20β-Hydroxy-steroid-dehydrogenase, ein neues kristallines enzym

Hübener, H. J.; Sahrholz, F.G.; Schmidt-Thomé, Josef; Nesemann, G; Junk, Rudolf

doi:10.1016/0006-3002(59)90366-x

Cited by 45 publications

(4 citation statements)

References 4 publications

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“…There is a profound similarity between the reactions catalyzed by these enzymes. Cyclopentanol dehydrogenase and 3-α (or 20-β)-hydroxysteroid dehydrogenase, while active on seemingly different substrates, both convert an alicyclic-bound alcohol group to a ketone, producing reduced NADH as a product (54–56). The conversion of sterols to ketones is illustrated by the reaction catalyzed by 3-α (or 20-β)-hydroxysteroid dehydrogenase.…”

Section: Resultsmentioning

confidence: 99%

SAR202 Genomes from the Dark Ocean Predict Pathways for the Oxidation of Recalcitrant Dissolved Organic Matter

Landry

Swan

Herndl

et al. 2017

mBio

153

226

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Deep-ocean regions beyond the reach of sunlight contain an estimated 615 Pg of dissolved organic matter (DOM), much of which persists for thousands of years. It is thought that bacteria oxidize DOM until it is too dilute or refractory to support microbial activity. We analyzed five single-amplified genomes (SAGs) from the abundant SAR202 clade of dark-ocean bacterioplankton and found they encode multiple families of paralogous enzymes involved in carbon catabolism, including several families of oxidative enzymes that we hypothesize participate in the degradation of cyclic alkanes. The five partial genomes encoded 152 flavin mononucleotide/F420-dependent monooxygenases (FMNOs), many of which are predicted to be type II Baeyer-Villiger monooxygenases (BVMOs) that catalyze oxygen insertion into semilabile alicyclic alkanes. The large number of oxidative enzymes, as well as other families of enzymes that appear to play complementary roles in catabolic pathways, suggests that SAR202 might catalyze final steps in the biological oxidation of relatively recalcitrant organic compounds to refractory compounds that persist.

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Section: Resultsmentioning

confidence: 99%

SAR202 Genomes from the Dark Ocean Predict Pathways for the Oxidation of Recalcitrant Dissolved Organic Matter

Landry

Swan

Herndl

et al. 2017

mBio

153

226

View full text Add to dashboard Cite

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“…NAD diaphorase and NADP diaphorase are both histochemically demonstrable in the tissues exhibiting 20/?-hydroxysteroid dehydrogenase activity (unpublished observations) and it therefore seems likely that the specificity for NAD resides with the steroid dehydrogenase and is not secondary to a lack of NADP diaphorase. 20/?-Hydroxysteroid dehydrogenase was first isolated and crystallized from bacterial sources (Hübener, Sahrholtz, Schmidt-Thomé, Nesemann & Junk, 1959;Hiibener & Sahrholtz, 1960) and is known to be a NAD-linked enzyme. Its pH optimum in this histochemical reaction seems to lie between 7-0 and 7-4.…”

Section: Resultsmentioning

confidence: 99%

HISTOCHEMICAL DEMONSTRATION OF 20β-Hydroxysteroid DEHYDROGENASE

Baillie¹,

Calman²,

Ferguson³

et al. 1965

Journal of Endocrinology

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“…(Many 1-dehydrogenating microorganisms also bring about this reduction.) The enzyme itself, diphosphopyridine nucleotide-dependent 20-,B-hydroxysteroid dehydrogenase, is inducible and was obtained in crystalline form from Streptomyces hydrogenans (Hiibener and Sahrholz, 1960;Nesemann et al, 1960).…”

Section: Resultsmentioning

confidence: 99%

INTERMEDIATES IN THE METABOLISM OF STEROIDS BY PENICILLIUM LILACINUM

1962

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Nonproliferating cells of Penicillium lilacinum (Thom) metabbolized progesterone successively to 20-1hydroxy-4-pregnen-3-one, testosterone, androstenedione, and testololactone. Testosterone and androstenedione were readily interconvertible. 1 1-a-Hydroxyprogesterone was transformed in an analogous way and its intermediate, 11-a,20-fldihydroxy-4-pregnen-3-one, was isolated and identified by a combination of microbiological and chemical methods.

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20β-Hydroxy-steroid-dehydrogenase, ein neues kristallines enzym

Cited by 45 publications

References 4 publications

SAR202 Genomes from the Dark Ocean Predict Pathways for the Oxidation of Recalcitrant Dissolved Organic Matter

SAR202 Genomes from the Dark Ocean Predict Pathways for the Oxidation of Recalcitrant Dissolved Organic Matter

HISTOCHEMICAL DEMONSTRATION OF 20β-Hydroxysteroid DEHYDROGENASE

INTERMEDIATES IN THE METABOLISM OF STEROIDS BY PENICILLIUM LILACINUM

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