2,5-Hexanedione inhibits rat brain acetylcholinesterase activity in vitro

Pereira, Maria Estér; Adams, Andréa Inês Horn; Silva, Nélson S

doi:10.1016/j.toxlet.2003.10.009

Cited by 32 publications

(14 citation statements)

References 27 publications

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“…In fact, the effect per se of some organic solvents on AChE activity has been partially observed in the literature (11,(22)(23)(24). However, is lacking the complete curve inhibition in the principal brain structures.…”

Section: Discussionmentioning

confidence: 98%

Effects per se of Organic Solvents in the Cerebral Acetylcholinesterase of Rats

et al. 2005

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Acetylcholinesterase (AChE) was studied in different rat brain regions (cerebellum, hypothalamus, striatum, hippocampus and cortex) in the presence of different organic solvents normally used in the in vitro assay. The organic solvents used were acetone (C3H6O), acetonitrile (C2H3N), ethyl alcohol (C2H6O), isopropyl alcohol (C3H8O), methyl alcohol (CH4O), tert-butyl alcohol (C4H10O) and dimethyl sulfoxide (DMSO, C2H6OS) ranging from 0.6 to 10%. Ethyl and methyl alcohol presented no effect on AChE activity at any of the concentrations and brain structures tested. In the hippocampus, isopropyl alcohol did not demonstrate a significant inhibitory effect, even at high concentrations. Tert-butyl alcohol presented an interesting result, increased AChE activity (P < .05) in the hypothalamus (1.8%), cortex (1.8 and 2.5) and striatum (1.2, 1.8 and 2.5%) and decreased activity at a concentration of 10% in the cortex (P < .05) and striatum (P < .01). Acetone and acetonitrile presented similar results, both significantly inhibiting AChE in all structures (5%, P < .05 and 10%, P < .01). DMSO exhibited a highly inhibitory effect at practically all concentrations tested (P < .01). In conclusion, for testing new compounds on AChE activity in vitro, methyl and ethyl alcohol may be the best organic solvent choice.

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Section: Discussionmentioning

confidence: 98%

Effects per se of Organic Solvents in the Cerebral Acetylcholinesterase of Rats

et al. 2005

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“…AChE activity was determined by the method of Ellman et al (1961), modified as described by Pereira et al (2004). The mixture assay contained 1.04 mM DTNB, 24 mM potassium phosphate buffer pH 7.2 and 25 μL of enzymatic material.…”

Section: Methodsmentioning

confidence: 99%

ZnCl₂ exposure protects against behavioral and acetylcholinesterase changes induced by HgCl₂

Franciscato

Goulart

Lovatto

et al. 2009

Intl J of Devlp Neuroscience

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This study examined the effects of inorganic mercury exposure on behavioral and biochemical parameters and investigated the possible preventive effects of zinc on the alterations induced by mercury. Pups were exposed from 3rd to 7th postnatal day to ZnCl2 (27 mg/kg/day, s.c.) and subsequently to HgCl2 (5 doses of 5 mg/kg/day, s.c.). Each litter contained two rats for each treatment. The rats were submitted to behavioral task and litters were killed at 13 or 33 days old for acetylcholinesterase activity assays and for the determination of metal levels. Based on the results obtained from 13-day-old rats, they were divided in two groups of litters that were defined at the end of the experimental period (33 days) as less sensitive rats to mercury and more sensitive rats to mercury in accordance with the recovery of body weight until day 33. The mercury exposure caused accumulation of this metal in cerebrum and cerebellum in all mercury treated rats, and inhibited the cerebellum acetylcholinesterase activity from 13-day-old rats. Besides, the mercury-animals of the most sensitive litters to mercury presented impairment in motor function and muscular strength verified in the beaker test, as well as a reduction of the locomotor and exploratory activities in the open field task. Zinc partially prevented all the alterations induced by mercury exposure and reduced the mercury level accumulated in cerebrum and cerebellum. This study confirms the preventive effect of zinc on behavioral alterations induced by mercury in young rats and demonstrates that the mercury behavioral effects are present even for a long time after the end of the exposure.

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“…The colorimetric methodology used was previously described by Ellman, Courtney, Andres, and Featherstone (1961) and modified by Pereira, Adams, and Silva (2004). The assay medium contained a final concentration of 1.05 mM DTNB − 5′,5-dithiobis (2-nitrobenzoic acid), 24 mM potassium phosphate buffer (pH = 7.5), and 75 µL of supernatant solution (S1).…”

Section: Ache Activity Assaymentioning

confidence: 99%

Influence of nanoencapsulated lutein on acetylcholinesterase activity: In vitro determination, kinetic parameters, and in silico docking simulations

et al. 2020

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A B S T R A C TLutein is a bioactive found in dark leafy vegetables that may be used as a nutraceutical agent in foodstuff and an inhibitor of key enzymes of the human body such as those involved in the cholinergic system. However, its high hydrophobicity leads to low bioavailability and must be overcome if lutein is to be added in foods. The objective of this study was to evaluate the influence of nanoencapsulated lutein in the activity of the acetylcholinesterase enzyme. The in vitro study was carried out using water in order to evaluate the impact of encapsulation on the hydrophilicity of lutein. In vitro assays showed that lutein, both free and nanoencapsulated, presented a mixedtype inhibition behavior, and encapsulated lutein was able to inhibit acetylcholinesterase activity even in an aqueous medium. Inhibition was also showed by the in silico docking results which show that lutein interacted with the pocket region of the enzyme. that the damage to the cholinergic system is closely related to brain dysfunctions such as affective disorders, depression, schizophrenia and https://doi.

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2,5-Hexanedione inhibits rat brain acetylcholinesterase activity in vitro

Cited by 32 publications

References 27 publications

Effects per se of Organic Solvents in the Cerebral Acetylcholinesterase of Rats

Effects per se of Organic Solvents in the Cerebral Acetylcholinesterase of Rats

ZnCl₂ exposure protects against behavioral and acetylcholinesterase changes induced by HgCl₂

Influence of nanoencapsulated lutein on acetylcholinesterase activity: In vitro determination, kinetic parameters, and in silico docking simulations

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2,5-Hexanedione inhibits rat brain acetylcholinesterase activity in vitro

Cited by 32 publications

References 27 publications

Effects per se of Organic Solvents in the Cerebral Acetylcholinesterase of Rats

Effects per se of Organic Solvents in the Cerebral Acetylcholinesterase of Rats

ZnCl2 exposure protects against behavioral and acetylcholinesterase changes induced by HgCl2

Influence of nanoencapsulated lutein on acetylcholinesterase activity: In vitro determination, kinetic parameters, and in silico docking simulations

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ZnCl₂ exposure protects against behavioral and acetylcholinesterase changes induced by HgCl₂