2,4-Dimetilpirol’ün Başak Yanıklığı Hastalığı Etmeni Fusarium culmorum’un Üzerine Etkilerinin İncelenmesi

Abstract

Özet: Bitki patojeni Fusarium culmorum dünyada ve ülkemizde tahıllar üzerinde kök çürüklüğü ve başak yanıklığı başta olmak üzere çeşitli hastalıklara neden olmaktadır. Bu çalışmada F. culmorum ile mücadele kullanılabilecek yeni ve potansiyel bir ajan olarak 2,4-Dimetilpirol'ün patojen üzerindeki etkileri incelenmiştir. Bu amaçla farklı konsantrasyonlarda 2,4-Dimetilpirol (0, 0.5, 1, 2 ve 4 mg mL -1 ) uygulanmış F. culmorum 20F izolatında artan konsantrasyonlarda doğrusal büyüme oranındaki (DBO) değişim incelenmiş ve kontrol ve deney grupları arasında anlamlı bir düşüş olduğu görülmüştür. Ayrıca seksüel üreme ve hücre çeperi bütünlüğünden sorumlu Mgv1 geninin anlatımı gerçek zamanlı kantitatif polimeraz zincir reaksiyonu (k-PZR) ve ters transkripsiyon PZR (RT-PZR) ile incelenmiştir. Deney gruplarında kontrol gruplarına göre Mgv1 geni için 5.21±0.05x10 2 kat artma saptanmıştır. Elde edilen bulgulara göre 2,4-Dimetilpirol'ün, F. culmorum türünde potansiyel bir antifungal etkili ajan olabileceği gösterilmiştir. İleriki çalışmalarda bu ajanın tarladaki fungal biyokütlenin ve toksin üretiminin azaltılmasını sağlayarak hastalık ile mücadelede yeni bir yaklaşım olarak kullanılabileceği düşünülmektedir. Anahtar Kelimeler: Antifungal; Başak Yanıklığı; Fusarium; 2,4-Dimetilpirol Investigation of the Effects of 2,4-Dimethylpyrrole on Fusarium culmorum Causal Agent of Head Blight DiseaseReceived: 31 January 2017 -Revised: 11 April 2017 -Accepted: 19 April 2017 Abstract: Fusarium culmorum, a phytopathogen, cause several diseases including root rot and head blight on cereals in worldwide and our country. In this study, effects of 2,4-Dimethylpyrrole, as a novel and potential agent on pathogen which can be used in fight with F. culmorum were investigated. For this purpose, alteration in linear growth rate (LGR) in F. culmorum 20F isolate, subjected to different concentrations of 2,4-Dimethylpyrrole (0, 0.5, 1, 2, 4 mg mL -1

Tetraconazole Leads to Alterations in Fusarium Graminearum at Different Molecular Levels

“…These genes or homologues have been used in different studies related to the apoptosis mechanism, downregulation of mycotoxin synthesis and the novel antifungal resistance etc. (Ponts et al, 2007;Sharon et al, 2009;Shlezinger, Goldfinger and Sharon, 2012;Sefer et al, 2017;Yörük et al, 2018b). However, in this study, it was claimed that the alterations in Hog1, Mgv1, POD and tri5 genes could be used as markers in revealing the detailed antifungal effects of any specific chemical compound.…”

“…It can be easily said that this level of fungicide could provide a powerful strategy to overcome the F. graminearum infections in the fields. Especially, if and when the MIC value is compared to the potential antifungal agents, the MIC values which could reach up to 1 mg/mL (Sefer et al, 2017;Yörük et al, 2018a), TCZ could be considered as a strong antimicrobial agent.…”

Investigation of Camphor Effects on Fusarium graminearum and F. culmorum at Different Molecular Levels

“…So far, the antifungal potential of some chemical compounds derived from plant or bacteria have been investigated in Fusarium species. However, comprehensive data were not obtained from those studies based on the investigation of the macroconidium reproduction capacity, radial growth rate, and inhibition of mycotoxin biosynthesis alterations [17,18,25,26]. Gene alterations, which are related to important processes in the fungal life cycle, the detection of apoptosis-like processes, and the determination of the presence of oxidative stress, have not been investigated in F. graminearum and F. culmorum .…”

“…In this study, the MIC values for these two species were found at moderate levels as compared with some other plant essential oils such as eugenol, vanillin, dimethyl pyrrole, etc. In addition, a relatively higher level of mycotoxin inhibition was also detected via qPCR [17,18,26,27,28,29,30]. IC 50 values of 1 µg/µL were used in further studies.…”

“…The major components of essential oils obtained from plant extracts (kapyrrole derivatives, indole derivatives, etc.) have been used to inhibit fungal growth [17,18,19]. One of the major components of essential oils, camphor (C 10 H 16 O), is a bicyclic monoterpene ketone widely found in several aromatic plants such as Cinnamonum camphora , Piper capense , and Salvia officinalis .…”