1261 GP73, a resident golgi membrane protein, appears in sera of patients with viral liver disease and hepatocellular cancer

Romano, Piero; Николаева, Ольга Викторовна; Steel, Laura F.; Fimmel, Claus J.; Marrero, Jorge; Evans, Mark C.; Communale, M; Lok, Asf; DiBisceglie, Adrian M.; Block, Timothy

doi:10.1016/s0270-9139(03)81299-5

Cited by 3 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During the early development of Xenopus , we demonstrated that golph2 is expressed after the mid-blastula transition, in the dorsal blastopore lip and the derivatives from mesoderm (notochord and head endomesoderm) at the gastrula stage. The Spemann Organizer region secretes a cocktail of BMP antagonists, and the GOLPH2 protein has been reported to be secreted [35] , [36] . However, protein sequence predictions have not indicated the presence of any cysteine residues or CR motifs, which are common in the BMP antagonists [37] , and the injection of golph2 mRNA into embryos does not induce the expansion of the dorsal organ (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Xenopus as a Model System for the Study of GOLPH2/GP73 Function: Xenopus golph2 Is Required for Pronephros Development

Wen

Gong

et al. 2012

PLoS ONE

View full text Add to dashboard Cite

GOLPH2 is a highly conserved protein. It is upregulated in a number of tumors and is being considered as an emerging biomarker for related diseases. However, the function of GOLPH2 remains unknown. The Xenopus model is used to study the function of human proteins. We describe the isolation and characterization of Xenopus golph2, which dimerizes and localizes to the Golgi in a manner similar to human GOLPH2. Xenopus golph2 is expressed in the pronephros during early development. The morpholino-mediated knockdown of golph2 results in edema formation. Additionally, Nephrin expression is enhanced in the glomus, and the expression of pronephric marker genes, such as atp1b1, ClC-K, NKCC2, and NBC1, is diminished in the tubules and duct. Expression patterns of the transcription factors WT1, Pax2, Pax8, Lim1, GATA3, and HNF1β are also examined in the golph2 knockdown embryos, the expression of WT1 is increased in the glomus and expanded laterally in the pronephric region. We conclude that the deletion of golph2 causes an increase in the expression of WT1, which may promote glomus formation and inhibit pronephric tubule differentiation.

show abstract

Section: Discussionmentioning

confidence: 99%

Xenopus as a Model System for the Study of GOLPH2/GP73 Function: Xenopus golph2 Is Required for Pronephros Development

Wen

Gong

et al. 2012

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Because its relative molecular mass in polyacrylamide gel electrophoresis is 7.3 × 10 4 , it is also called GP73 . At present, many studies have found that the GP73 protein is associated with liver diseases, especially with liver cancer diseases; therefore, it is expected to become a new serological tumour marker for early diagnosis of liver cancer, which has attracted widespread attention from research scholars.…”

Section: Introductionmentioning

confidence: 99%

Golgi protein 73 and its diagnostic value in liver diseases

et al. 2018

View full text Add to dashboard Cite

Golgi protein 73 (GP73, also referred to as Golph 2) with 400 amino acids is a 73 kDa transmembrane glycoprotein typically found in the cis-Golg complex. It is primarily expressed in epithelial cells, which has been found upregulated in hepatocytes in patients suffering from both viral and non-viral liver diseases. GP73 has drawn increasing attention for its potential application in the diagnosis of liver diseases such as hepatitis, liver cirrhosis and liver cancer. Herein, we reviewed the discovery history of GP73 and summarized studies by many groups around the world, aiming at understanding its structure, expression, function, detection methods and the relationship between GP73 and liver diseases in various settings.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

show abstract