Arginomycinis a new nucleoside antibiotic produced by Streptomyces arginensis. Arginomycin, C18H28N8O5, which inhibits the growth of Gram-positive bacteria and fungi in vitro, is structurally related to blasticidin S and found to be relatively non-toxic.Cultures of Streptomyces arginensis UC8632, grown in a complex medium were found to contain an antibacterial agent which was characterized by its paper chromatographic and TLCbehavior and its antibacterial spectrum. This antibiotic which exhibited activity against both Gram-positive bacteria and fungi was isolated and designated arginomycin (U-72,026). The present communication deals with the production, isolation, characterization and the chemical structure of arginomycin.
Experimental
Assay and Testing ProceduresAntibiotic production and purification was measured by a microbiological disc-plate assay procedure with Micrococcus luteus or Penicillium oxalicum as the assay organism.
Paper Chromatographic ProceduresThe production of arginomycin was followed by paper chromatography using a variety of mobile phases. The Rf values of arginomycin in the following solvents were: 0.00 [1-butanol -water (84 : 16)
Thin-layer Chromatographic (TLC) ProceduresTLC's of arginomycin were run on cellulose (Brinkman Cell 300) plates using pH 7.0, 0.1 m phosphate buffer as the mobile phase. Bioautography was on M. luteus or P. oxalicum-seeded trays. Hydrolytic reactions on arginomycin were followed by TLCusing silica gel and chloroform -methanolcone ammoniumhydroxide (1 :4 : 1) as the mobile phase. Spots were developed by spraying with ninhydrin or using iodine vapors. UV absorbing materials were detected by a shortwave UV lamp.High Performance Liquid Chromatography (HPLC) All HPLCwas carried out using a C-18 reverse phase silica column on a Varian 5560 instrument equipped with an LKB-RSBphoto diode array UVdetector and operating in the dual pump mode.The mobile phase consisted of a 10 mMsolution of hexanesulfonic acid in acetonitrile -water (80 : 20) containing 0.2% glacial acetic acid. Flow rate 1.5 ml/minute. Sample volume 10 u\.
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