Determination of Phenolic Acids and Quercetin in Brazilian Red Wines from Vale do São Francisco Region Using Liquid-Liquid Ultrasound-Assisted Extraction and HPLC-DAD-MS

Abstract

This work describes a method for the determination of gallic, caffeic and p-coumaric acids, as well as quercetin, in Brazilian red wines by employing reverse phase high performance liquid chromatography (RP HPLC) coupled to diode array and mass detectors. The method was performed using a sample volume of 10 µL. The limits of detections were 0.36, 0.27, 0.33 and 0.59 mg L -1 for gallic acid, caffeic acid, p-coumaric acid and quercetin, respectively. The precision, as estimated by the relative standard deviation, was between 1.0 and 2.0% for different concentrations of gallic acid, caffeic acid, p-coumaric acid and quercetin. The accuracy was evaluated by addition and recovery experiments, and the obtained values were between approximately 92 and 117% recovery. The method was then applied to the analysis of red wine samples that were collected from the São Francisco region, Bahia State, Brazil. The concentrations of analytes were determined in ten Brazilian wines and varied from 5.26 to 10.22 mg L -1 for quercetin, 6.65 to 43.92 mg L -1 for gallic acid, 3.58 to 7.83 mg L -1 for p-coumaric acid, and 3.59 to 10.5 mg L -1 for caffeic acid.

Rapid determination of flavonoids and phenolic acids in grape juices and wines by RP-HPLC/DAD: Method validation and characterization of commercial products of the new Brazilian varieties of grape

“…In recent years methods for rapid determination of phenolic compounds in grape juice and wine have been published. In most cases these compounds are analyzed by Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) using a RP-C18 column types Core-Shell or rapid resolution (RR) for separating compounds (Dias, David, & David, 2016;Fontana, Antoniolli, & Bottini, 2016;Manns & Mansfield, 2012). However, it was not found studies describing a methodology for determination of phenolic compounds in wines and grape juices using an RR type column RP-C18 (100 Â 4.6, 3.5 lm), being necessary to carry out work to implement this type of column for analysis in these matrices as its presents an option for quick analysis.…”

Developing and validation of an HPLC-DAD method for the determination of eight phenolic constituents in extract of different wine species

“…7,8 The same relationshsip was proposed for wine consumption by different researchers. [8][9][10][11][12][13] Different fruits and vegetables show antioxidant properties. 1,2,7,14 Among the natural antioxidants, red grape and its product wine have received much attention due to the high concentration and great variety of phenolic compunds.…”

“…19 The antioxidant potential of wine is closely related to its phenolic content, which may be affected by a number of factors, including grape variety, fermentation processes, vinification techniques, ageing, and geographic and environmental factors (soil type and climate). 20 According to the literature, there are different methods to determine the phenolic contents of the different wine samples such as high-performance liquid chromatographymass spectrometry (HPLC-MS) 3,8,10,[21][22][23] , HPLC-diode-array detector (HPLC-DAD) 5,9,11,12,[24][25][26][27] , gas chromatography (GC) 19 , capillary electrophoresis (CE) 28 , and spectrophotometric 4,14,29,30 and electrochemical methods. 9,31 These methods come with some advantages and disadvantages.…”

Analytical Method by HPLC-Dad Allows Quantification of Quercetin Marker in Standardized Extract of Anadenanthera Colubrina Var. Cebil

“…colubrina has a large amount and class of secondary metabolites: tannins; alkaloids; flavonoids such as quercitrine; isoquercetrine; apigenin; quercetin; and other phenolic compounds [29][30][31][32][33].…”

“…Until now, no method validated by HPLC-DAD for quercetin quantification of HAE of A. colubrina has been reported in the literature. Properly validated analytical methods allow reproducible qualitative and quantitative analyses and ensure consistency of the analytical quality of a medicinal product [29,38]. In this research, a methodology will be developed for extracting the biomarker quercetin in the phytocomplex of A. colubrina and an analytical method for the quantification of quercetin in that plant's extract will be validated, according to the ICH (International Conference on Harmonisation) Q2 (R1) guidelines for HPLC-DAD [39][40].…”