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Nair, Lakshmi G.; Seeni, S.

doi:10.1023/a:1025001214995

Cited by 27 publications

(6 citation statements)

References 5 publications

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“…Comparing the multiplication results with those obtained in the culture of nodal segments of C. apetalum, where the maximum number of shoots per explant was 5.3 after 5 weeks, even with the use of 13 µM BA (Nair and Seeni, 2003), we conclude that the rate of multiplication of guanandi segments is satisfactory (mean rate of 4.8 per month and up to 18 shoots per explant after 12 weeks). However, very young shoots (< 2 cm) should remain longer in multiplication medium, or transferred to a PGR-free medium to elongate, because they cannot be separated and transferred to a rooting medium without suffering necrosis.…”

Section: Discussionmentioning

confidence: 54%

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Micropropagation of Calophyllum brasiliense (Cambess.) from nodal segments

et al. 2016

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Micropropagation of Calophyllum brasiliense Cambess. (Clusiaceae) is a way to overcome difficulties in achieving large-scale plant production, given the recalcitrant nature of the seeds, irregular fructification and absence of natural vegetative propagation of the species. Cultures were established using nodal segments 2 cm in length, obtained from 1-2 year old seedlings, maintained in a greenhouse. Mercury chloride and Plant Preservative Mixture™ were used in the surface sterilizing stage, better results being achieved with Plant Preservative Mixture™ incorporation in culture medium, at any concentration. Polyvinylpyrrolidone, activated charcoal, cysteine, ascorbic acid or citric acid were added to the culture medium to avoid oxidation. After 30 days of culture, polyvinylpirrolidone and ascorbic acid gave better results, eliminating oxidation in most explants. For shoot multiplication, benzylaminopurine was used in concentrations of 4.4 and 8.8 µM in Woody Plant Medium, resulting in an average of 4.43 and 4.68 shoots per explant, respectively, after 90 days. Indole-3-butyric acid and α-naphthalene acetic acid were used to induce root formation, reaching a maximum rooting rate of 24% with 20µM α-naphthalene acetic acid. For acclimatization. the rooted plants were transferred to Plantmax  substrate and cultured in a greenhouse, reaching 79% of survival after 30 days and 60% after one year.

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Section: Discussionmentioning

confidence: 54%

“…According to Mng'Omba et al (2012), HgCl 2 is stronger than NaOCl, which is the likely reason for its effectiveness in combating fungi, even endogenous species. The mortality of the cultures may be higher due to damage caused by stronger disinfectants, as was the case with Calophyllum apetalum (Nair and Seeni, 2003).…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of Calophyllum brasiliense (Cambess.) from nodal segments

et al. 2016

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“…These multiplication rates are close to those found for C. apetalum. For this species, the maximum number of shoots obtained from nodal segments of a mature tree was 3.2 after 6 weeks (Nair & Seeni, 2003). …”

Section: Shoot Multiplicationmentioning

confidence: 91%

“…Concentrations and exposure times for different chemicals varies depending on explants type (Leifert et al, 1991). A fungicide was used before disinfestation of C. inophyllum seeds (Thengane et al, 2006) and for the same species and C. apetalum, mercury chloride was applied to seeds (Nair & Seeni, 2003;Thengane et al, 2006), showing effective results.…”

Section: Introductionmentioning

confidence: 99%

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In vitro seed germination and multiplication of Calophyllum brasiliense

2016

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Calophyllum brasiliense is a tree species with limited natural reproduction. In vitro germination may be an alternative for obtaining high-quality seedlings. Seeds were maintained in water before surface disinfestation and compared with control seeds (i.e. not immersed), without differences between treatments. HgCl2 used during surface-disinfestation reduced contamination rates of cultures. Fungal contamination was reduced with fungicide added to culture medium (23 to 6.4%), although bacterial contamination increased (24 to 36%). In another experiment, seeds were immersed in plant preservative mixture (PPM™) prior to surface disinfestation. By combining immersion for 48 h and 2 mL L-1 in culture medium, contamination was only 6%. Seeds immersion in GA3 prior to surface disinfestation reduced root formation as concentration increased. Germination rate and GSI were reduced, respectively, from 72% and 0.129 (24 h) to 60% and 0.092 (48 h) according to exposure time to GA3. After 90 days in multiplication medium containing benzylaminopurine, average number of shoots per nodal segment was 3.4. In conclusion, in vitro germination of C. brasiliense seeds is feasible in sucrose-free WPM medium and reaches a high contamination-free rate (up to 93.3%).

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In Vitro Multiplication and Conservation of Threatened Medicinal Plants of Western Ghats of South India

Radha

2020

Conservation and Utilization of Threatened Medicinal Plants

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Untitled

Cited by 27 publications

References 5 publications

Micropropagation of Calophyllum brasiliense (Cambess.) from nodal segments

Micropropagation of Calophyllum brasiliense (Cambess.) from nodal segments

In vitro seed germination and multiplication of Calophyllum brasiliense

In Vitro Multiplication and Conservation of Threatened Medicinal Plants of Western Ghats of South India

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