[14] Purification of alpha toxin from clostridium perfringens: Phospholipase C

Jolivet-Reynaud, Colette; Moreau, Hervé; Alouf, Joseph E.

doi:10.1016/s0076-6879(88)65017-8

Cited by 10 publications

(4 citation statements)

References 11 publications

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“…These results are in agreement with the physico-chemical characteristics of the ␣-toxin (one small hydrophobic enzyme with 43 kDa and pI 5.4 [28]) and a mechanism involving exclusion volume effect, hydrophobic and electrostatic interactions. Apparently these mechanisms are the main phenomena that govern the ␣-toxin partition in PEG-phosphate ATPS.…”

Section: Second 2 4-1 Experimental Designsupporting

confidence: 88%

Aqueous two-phase systems extraction of α-toxin from Clostridium perfringens type A

Cavalcanti

Porto

Neto³

et al. 2006

Journal of Chromatography B

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Section: Second 2 4-1 Experimental Designsupporting

confidence: 88%

Aqueous two-phase systems extraction of α-toxin from Clostridium perfringens type A

Cavalcanti

Porto

Neto³

et al. 2006

Journal of Chromatography B

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“…Many attempts to purify toxins from Clostridium perfringens have been reported (Krug, Kent, 1984;Moreau, Jolivet-Reynaud, Alouf, 1986;Stephen, 1961;Ito, 1968;Smyth, Arbuthnott., 1974;Mitsui, Mitsui, Hase, 1973;Möllby, Wadström, 1973;Bird, Low, Stephen, 1974;Takahashi, Sugahara, Ohsaka, 1974;Yamakawa, Ohsaka, 1977;Jolivet-Reynaud, Moreau, Alouf, 1988) However the separation is rather difficult since the variety of toxins produced from this microorganism is high (12 toxins are known) (Hirata et al 1995). Generally, the main methods used for protein purification are carried out by chromatography as ion exchange, affinity, hydrophobic interaction, and gel filtration.…”

Section: Introductionmentioning

confidence: 99%

Large scale purification of Clostridium perfringens toxins: a review

Cavalcanti¹,

Porto²,

Porto³

et al. 2004

Rev. Bras. Cienc. Farm.

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Clostridium perfringens, a Gram-positive anaerobic bacterium, is widespread in the environment and commonly found in the intestines of animals, including humans. C. perfringens strains are classified into five toxinotypes (A, B, C, D and E) based on the production of four major toxins (α, β, ε, ι). However the toxins (theta, delta, lambda and enterotoxin) are also synthesized by C. perfringens strain. Many attempts to purify the toxins produced by C. perfringens have been proposed. In this review we discuss the purification methods used to isolate toxins from C. perfringens reported in last four decades.

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“…Alpha toxin is commonly produced by all five types and is a phospholipase C that can hydrolyze lecithin into phosphorylcholine and diglyceride and is believed to be a major factor responsible for the organism's tissue pathology (2,19,20). This is the predominant product of C. perfringens type A.…”

mentioning

confidence: 99%

Molecular typing and epidemiological survey of prevalence of Clostridium perfringens types by multiplex PCR

Yoo¹,

Lee²,

Park³

et al. 1997

J Clin Microbiol

185

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Clostridium perfringens has been classified into five toxigenic types (A through E) on the basis of its capability to produce major lethal toxins (alpha, beta, epsilon, and iota toxins). Seroneutralization with mice or guinea pigs has been used to type each toxin, but this conventional method has some disadvantages. Therefore, we used a molecular biological technique to type the bacterium in the present study. A multiplex PCR was developed for this purpose. This method has several advantages in comparison with seroneutralization with mice or guinea pigs. By this method, we also investigated the most prevalent type(s) of the organism in Korean calves, piglets, and chickens showing clinical symptoms such as diarrhea, enterotoxemia, and necrotic enteritis. Only type A was isolated from calves and chickens, while type C (2 of 14 isolates), in addition to type A, was isolated from piglets. These results suggested that seroneutralization could be replaced by our new method and that type A of C. perfringens is the most prevalent type in livestock in Korea.

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[14] Purification of alpha toxin from clostridium perfringens: Phospholipase C

Cited by 10 publications

References 11 publications

Aqueous two-phase systems extraction of α-toxin from Clostridium perfringens type A

Aqueous two-phase systems extraction of α-toxin from Clostridium perfringens type A

Large scale purification of Clostridium perfringens toxins: a review

Molecular typing and epidemiological survey of prevalence of Clostridium perfringens types by multiplex PCR

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