Seasonal Effects on HPLC-DAD-UV and UPLC-ESI-MS Fingerprints and Analgesic Activities of<i>Vernonia Condensata</i>Baker Extracts

Afonso, Sabrina; Matos, Aldair Calistro de; Marengo, Vitor Almeida; Moreira, Estefânia Gastaldello; Soares, Daniely X.; Koolen, Héctor H. F.; Scarmínio, Ieda Spacino

doi:10.5935/0103-5053.20140286

Cited by 2 publications

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“…Metabolic fingerprinting has become increasingly applied to the investigation of chromatographic and/or spectral patterns for natural products [1][2][3][4]. These patterns are then analyzed to assess the quality and authenticity of commercial natural products, perform taxonomic classifications and determine biomarkers [5].…”

Section: Introductionmentioning

confidence: 99%

Mixture Design PARAFAC HPLC-DAD Metabolomic Fingerprints of Fractionated Organic and Basic Extracts from Erythrina speciosa Andrews Leaves

et al. 2018

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HPLC-DAD analysis of statistical mixture design extracts of Erythrina speciosa Andrews leaves provided chromatographic and UV-visible profiles of their basic and organic fractions that were treated with the PARAFAC multivariate method. The design extracts provided greater varieties and amounts of metabolites than could be obtained by classical extraction methods. Fractionation provided more diverse fingerprint information than obtained previously from only the crude extract. The two largest chromatographic peaks, one with a 4.8 min elution time having an intense spectral band at 235 nm and the other a 5.8 min peak with an intense 238 nm band for the basic fraction were obtained with the ternary 1:1:1 ethanol-dichloromethane-hexane mixture. These can be assigned to diene-type and lactonic alkaloids. Peaks with the same retention times are also found in the organic fraction but are extracted with different mixtures and have distinct spectral behavior in the 235 nm region, probably being aromatic alkaloids. The above strategy permits a more unambiguous assignment of metabolic groups to specific chromatographic peaks. This can be expected to provide higher quality chromatographic fingerprints for natural products' chemistry.

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Section: Introductionmentioning

confidence: 99%

Mixture Design PARAFAC HPLC-DAD Metabolomic Fingerprints of Fractionated Organic and Basic Extracts from Erythrina speciosa Andrews Leaves

et al. 2018

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“…Chemical fingerprinting of the 11 A. arborescens extracts prepared was performed by Fourier transform infrared (FTIR) spectroscopy and high-performance liquid chromatography (HPLC) as described by Afonso et al (2015). For chromatographic analysis, 0.005 g of each crude extract was diluted in 10 mL extraction solvent and subjected to an ultrasound bath for 15 min.…”

Section: Phytochemical Analysis Of Extractsmentioning

confidence: 99%

Phytochemical analysis and acaricidal activity of Aloe arborescens Mill. extracts against Rhipicephalus (Boophilus) microplus

Matos

Ribeiro

Scarmínio

et al. 2017

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The indiscriminate use of chemical acaricides has allowed Rhipicephalus (Boophilus) microplus to develop resistance to several active principles. However, botanical extracts have been tested as an alternative method to control those ticks. This experiment studied the chemical fingerprint and acaricidal effect of fresh and dry Aloe arborescens Mill. extracts on R. (B.) microplus. The acaricidal activity of extracts was assessed using in vitro assays with engorged females, and phytochemical characterization was performed by infrared (IR) spectroscopy and high-performance liquid chromatography (HPLC). The results showed that fresh and dry A. arborescens extracts prepared with the solvents pure ethanol, ethanol-dichloromethane binary mixture, and ethanol-dichloromethane-acetone ternary mixture, contained water-soluble tannins and had a strong effect on the reproductive parameters of R. (B.) microplus demonstrated by a marked decreased in the number of eggs laid and in the larvae hatching rate (p < 0.05, p < 0.01, p < 0.001). R. (B.) microplus, pois observou-se queda acentuada no número de ovos postos e na taxa de eclosão das larvas (p < 0,05; p < 0,01; p < 0,001). Conclui-se que A. arborescens Mill possui componentes com efeito acaricida contra R. (B.) microplus e que a fitoterapia, com os extratos dessa planta, pode ser utilizada como método alternativo para o controle de R. (B.) microplus.

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Seasonal Effects on HPLC-DAD-UV and UPLC-ESI-MS Fingerprints and Analgesic Activities ofVernonia CondensataBaker Extracts

Cited by 2 publications

References 1 publication

Mixture Design PARAFAC HPLC-DAD Metabolomic Fingerprints of Fractionated Organic and Basic Extracts from Erythrina speciosa Andrews Leaves

Mixture Design PARAFAC HPLC-DAD Metabolomic Fingerprints of Fractionated Organic and Basic Extracts from Erythrina speciosa Andrews Leaves

Phytochemical analysis and acaricidal activity of Aloe arborescens Mill. extracts against Rhipicephalus (Boophilus) microplus

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