Identification of Lipid Components in the Abdominal Muscle of Fall-Caught<i>Crangon crangon</i>from a Coastal Area of the Baltic Sea

Mika, Adriana; Skorkowski, Edward F.; Stepnowski, Piotr; Gołębiowski, Marek

doi:10.5935/0103-5053.20130061

Cited by 3 publications

(3 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The use of THAP as a MALDI matrix combined with the addition of lithium was shown to be advantageous for the selective detection of most lipid classes from total lipid extracts and complex phospholipid mixtures. 32 The use of MALDI TOF MS for sphingolipid detection from total lipid extracts has received less attention when compared to electrospray ionization (ESI) MS. [43][44][45][46][47][48] Sphingolipid detection is complicated by the relative low abundance of sphingolipids especially in comparison to high-abundance, favorably ionized glycerophospholipids. 12,49 The few reports that have used MALDI for sphingolipid analysis have incorporated a chemical and/or enzymatic hydrolysis step prior to sample analysis.…”

Section: Discussionmentioning

confidence: 99%

Lithium Hydroxide Hydrolysis Combined with MALDI TOF Mass Spectrometry for Rapid Sphingolipid Detection

Tran

Wan

et al. 2020

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

Sphingolipids have diverse structural and bioactive functions that play important roles in many key biological processes. Factors such as low relative abundance, varied structures, and a dynamic concentration range provide a difficult analytical challenge for sphingolipid detection. To further improve mass-spectrometry-based sphingolipid analysis, lithium adduct consolidation was implemented to decrease spectral complexity and combine signal intensities, leading to increased specificity and sensitivity. We report the use of lithium hydroxide as a base in a routine hydrolysis

show abstract

Section: Discussionmentioning

confidence: 99%

Lithium Hydroxide Hydrolysis Combined with MALDI TOF Mass Spectrometry for Rapid Sphingolipid Detection

Tran

Wan

et al. 2020

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

show abstract

“…The PUFA content is correlating with the life cycle of C. crangon —during spring and summer the development and the growth of young larval stages occurs (Abad et al , 1995). Simultaneously, the lowest contents of energy elements and the lowest values of the energy are noted in the period November–March (Campos et al , 2009), which explains the low level or the lack of PUFA in autumn and winter (Mika et al , 2013 and Table 2). The next factor—habitat—is directly connected with the type of food taken up.…”

Section: Discussionmentioning

confidence: 99%

The lipid composition of the abdominal muscle of shrimpCrangon crangonfrom the Gulf of Gdansk in spring and winter periods

et al. 2013

Self Cite

View full text Add to dashboard Cite

The composition of fatty acids and sterols of Crangon crangon abdomen muscle was determined during two periods in the year 2010/2011. For determination of lipids classes, especially of fatty acids and sterols, high performance liquid chromatography with a laser light-scattering detector and gas chromatography–mass spectrometry were applied. Diversity and variety of saturated and unsaturated fatty acids was the highest during the spring period. Twenty-seven free fatty acids with from nine to 24 carbon atoms were determined in the spring periods. Among this fraction, 14 saturated and 13 unsaturated fatty acids (eight mono- and five polyunsaturated) were present. Only seven saturated and four unsaturated free fatty acids (14:2, 17:1, 16:1 and 18:1) were identified in December 2010. Arachidonic acid (20:4) and eicosapentaenoic acid (20:5) were detected in these two periods, and during the spring season an additional essential fatty acid—docosahexaenoic acid (22:6)—was present, which was not detected in the winter period. The number of identified sterols was correlated with phytoplankton, which was abundant during April 2011. Also, the sterol fraction in winter periods was very poor—only cholesterol was detected.

show abstract

“…Accordingly, lipase activity in E. superba was reported to be four-fold higher in abdominal muscle and to represent a 15-fold higher share of total esterase activity compared to the whole animal [40]. Furthermore, lipids are well-documented as important energy reserve for crustaceans [41,42], in particular during periods of starvation or adaptation [43,44]. Since important storage reserves are localised in heptopancrease and the muscles, it suggests itself that enzymes for their mobilisation are produced in the same tissues [45].…”

Section: Functional Categorisation Of Krill Compartment Proteomesmentioning

confidence: 99%

Transcriptome–proteome compendium of the Antarctic krill (Euphausia superba): Metabolic potential and repertoire of hydrolytic enzymes

et al. 2022

View full text Add to dashboard Cite

The Antarctic krill (Euphausia superba Dana) is a keystone species in the Southern Ocean that uses an arsenal of hydrolases for biomacromolecule decomposition to effectively digest its omnivorous diet. The present study builds on a hybrid-assembled transcriptome (13,671 ORFs) combined with comprehensive proteome profiling. The analysis of individual krill compartments allowed detection of significantly more different proteins compared to that of the entire animal (1464 vs. 294 proteins). The nearby krill sampling stations in the Bransfield Strait (Antarctic Peninsula) yielded rather uniform proteome datasets. Proteins related to energy production and lipid degradation were particularly abundant in the abdomen, agreeing with the high energy demand of muscle tissue. A total of 378 different biomacromolecule hydrolysing enzymes were detected, including 250 proteases, 99 CAZymes, 14 nucleases and 15 lipases. The large repertoire in proteases is in accord with the protein-rich diet affiliated with E. superba's omnivorous lifestyle and complex biology. The richness in chitin-degrading enzymes

show abstract

Identification of Lipid Components in the Abdominal Muscle of Fall-CaughtCrangon crangonfrom a Coastal Area of the Baltic Sea

Cited by 3 publications

References 2 publications

Lithium Hydroxide Hydrolysis Combined with MALDI TOF Mass Spectrometry for Rapid Sphingolipid Detection

Lithium Hydroxide Hydrolysis Combined with MALDI TOF Mass Spectrometry for Rapid Sphingolipid Detection

The lipid composition of the abdominal muscle of shrimpCrangon crangonfrom the Gulf of Gdansk in spring and winter periods

Transcriptome–proteome compendium of the Antarctic krill (Euphausia superba): Metabolic potential and repertoire of hydrolytic enzymes

Contact Info

Product

Resources

About