picture for San Luis Potosí and Querétaro fish, for both internal and external wounds, clearly corresponded to the description of a typical streptococcosis process in tilapia (Baums et al., ; Chen et al., ; Ramesh et al., ; Zamri‐Saad et al., ), which was apparently more chronic at the San Luis Potosí farm.…”

Section: Resultssupporting

confidence: 77%

“…Eight of ten San Luis Potosí specimens and ten of ten Querétaro specimens evidenced macroscopic lesions typical of septicaemia. Notable among these were corneal opacity and exophthalmia (Figure a) and a poor body condition, symptoms in line with the pathological processes caused by bacteria of the Streptococcus genus (Baums et al., ; Chen, Chao, & Bowser, ; Zamri‐Saad et al., ). When cases tended towards chronicity, as in the San Luis Potosí sample group, the affected animals presented a poor body condition and, even, endoftalmitis.…”

Section: Resultsmentioning

confidence: 93%

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First identification and characterization of Streptococcus iniae obtained from tilapia (Oreochromis aureus) farmed in Mexico

Ortega

Garcia

Irgang

et al. 2018

Journal of Fish Diseases

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This is the first study to isolate, identify and characterize Streptococcus iniae as the causative disease agent in two tilapia (Oreochromis aureus) populations. The populations were geographically isolated, of distinct origins, and did not share water sources. Affected fish showed various external (e.g., exophthalmia and cachexia, among others) and internal (e.g., granulomatous septicaemia and interstitial nephritis, among others) signs. All internal organ samples produced pure cultures, two of which (one from each farm, termed S-1 and S-2) were subjected to biochemical, PCR and 16S rRNA sequencing (99.5% similarity) analyses, confirming S. iniae identification. The two isolates presented genetic homogeneity regardless of technique (i.e., RAPD, REP-PCR and ERIC-PCR analyses). Pathogenic potentials were assessed through intraperitoneal injection challenges in rainbow trout (Oncorhynchus mykiss) and zebrafish (Danio rerio). Rainbow trout mortalities were respectively 40% and 70% at 10 and 10 CFU per fish with the S-1 isolate, while 100% mortality rates were recorded in zebrafish at 10 and 10 CFU per fish with the S-2 isolate. The obtained data clearly indicate a relationship between intensified aquaculture activities in Mexico and new disease appearances. Future studies should establish clinical significances for the tilapia industry.

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Section: Resultssupporting

confidence: 90%

Section: Resultssupporting

confidence: 77%

Section: Resultsmentioning

confidence: 93%

See 1 more Smart Citation

First identification and characterization of Streptococcus iniae obtained from tilapia (Oreochromis aureus) farmed in Mexico

Ortega

Garcia

Irgang

et al. 2018

Journal of Fish Diseases

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“…), showing amplification 822 bp for scpI , 994 bp for simA , 713 bp for pgm , 534 bp for cpsD , 381 bp for pdi and 190 bp for sagA , which were identical to that produced in the strain S. iniae K288 (Baums et al . ).…”

Section: Resultsmentioning

confidence: 97%

“…In the study, two strains were investigated for six main virulence genes ( simA , scpI , pdi , pgm , cpsD and sagA , Table ) of S. iniae by a multiplex PCR (Baums, Hermeyer, Leimbach, Adamek, Czerny, Hörstgen Schwark, Valentin Weigand, Baumgärtner & Steinhagen ). The amplification was performed using a reaction mixture which contained PCR buffer 10×, 1.5 mM MgCl 2 , 0.2 mM each of the four deoxynucleotide triphosphates, 2.0 U TaqDNA polymerase (Takara, Dalian, China), 116 nM of each primer for simA , 58 nM of each primer for scpI, pgm and cpsD , 93 nM of each primer for pdi , 116 nM of each primer for sagA and 5 μg of template DNA in a final volume of 50 μL in a thermal cycler (C1000; Bio‐Rad, Hercules, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Outbreaks of Streptococcosis associated withStreptococcus iniaein Siberian sturgeon (Acipenser baerii) in China

Deng

Geng

et al. 2015

Aquac Res

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Streptococcus iniae has emerged as an important fish pathogen over the past few decades causing high losses in aquaculture farms all over the world. At least 27 species of fish have been documented to be infected by S. iniae, including cultured and wild populations. In August and October 2013, a serious infectious disease characterized by body ulcer, internal organs haemorrhages and nodules showing on epicardium occurred on the Acipenser baerii farms in Ya'an country, China. Histological examination revealed a multisystemic, necrotising inflammatory response that was particularly marked in liver, kidney, heart and brain. Mass mortality (>40%) was observed in infected fish and two Gram-positive cocci (Ab130920 and Ab131025) were obtained from kidneys and livers of diseased fish. Experimental infections with these two isolates resulted in marked symptoms in the sturgeons similar to those observed in natural outbreaks, and the LD 50 values of the two isolates were 5.1 9 10 5 and 6.4 9 10 5 cfu per fish respectively. The two microorganisms were identified as S. iniae through physiological and biochemical tests, 16S rRNA and lctO gene sequence analysis. Both two isolates showed a similar antibiotic susceptibility, which were sensitive to ampicillin, amoxicillin, cefazolin, amikacin, deoxycycline, florfenicol, azithromycin, ciprofloxacin, vancomycin and resistant to streptomycin, gentamicin, kanamycin, norfloxacin and sinomin (SMZ/TMP). Multiplex PCR assay for virulence genes showed both isolates possessed six main virulence genes: simA, scpI, pdi, pgm, cpsD and sagA genes. These results indicated that S. iniae could act as a pathogen of farmed A. baerii. This is the first report of S. iniae infection associated with mass mortality in A. baerii.

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Epidemiology and Pathogenicity of Zoonotic Streptococci

Fulde

Valentin‐Weigand

2012

Current Topics in Microbiology and Immunology

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Zoonotic infections caused by Streptococcus spp. have been neglected in spite of the fact that frequency and severity of outbreaks increased dramatically in recent years. This may be due to non-identification since respective species are often not considered in human medical diagnostic procedures. On the other hand, an expanding human population concomitant with an increasing demand for food and the increased number of companion animals favour conditions for host species adaptation of animal streptococci. This review aims to give an overview on streptococcal zoonoses with focus on epidemiology and pathogenicity of four major zoonotic species, Streptococcus canis, Streptococcus equi sub. zooepidemicus, Streptococcus iniae and Streptococcus suis.

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Establishment of a Model of Streptococcus iniae Meningoencephalitis in Nile Tilapia (Oreochromis niloticus)

Cited by 40 publications

References 19 publications

First identification and characterization of Streptococcus iniae obtained from tilapia (Oreochromis aureus) farmed in Mexico

First identification and characterization of Streptococcus iniae obtained from tilapia (Oreochromis aureus) farmed in Mexico

Outbreaks of Streptococcosis associated withStreptococcus iniaein Siberian sturgeon (Acipenser baerii) in China

Epidemiology and Pathogenicity of Zoonotic Streptococci

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