1H MAS and 1H ? 31P CP/MAS NMR Study of Human Bone Mineral

Kaflak-Hachulska, A; Samoson, Ago; Kołodziejski, Wacław

doi:10.1007/s00223-002-2111-5

Cited by 114 publications

(181 citation statements)

References 49 publications

Supporting

Mentioning

159

Contrasting

Order By: Relevance

“…In the literature, [1,24,27,32] there is an incessant discussion on the presence of the HPO 4 2− ions in apatites. Hence, at the very beginning, it will be enlightening to examine results for BRU and HAc (Table 2).…”

Section: Discussionmentioning

confidence: 99%

“…[24,34,35,48] Stoichiometric HA was synthesized by the wet chemical method with the Ca/P ratio of 1.67. [49] It was predried in air to give as-synthesized sample HAh.…”

Section: Methodsmentioning

confidence: 99%

“…The latter parameter reflects the 1 H-31 P dipolar couplings [51] and in the former publications [1,24,34,44,51,52] it has been designated by T 2 . For a rigid lattice λ = (n + 1) −1 , where n is the number of protons in close neighborhood of the observed 31 P nucleus.…”

Section: Theorymentioning

confidence: 99%

“…The HPO 4 2− ions of BRU form strong hydrogen bonds with structural water and therefore give a proton peak at 10.4 ppm. [1,24] The content of HPO 4 2− ions in the bone mineral decreases and that of CO 3 2− increases with aging. [15,27,28] The nanocrystallinity of the bone apatite is closely related to physiological functions of bone.…”

Section: Introductionmentioning

confidence: 99%

“…[1,23] However, it is not directly observed in the 1 H magic angle spinning (MAS) NMR spectrum of bone. [1,24] The bone apatite contains 5-8 wt% of CO 3 2− ions substituted for PO 4 3− ions (substitution of type B). [6,8,10,25] Therefore, a useful reference mineral to examine would be calcium carbonatoapatite of type B (CHA-B).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Kinetics of ¹H → ³¹P NMR cross‐polarization in bone apatite and its mineral standards

Kaflak

Kołodziejski

2008

Magnetic Reson in Chemistry

View full text Add to dashboard Cite

Kinetics of NMR cross-polarization (CP) from protons to phosphorus-31 nuclei was studied in the following samples: mineral of whole human bone, apatite prepared from bone, natural brushite, synthetic hydroxyapatite (hydrated and calcined), and synthetic carbonatoapatite of type B with 9 wt% of CO(3) (2-). In order to avoid an effect of magic angle spinning on CP and relaxation, the experiments were carried out on static samples. Parameters of the CP kinetics were discussed for trabecular and cortical bone tissue from adult subjects in comparison to the synthetic mineral standards. It was found that carbonatoapatite shows similar CP behavior to the bone mineral. Both materials undergo two-component CP kinetics. The fast-relaxing classical component is from the surface of apatite crystals and the slow-relaxing nonclassical component comes from the crystal interior. The components have been unambiguously assigned using inverse CP from phosphorus-31 to protons. The study provides information on a structured water layer, which covers crystal surface of carbonato- and bone apatite. The layer encompasses ca 40% of apatite phosphorus and its thickness is more than ca 2 nm.

show abstract

Section: Discussionmentioning

confidence: 99%

“…[24,34,35,48] Stoichiometric HA was synthesized by the wet chemical method with the Ca/P ratio of 1.67. [49] It was predried in air to give as-synthesized sample HAh.…”

Section: Methodsmentioning

confidence: 99%

Section: Theorymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Kinetics of ¹H → ³¹P NMR cross‐polarization in bone apatite and its mineral standards

Kaflak

Kołodziejski

2008

Magnetic Reson in Chemistry

View full text Add to dashboard Cite

show abstract

Periosteum Extracellular‐Matrix‐Mediated Acellular Mineralization during Bone Formation

Lin

Zhao

Zhu

et al. 2017

Adv Healthcare Materials

View full text Add to dashboard Cite

Cell-mediated mineralization is essential for bone formation and regeneration. In this study, it is proven that extracellular matrix (ECM) of decellularized periosteum can play an initiative and independent role in bone-like apatite formation. Using decellularized periosteum scaffold, it is revealed that ECM scaffold itself can promote critical bone defect regeneration and nude mouse ectopic ossification. The natural collagen matrix of decellularized periosteum can serve as a 3D structural template for Ca-P nuclei initiation, controlling the size and orientation of bone-like mineral crystals. The naturally cross-linked and highly ordered 3D fibrillar network of decellularized periosteum not only provides a model for mimicking mineralization in vitro and in vivo to elucidate the important functions of ECM in bone formation and regeneration, but also can be a promising biomaterial for bone tissue engineering and clinical application.

show abstract

Glucose‐lowering Activity of Amino Acid‐N‐phosphonic Acid Oxovanadium Complexes and Its Interaction with DNA

Liu

Sheng-di

et al. 2006

Chin. J. Chem.

View full text Add to dashboard Cite

Vanadium has well-documented lowering glucose properties both in vitro and in vivo. The design of new oxovanadium(IV) coordination compounds, intended for use as insulin-enhancing agents in the treatment of diabetes mellitus, can potentially benefit from a synergistic approach, in which the whole complex has more than an additive effect from its component parts. Biological testing with oxovanadium(IV) organic phosphonic acid, for insulin-enhancing potential included acute administration, by oral gavage in streptozotocin (STZ) diabetic rats. The complexes of oxovanadium(IV) amino acid-N-phosphonic acid exhibit higher lowering glucose activity in vivo. The interaction of the complexes of oxovanadium(IV) amino acid-N-phosphonic acid with DNA was investigated by agarose gel electrophoresis. The results indicated that these complexes have strong interaction with DNA.Keywords oxovanadium, amino acid-N-phosphonic acid, lowering glucose activity, interaction IntroductionDiabetes mellitus is a heterogeneous disorder. It is characterized by hyperglycemia, alterations in carbohydrate and lipid metabolism, and vascular and neurological complications. An underlying insufficiency of insulin results from either inadequate pancreatic production, 1,2 or defective insulin utilization. 3 Good diabetic control with available therapies continues to be an elusive goal. Thus, there is a need to find effective, orally active drugs that mimic or enhance the properties of insulin.Vanadium, a trace element in the environment, is found in plants and animals, and is also found as a vanadium enzyme, vanadium bromoperoxidase, in marine macroalgae, and thus the role of vanadium in biological functions has been extensively investigated. 4,5 The finding in 1980 that vanadate(V) has an insulin-mimetic effect, and studies indicating that vanadium directly affects glucose metabolism, have stimulated research on insulin-mimetic vanadium compounds. [6][7][8][9][10][11][12][13] Oral administration of insulin in mammals is ineffective; therefore, diabetic patients receive insulin by subcutaneous injection. The availability of orally administered insulin substitutes could be of importance in the treatment of diabetics. Experimental Chemicals and instrumentationAll solvents and chemicals were of reagent grade and used without further purification unless otherwise specified: glycine-N-methene phosphonic acid, alanine-N-methenephosphonic acid, vanadyl sulfate trihydrate. Water was deionized. The yields are for analytically pure compounds and calculations are based on vanadium. All complexation reactions were carried out under nitrogen.The complexes were characterized by IR spectra, elemental analyses, 31 P MAS NMR and 51 V NMR susceptibility. Elemental analyses were performed on a Perkin Elmer 2400 CHN Elemental analyzer. P and V were determined by PLASMA-SPEC (I) inductively coupled plasma spectrometer. Infrared spectra were recorded as KBr disks in the range 4000-400 cm -1 on a Magna FT-IR 560 spectrometer.51 V NMR spectra were obtained on a Varian Unity-...

show abstract

1H MAS and 1H ? 31P CP/MAS NMR Study of Human Bone Mineral

Cited by 114 publications

References 49 publications

Kinetics of ¹H → ³¹P NMR cross‐polarization in bone apatite and its mineral standards

Kinetics of ¹H → ³¹P NMR cross‐polarization in bone apatite and its mineral standards

Periosteum Extracellular‐Matrix‐Mediated Acellular Mineralization during Bone Formation

Glucose‐lowering Activity of Amino Acid‐N‐phosphonic Acid Oxovanadium Complexes and Its Interaction with DNA

Contact Info

Product

Resources

About

1H MAS and 1H ? 31P CP/MAS NMR Study of Human Bone Mineral

Cited by 114 publications

References 49 publications

Kinetics of 1H → 31P NMR cross‐polarization in bone apatite and its mineral standards

Kinetics of 1H → 31P NMR cross‐polarization in bone apatite and its mineral standards

Periosteum Extracellular‐Matrix‐Mediated Acellular Mineralization during Bone Formation

Glucose‐lowering Activity of Amino Acid‐N‐phosphonic Acid Oxovanadium Complexes and Its Interaction with DNA

Contact Info

Product

Resources

About

Kinetics of ¹H → ³¹P NMR cross‐polarization in bone apatite and its mineral standards

Kinetics of ¹H → ³¹P NMR cross‐polarization in bone apatite and its mineral standards