1H NMR metabolic phenotyping of Dipterocarpus alatus as a novel tool for age and growth determination

Abstract

Dipterocarpus alatus belongs to Family Dipterocarpaceae that can be commonly found in Southeast Asian countries. It is a perennial plant with oval-shaped leaves and oleoresin-rich wood. It has been considered as a multipurpose plant since all parts can be practically utilized. One of the major problems for utilizing Dipterocarpus alatus is the difficulty knowing the exact age as this kind of plant is ready for multipurpose use after 20 years of age. At present, the most commonly used method for determining age of Dipterocarpus alatus is the annual ring estimation. However, this conventional method is unable to provide the high precision and accuracy of age determination due to its limitation including blurry annual rings caused by enriched oleoresin in the wood. The current study aimed to investigate the differences of 1H -NMR spectroscopy-based metabolic profiles from bark and leaf of Dipterocarpus alatus at different ages including 2, 7, 15 and 25 years. Our findings demonstrated that there is a total of 56 metabolites shared between bark and leaf. It is noticeable that bark at different ages exhibited the strongest variation and sugar or sugar derivatives that were found in higher concentrations in bark compared with those in leaf. We found that decreasing levels of certain metabolites including tagatose, 1’kestose and 2’-fucosyllactose exhibited the promising patterns. In conclusion, panel metabolites involved in the sucrose biosynthesis can precisely determine the age and growth of Dipterocarpus alatus.

Rats Orally Administered with Ethyl Alcohol for a Prolonged Time Show Histopathology of the Epididymis and Seminal Vesicle Together with Changes in the Luminal Metabolite Composition

“…Statistical total correlation spectroscopy (STOCSY) was used to verify the appearances of correlated resonances on one-dimensitional NMR spectra [50], which were searched against public databases including the human metabolome database (HMDB) and ChenomxNMR Suite version 9.0 (Chenomx, Inc., Edmonton, AB, Canada) [51][52][53][54]. To semiquantify the metabolites in each fluid, the maximum intensity obtained from the individual peaks identified in the 1 H NMR spectra was calculated for the concentration by referencing to the known TSP concentration [55]. A metabolic profiling experiment of all fluid samples was conducted at Khon Kaen University Phenome Center, Khon Kaen, Thailand.…”

Prolonged Oral Administration of Ethyl Alcohol Leads to Histopathology of the Epididymis and Seminal Vesicle and Changes of Metabolite Composition in the Tissue Lumen

“…Then, identified metabolites were confirmed with the online database, Chenomx NMR Suite software (Chenomx, Inc., Edmonton, Alberta, Canada) and Human Metabolome Database (HMDB, https://hmdb.ca), based on the correlation of their specific chemical shifts. To quantify the metabolites in each fluid, the maximum intensity obtained from individual peaks identified in 1 H NMR spectra was calculated for the concentration by referencing to the known TSP concentration [50]. Because the fluid sample was diluted twofold with the NMR buffer for the analysis, the obtained concentration of each metabolite was multiplied by 2 to obtain its concentration in CEF and SVF.…”

Recent Advances in Nanozyme-Mediated Strategies for Pathogen Detection and Control

“…To overcome the limitations of conventional ELISA techniques, such as colorimetric reactions, substantial sample volume requirements (approximately 200 µL), extended processing time (1-2 h), and the need for bulky instrumentation to interpret test results [86], the utilization of paper-based enzyme-linked immunosorbent assay (P-ELISA) tests has emerged as a promising solution to address these challenges, particularly in point-of-care testing (POCT) settings [87]. P-ELISA tests offer additional advantages, such as cost effectiveness and ease of use.…”