14. Surface graph and densitometric SDS-PAGE studies in chickpea mutants

Kozgar, M. Imran; Wani, Mohd Rafiq; Khan, Sardar; Tomlekova, Nasya

doi:10.3920/978-90-8686-787-5_14

Cited by 2 publications

(4 citation statements)

References 9 publications

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“…Nonetheless, in conformity with the results reported by Laskar et al 27 on Lens, we were able to make use of SDS-PAGE as a tool for determining genetic relationships within a mutagenized population of Vigna unguiculata. The variations in the electrophoresis patterns of the seed proteins after mutagen treatments have also been reported in various pulse crops [35][36][37][38][39] . Hong et al 40 advocated that the response of mutants towards a wide range of environmental stresses can also be determined using SDS-PAGE analysis and would be useful in screening the stress tolerant mutants with high yield.…”

Section: Seed Protein and Mineral Element Contentsmentioning

confidence: 74%

“…Hong et al 40 advocated that the response of mutants towards a wide range of environmental stresses can also be determined using SDS-PAGE analysis and would be useful in screening the stress tolerant mutants with high yield. Belele et al 41 in Phaseolus vulgaris, Auti and Apparao 42 in Vigna radiata, Nakagawa et al 43 in Glycine max, Khadke and Kothekar 44 in Vigna acconitifolia, Kozgar et al 45 in Cicer arietinum, Khursheed and Khan 46 in Vicia faba and Laskar and Khan 17 in Lens culinaris also noticed mutagen www.nature.com/scientificreports www.nature.com/scientificreports/ induced variations in the electrophoresis patterns of the seed storage proteins. The unique pattern of protein bands may also serve as markers for screening mutant lines.…”

Section: Seed Protein and Mineral Element Contentsmentioning

confidence: 99%

“…Increased NRA in the mutants may also be attributed to the increased protein content in seeds in the mutants isolated in present experimentation. Genetic and environment interactions also influence the total protein quantity in seeds 35 .…”

Section: Seed Protein and Mineral Element Contentsmentioning

confidence: 99%

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Characterization of Induced High Yielding Cowpea Mutant Lines Using Physiological, Biochemical and Molecular Markers

Raina

Laskar

Tantray

et al. 2020

Sci Rep

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cowpea, Vigna unguiculata (L.) Walp. is an important grain legume grown in the dry agro-ecologies of the tropics with considerably low yield due to lack of improved varieties, aggravated by prevalent narrow genetic base. thus, induced mutagenesis was employed using sodium azide and gamma rays to increase genetic variability in cowpea genotypes that resulted in isolation of eleven high yielding mutant lines at the M 4 generation from the genetic background of cowpea varieties Gomati VU-89 and Pusa-578. In order to analyze the induced genetic divergence among the mutant lines and parent genotypes, biochemical and molecular characterization was carried out with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), simple sequence repeat (SSR) and cAAt box derived polymorphism (cBDp) markers. Activity of nitrate reductase (nR) and content of chlorophyll, carotenoid, protein and mineral were found to be significantly high in the selected mutant lines compared to their respective parent genotypes. SDS-PAGE profile of seed proteins generated 54 and 28 polymorphic bands and a total polymorphism of 62.06 and 41.17% in Gomati VU-89 and Pusa-578, respectively. SSR primers amplified a total of 16 and 24 polymorphic bands with an average polymorphism of 20.69 and 50.74% in Gomati VU-89 and Pusa-578, respectively. CBDP markers, used for the first time in mutagenized population, generated 175 bands with 77 bands being polymorphic in Gomati VU-89 and 121 bands with 59 bands being polymorphic in Pusa-578. Physiological, biochemical and molecular profiling of the selected promising mutants lines showed that Gomati VU-89-G and Pusa-578-C are genetically most diverged high yielding genotypes with significant increase in protein and micronutrient content, therefore, could be recommended for further research considerations. thus, the favorable combination of genes induced in the novel cowpea mutants selected through the present study are valuable to correspond farmers requirements for new improved cultivars (direct or hybrids).Pulses are the chief components of the agricultural system, effectively boosting food and nutrition, revenue and environment across the globe and hence assumed ideal for acquiring food security in the developing world including India. Even though India is the leading producer of pulses with 26% of the total world production harvested at about 35% of the total world area, yet a prominent yield gap of 18% exists and one of the leading importer nation 1 . Cowpea [Vigna unguiculata (L.) Walp.] is a vital leguminous crop growing in the semi-arid tropics of Asia, Africa, Southern Europe, Southern United States, and Central and South America 2 . Cowpea leaves, green pods, and grains are excellent dietary source of protein, thus used as food and feed for healthy growth of both humans and livestock 3 . Cowpea is reportedly considered to be first originated and domesticated in Southern Africa, and later spread to east and West Africa and Asia 4 . Worldwide about 6.5 million metric tons of cowpea are produc...

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Section: Seed Protein and Mineral Element Contentsmentioning

confidence: 74%

Section: Seed Protein and Mineral Element Contentsmentioning

confidence: 99%

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Characterization of Induced High Yielding Cowpea Mutant Lines Using Physiological, Biochemical and Molecular Markers

Raina

Laskar

Tantray

et al. 2020

Sci Rep

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“…These proteins contain the genetic information needed to generate different morpho-physiological and anatomical traits in plants. Mutagenic prompted modifications in the electrophoretic pattern of seed protein have been recorded earlier by several workers in different crops, such as in Glycine max [39], Cicer arietinum [40], Vicia faba [35], Lens culinaris [29] and cowpea [36]. Based on the above results, it could be possible to evaluate genetic variability and to distinguish the genetic pattern of protein bands of high yielding mutants through SDS-PAGE analysis…”

Section: Proteomic Studies In High Yielding Mutant Linesmentioning

confidence: 66%

Physio-biochemical analysis and molecular characterization of induced lentil mutant lines

Shahwar

Ansari²,

Park³

2022

PLoS ONE

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Lens culinaris is a proteinaceous food crop that is consumed worldwide for protein requirements. Mutation breeding has been used to improve protein content, yield, and related traits, as well as to select highly desirable mutants that are economically significant. An investigation of genotypic variation in lentil germplasm was carried out using induced mutagenesis, with caffeine, ethyl methane sulfonate (EMS), lead nitrate, and cadmium nitrate as mutagens that resulted in 18 mutant lines in the M3 generation. For the present study, we analyzed the genetic diversity of lentil mutant lines using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and random amplified polymorphic DNA markers (RAPD). The heterozygosity of RAPD markers per primer ranged from 50.00–90.90% with an average of 71.04%. The genetic divergent analysis was performed using hierarchical clustering (UPGMA), exhibited that these mutant lines were classified mainly into five subpopulation or clusters. A close resemblance with highest genetic coefficient similarity (1.00) were observed between control and mutant H; between mutant M and E; between mutant Q and J2, while more divergent mutants were N2 with mutant B; and mutant R with mutant J1with least genetic coefficient similarity (0.22). Protein and mineral content (Fe, Zn and Cu) were increased significantly in some high yielding mutant lines concerning to the control plant, and showed polymorphic variations in polypeptide chains in terms of banding pattern. Stomatal morphology in high yielding mutants were perceived utilizing scanning electron microscopy (SEM), exhibiting variations in stomatal size, stomatal opening and number of stomata. The present study’s promising mutant lines’ biological, physiological, and molecular profiles provide a foundation for forthcoming preservation and consumption strategies to broaden the genetic diversity of the breeding population of lentil.

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14. Surface graph and densitometric SDS-PAGE studies in chickpea mutants

Cited by 2 publications

References 9 publications

Characterization of Induced High Yielding Cowpea Mutant Lines Using Physiological, Biochemical and Molecular Markers

Characterization of Induced High Yielding Cowpea Mutant Lines Using Physiological, Biochemical and Molecular Markers

Physio-biochemical analysis and molecular characterization of induced lentil mutant lines

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