A simple one-step assay platform based on fluorescence quenching of macroporous silicon

Yoo, Lina I.; Ahn, Kwangseog; Ahn, Ji Young; Laurell, Thomas; Lee, Yong Man; Yoo, Pil J.; Lee, Jeewon

doi:10.1016/j.bios.2012.09.012

Cited by 17 publications

(6 citation statements)

References 52 publications

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“…Likewise utilizing multifunctional porous silicon substrates, Yoo et al demonstrated a biosensor for the detection of adenosine [33]. Herein, the authors take advantage of the target induced dissociation of a TAMRA-labeled complementary strand from the aptamer-functionalized porous silicon surface.…”

Section: Biosensingmentioning

confidence: 99%

Aptamer-modified nanomaterials: principles and applications

et al. 2016

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Aptamers are promising alternative binders that can substitute antibodies in various applications. Due to the advantages of aptamers, namely their high affinity, specificity and stability, along with the benefits originating from the chemical synthesis of aptamers, they have attracted attention in various applications including their use on nanostructured material. This necessitates the immobilization of aptamers on a solid support. Since aptamer immobilization may interfere with its binding properties, the immobilization of aptamers has to be investigated and optimized. Within this review, we give general insights into the principles and factors controlling the binding affinity of immobilized aptamers. Specific features of aptamer immobilization on nanostructured surfaces and nanoparticles are highlighted and a brief overview of applications of aptamer-modified nanostructured materials is given.

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Section: Biosensingmentioning

confidence: 99%

Aptamer-modified nanomaterials: principles and applications

et al. 2016

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“…This combination of fluorescence quenching renders the biosensor highly effective to sensitive target detection and indeed, the proposed platform showed reliable detection in sub-micromolar concentrations. While basal adenosine blood-levels range from 20 to 200 nM [109], hypoxic tissue [92] or inflammatory response easily boost the concentrations to the 10-100 fold [110,111]. Therefore, the presented assay has the potential to differentiate between normal blood-level and increased adenosine concentrations.…”

Section: Optical Biosensorsmentioning

confidence: 94%

“…While there are certainly many more publications to be expected within the next years, in this review, we focus on few recent studies, which have demonstrated promising results. The first example of a fluorescence-based optical biosensor employing PSi in combination with novel capture probes, is a one-step assay presented by Yoo et al [92]. In this assay, a macroporous PSi is modified with streptavidin through physical adsorption followed by the introduction of biotin-conjugated DNA aptamer, directed against the target adenosine.…”

Section: Optical Biosensorsmentioning

confidence: 99%

“…The complex photoluminescence mechanisms, associated with electron transfer and interfacial charging [1,95,96], pose a major challenge in the development of reliable biosensors [97]. Yet, a recent study demonstrated the quenching of a reporter-label inside the porous structure in a very interesting manner [92], this work will be discussed in more detail in the next section. Fluorescent labels have also been used in assays to combine both, a fluorescent signal and reflectivity spectra [98] or electrical impedance [87], respectively.…”

Section: Optical Biosensorsmentioning

confidence: 99%

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Porous Silicon Biosensors Employing Emerging Capture Probes

Urmann

Tenenbaum

Walter

et al. 2015

Electrochemically Engineered Nanoporous Materials

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The application of porous silicon (PSi) for biosensing was first described by Thust et al. in 1996, demonstrating a potentiometric biosensor for the detection of penicillin. However, only in the past decade PSi has established as a promising nanomaterial for label-free biosensing applications. This chapter focuses on the integration of new emerging capture probes with PSi-based biosensing schemes. An overview of natural and synthetic receptors and their advantageous characteristics for the potential application in PSi biosensors technology is presented. We also review and discuss several examples, which successfully combine these new bioreceptors with PSi optical and electrochemical transducers, for label-free biosensing.

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“…Recently, a variety of new strategies have been established based on this turn off-on or quenching to recovery pattern [30][31][32][33][34]. By coating with an ultrathin dielectric layer of tetrahedral amorphous carbon (ta-C) on the Au nanoparticle substrates, the metal enhanced fluorescence could be obtained [35].…”

Section: Introductionmentioning

confidence: 99%