α-Thujone exhibits an antifungal activity against F. graminearum by inducing oxidative stress, apoptosis, epigenetics alterations and reduced toxin synthesis

Teker, Tuğba; Sefer, Özlem; Gazdağlı, Aylin; Yörük, Emre; Varol, Gülin İnci; Albayrak, Gülruh

doi:10.1007/s10658-021-02269-w

Cited by 9 publications

(15 citation statements)

References 55 publications

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“…[ 52 , 53 , 54 , 55 , 56 ]. Cis -thujone, a relevant compound in this essential oil, also exerted antimicrobial activity [ 57 , 58 ]. On the other hand, weak antimicrobial activity was reported for the camphor [ 59 , 60 , 61 ].…”

Section: Discussionmentioning

confidence: 99%

Advances in the Phytochemical Characterisation and Bioactivities of Salvia aurea L. Essential Oil

Alves-Silva

Maccioni

Cocco

et al. 2023

Plants

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The Salvia L. genus (Lamiaceae) is largely used in the pharmaceutical and food industry. Several species of biological relevance are extensively employed in traditional medicine, including Salvia aurea L. (syn. S. africana-lutea L.), which is used as a traditional skin disinfectant and in wounds as a healing remedy; nevertheless, these properties have not been validated yet. The aim of the present study is to characterise S. aurea essential oil (EO), unveiling its chemical composition and validating its biological properties. The EO was obtained by hydrodistillation and subsequently analysed by GC-FID and GC-MS. Different biological activities were assessed: the antifungal effect on dermatophytes and yeasts and the anti-inflammatory potential by evaluating nitric oxide (NO) production and COX-2 and iNOS protein levels. Wound-healing properties were assessed using the scratch-healing test, and the anti-aging capacity was estimated through the senescence-associated beta-galactosidase activity. S. aurea EO is mainly characterised by 1,8-cineole (16.7%), β-pinene (11.9%), cis-thujone (10.5%), camphor (9.5%), and (E)-caryophyllene (9.3%). The results showed an effective inhibition of the growth of dermatophytes. Furthermore, it significantly reduced protein levels of iNOS/COX-2 and simultaneously NO release. Additionally, the EO exhibited anti-senescence potential and enhanced wound healing. Overall, this study highlights the remarkable pharmacological properties of Salvia aurea EO, which should be further explored in order to develop innovative, sustainable, and environmentally friendly skin products.

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Section: Discussionmentioning

confidence: 99%

Advances in the Phytochemical Characterisation and Bioactivities of Salvia aurea L. Essential Oil

Alves-Silva

Maccioni

Cocco

et al. 2023

Plants

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“…We found that methanol extract induced antioxidant mechanism and apoptosis while suppressing trichothecene biosynthesis. Similarly, there are some reports of increased cat and mst20 levels and decreased tri5 level in determining the antifungal activity of the compounds (Gazdağlı et al, 2018;Shao et al, 2021;Teker et al, 2021;Xiu et al, 2021;Yörük et al, 2022).…”

Section: Discussionmentioning

confidence: 84%

Investigation of the antifungal activity of lichen (Usnea longissima) extracts against Fusarium graminearum

TAGHİYEVA

Dülger

Yörük

et al. 2022

Anatolian Journal of Botany

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The aim of this study was to investigate the antifungal effects and molecular changes caused by Usnea longissima Ach. extracts against Fusarium greaminearum. In agar well diffusion assay, the zone of inhibition increased as the concentration increased in both of methanol and acetone extracts (1, 10, 20 and 50 mg/ml). In terms of bioactivities, 1 mg/ml was active, while other concentrations were very active. At the molecular level, changes caused by 50 mg/ml methanol extract was analyzed by qPCR with terms of cat, mst20, and tri5 genes, which are associated with antioxidation, apoptosis, and trichothecene production, respectively. Transcript levels of tri5 decreased (0.29 fold) while cat (2.41 fold) and mst20 (1.48 fold) increased. Findings from this study showed that U. longissima extracts could be natural antifungal agent against worldwide phytopathogen F. graminearum.

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“…In addition, β-pinene and 1,8-cineole (present in significant amounts in both sage and bay laurel essential oils) were positively correlated with the inhibition of S. parasitica zoospore germination, explaining the similar EC 50 values of these two essential oils for S. parasitica zoospore germination (EC 50 0.012 and 0.013 µL/mL, respectively). Some of these compounds were previously reported to exhibit good anti-oomycete [ 44 , 55 ] and antifungal [ 56 , 57 , 58 , 59 ] activity. For example, camphor (up to 38.06 µg/mL) progressively slowed down the mycelial growth of S. parasitica and S. delica , while thujone and β-pinene (500 and 1000 µg/mL, respectively) inhibited the mycelial growth of S. parasitica [ 44 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

“…For example, camphor (up to 38.06 µg/mL) progressively slowed down the mycelial growth of S. parasitica and S. delica , while thujone and β-pinene (500 and 1000 µg/mL, respectively) inhibited the mycelial growth of S. parasitica [ 44 , 55 ]. Moreover, α-thujone and camphor have potent antifungal activity against Fusarium graminearum , F. culmorum , and Schizosaccharomyces pombe , which is mainly explained by the induction of oxidative stress and subsequent apoptotic cell death, but also by a decrease in genomic stability and epigenetic changes [ 56 , 57 , 58 , 59 ]. Thus, the high camphor content in sage essential oil probably contributed significantly to the observed inhibitory effects.…”

Section: Discussionmentioning

confidence: 99%

Essential Oils of Sage, Rosemary, and Bay Laurel Inhibit the Life Stages of Oomycete Pathogens Important in Aquaculture

Miljanović

Grbin

Pavić

et al. 2021

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Saprolegnia parasitica, the causative agent of saprolegniosis in fish, and Aphanomyces astaci, the causative agent of crayfish plague, are oomycete pathogens that cause economic losses in aquaculture. Since toxic chemicals are currently used to control them, we aimed to investigate their inhibition by essential oils of sage, rosemary, and bay laurel as environmentally acceptable alternatives. Gas Chromatography–Mass Spectrometry (GC–MS) analysis showed that the essential oils tested were rich in bioactive volatiles, mainly monoterpenes. Mycelium and zoospores of A. astaci were more sensitive compared to those of S. parasitica, where only sage essential oil completely inhibited mycelial growth. EC50 values (i.e., concentrations of samples at which the growth was inhibited by 50%) for mycelial growth determined by the radial growth inhibition assay were 0.031–0.098 µL/mL for A. astaci and 0.040 µL/mL for S. parasitica. EC50 values determined by the zoospore germination inhibition assay were 0.007–0.049 µL/mL for A. astaci and 0.012–0.063 µL/mL for S. parasitica. The observed inhibition, most pronounced for sage essential oil, could be partly due to dominant constituents of the essential oils, such as camphor, but more likely resulted from a synergistic effect of multiple compounds. Our results may serve as a basis for in vivo experiments and the development of environmentally friendly methods to control oomycete pathogens in aquaculture.

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α-Thujone exhibits an antifungal activity against F. graminearum by inducing oxidative stress, apoptosis, epigenetics alterations and reduced toxin synthesis

Cited by 9 publications

References 55 publications

Advances in the Phytochemical Characterisation and Bioactivities of Salvia aurea L. Essential Oil

Advances in the Phytochemical Characterisation and Bioactivities of Salvia aurea L. Essential Oil

Investigation of the antifungal activity of lichen (Usnea longissima) extracts against Fusarium graminearum

Essential Oils of Sage, Rosemary, and Bay Laurel Inhibit the Life Stages of Oomycete Pathogens Important in Aquaculture

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