The present study evaluated the incorporation of 5, 7.5, and 10% of Riesling skin flour (RSF) and Tannat skin flour (TSF) as wheat flour replacement in muffins. The flour was evaluated for gluten content and Alveograph parameters. The finished products were evaluated for chemical composition and technological properties, and a sensory analysis was performed. All samples presented higher dietary fiber content than wheat flour, and this was mainly in the form of soluble dietary fiber. The inclusion of grape skin flour decreased the lightness of the flour and increased the lightness values (L*) of the muffin crumbs and crusts. The hardness of the muffins that incorporated RSF and TSF muffins increased as the percentage of the skin flour increased. There was a decrease in the cohesiveness value as the skin flour percentage increased. Consumers did not perceive the levels of substitution. The results of the sensory analysis indicated that grape skin can be added to the ingredients of muffins without changing the color, taste, flavor, texture, and overall acceptability of the final product.

This study examined the effects of inorganic mercury exposure on behavioral and biochemical parameters and investigated the possible preventive effects of zinc on the alterations induced by mercury. Pups were exposed from 3rd to 7th postnatal day to ZnCl2 (27 mg/kg/day, s.c.) and subsequently to HgCl2 (5 doses of 5 mg/kg/day, s.c.). Each litter contained two rats for each treatment. The rats were submitted to behavioral task and litters were killed at 13 or 33 days old for acetylcholinesterase activity assays and for the determination of metal levels. Based on the results obtained from 13-day-old rats, they were divided in two groups of litters that were defined at the end of the experimental period (33 days) as less sensitive rats to mercury and more sensitive rats to mercury in accordance with the recovery of body weight until day 33. The mercury exposure caused accumulation of this metal in cerebrum and cerebellum in all mercury treated rats, and inhibited the cerebellum acetylcholinesterase activity from 13-day-old rats. Besides, the mercury-animals of the most sensitive litters to mercury presented impairment in motor function and muscular strength verified in the beaker test, as well as a reduction of the locomotor and exploratory activities in the open field task. Zinc partially prevented all the alterations induced by mercury exposure and reduced the mercury level accumulated in cerebrum and cerebellum. This study confirms the preventive effect of zinc on behavioral alterations induced by mercury in young rats and demonstrates that the mercury behavioral effects are present even for a long time after the end of the exposure.

No abstract

The purpose of this study was to develop and improve protein concentration techniques for two industrial by-products with the potential for use in fish feed. In particular, we chemically characterized crambe meal and sunflower meal and their protein concentrates. Three different protein concentration methods were tested: isoelectric pH (pHi), acid pH and alkaline pH. For crambe and sunflower meals extraction using the pHi method was most efficient in terms of protein yield and crude protein content in the concentrates; this method also increased lysine and methionine content in the concentrates. The water holding capacity of the sunflower protein concentrate was greater than that of the crambe protein concentrate. The crambe protein concentrate had a foam-formation capacity of 15%, which stabilized at 6% after 90 minutes. The protein concentration method also reduced total phenolic content by approximately 50% in the concentrates compared with the meals. Therefore, we conclude that protein concentration using the pHi method is the most efficient technique for crambe and sunflower meals, and the use of this technique can decrease total phenolic compounds while improving meal quality for fish feeding.

This study was conducted out to evaluate the effect of partial replacement of animal protein (PBOA) for crude protein demucilaged linseed meal (PBFLD) and in nature (PBFL) ± 1.85 g) were reared in water recirculation system and fed to apparent satiation three times a day. At the end experimental parameters were evaluated for growth, carcass, chemical composition in whole fish and digestive enzymes. The fish fed control diet had best levels of feed conversion (p <0.05), however the remainder of the growth parameters were not altered by the inclusion of FL and FLD. There were no significant differences in hepatosomatic relation (RHS) and digestivosomatic relation (RDS). Diet 35%FLD had a lower intestinal quotient (QI), moisture content and higher carcass fat, total fat deposited and activity of the enzyme trypsin. It can be concluded that the FLD and FL can be used as a substitute of meat meal to the diets in juvenile jundia feed as an alternative source and cost.

ResumoO objetivo do estudo foi avaliar o efeito da utilização de concentrados proteicos de farelos de girassol e crambe na dieta de juvenis de jundiá, como substitutos da fonte proteica de origem animal. Foram utilizados 300 jundiás criados em 15 unidades experimentais de 280 L, constituindo cinco tratamentos com três repetições. Foram avaliados dois níveis (25 e 50%) de substituição da proteína da farinha de carne e ossos suína pelos concentrados proteicos de farelos de girassol e crambe. Avaliaramse parâmetros de crescimento, índices zootécnicos e enzimas digestivas nos peixes. Não houve diferença estatística para massa (g) e comprimento padrão (cm), mas os peixes da dieta CPFCr-25% apresentaram maior comprimento total (cm). Na carcaça, não houve diferença no teor de matéria seca, proteína bruta e proteína total depositada (calculada). Contudo, observou-se maior concentração de matéria mineral na carcaça dos animais alimentados com as dietas controle e CPFCr-50% em relação à dieta CPFG-50%, além do maior teor de lipídeos nos peixes que receberam a dieta CPFG-50% em relação aos demais grupos. Não houve diferenças significativas para índice hepatossomático, índice digestivossomático e quociente intestinal dos animais submetidos aos distintos tratamentos. A atividade das enzimas digestivas tripsina e quimotripsina não apresentaram alteração, porém verificou-se aumento da atividade da protease ácida. O aumento quantitativo e qualitativo de proteína a partir da concentração desta fração permite a utilização dos concentrados proteicos de farelos de girassol e crambe como substitutos à fonte proteica de origem animal. Palavras-chave: Farelos vegetais, concentração proteica, alimentação de peixes, farelo de girassol, farelo de crambe AbstractThe purpose of study was to evaluate the effect of using protein concentrates crambe and sunflower meal in the diet of silver catfish juveniles, as substitute for animal protein source. A total of 300

The aim of this study was to assess the effects of different dietary fibre concentrates (DFC: Mucilage = MG; pectin = PN or β-glucan+mannan = βg+M), on biochemical parameters, stress and immune response and skin mucus of jundiá (Rhamdia quelen). The fish (7.16 ± 0.06 g) were fed with Control diet (0 g/kg of DFC); diet supplemented with 5 g/kg of commercial prebiotic (CP 5) or diets supplemented with 5 or 10 g/kg of MG; PN or βg+M. After 8 weeks of the feeding trials, biochemical parameters (cholesterol, glucose, albumin and total protein), cortisol, immunoglobulin IgM and mucoproteins of skin mucus were assessed. Results demonstrated that supplementation with PN increased cholesterol levels (p<.05). After application of the stressor, most fish, except those in the PN and 10 g/kg MG groups, showed significant increases (p<.05) in cholesterol, glucose and albumin levels. The jundiás showed no difference in cortisol levels after application of the stressor (p>.05). IgM levels were significantly high in fish supplemented with DFC (p<.05). However, the concentration of mucoproteins in skin mucus was not influenced in the different treatments (p>.05). The results showed that supplementation with DFC promoted beneficial effects on the metabolism of jundiá. K E Y W O R D Sfibre dietary, mucilage, pectin, prebiotics, Rhamdia quelen, β-glucan+mannan

A study was conducted to investigate the effect of Dietary Fiber Concentrates (DFCs) on growth performance, gut morphology, and hepatic metabolic intermediates in jundiá (Rhamdia quelen). At the end of the trial, growth and intestinal villus height was significantly (P< 0.05) higher in fish fed diets supplemented with DFCs. However, the animals in commercial prebiotic group showed higher values for this variable compared to the other treatments. Regarding the thickness of the epithelium bowel, it was greater in the Control group compared to animals supplemented with β-glucan+mannan. Likewise, treatment with commercial prebiotic showed higher values of epithelium bowel compared to the DFCs. The fish supplemented with DFCs, had higher glycogen storage compared to the control group. These results indicate that DFCs can be considered as a beneficial dietary supplement for improving growth performance, gut morphology, and hepatic metabolic intermediates of jundiá.