DETERMINATION OF FLAVONOIDS IN STAMEN, GYNOECIUM, AND PETALS OFMagnolia grandifloraL. AND THEIR ASSOCIATED ANTIOXIDANT AND HEPATOPROTECTION ACTIVITIES

Abstract

Chromatographic analysis of flavonoids in ethyl acetate fractions of the stamen, gynoecium, and petal of Magnolia grandiflora L. by HPLC-PDA-MS/MS-ESI in the negative ionization mode was performed in this study. The results revealed the presence of eight flavonoids: apigenin 8-C-glucoside, luteolin 8-C-glucoside, quercetin 3-O-rutinoside, quercetin 3-O-galactoside, quercetin, 3-O-glucoside, kaempferol 3-O-rutinoside, isorhamnetin 3-O-glucoside, and isorhamnetin. Their quantification revealed that luteolin 8-C-glucoside is the major flavonoid and that the total phenolic content is concentrated primarily in the stamen. The antioxidant and hepatoprotective effects of ethanolic extract of the flower organs were evaluated against hepatotoxicity induced by CCl 4 , compared with the effects of silymarin.

Flavonoid Components of Different Color Magnolia Flowers and Their Relationship to Cultivar Selections

“…F10 had quasi molecular ion m/z 463[M-H]and fragment ion m/z 301[M-162] -, and the retention time of F10 was the same as that of the reference substance, quercetin 3-O-glucoside. Therefore, F10 was speculated to be quercetin 3-O-glucoside (Qu3G) (Sokkar et al, 2014). F11 and F12 had fragment ion 285[Y 0 ]in the reversephase HPLC and the same glycosides.…”

“…, and the retention time of F10 was the same as that of the reference substance, kaempferol 3-O-rutinoside. It was therefore speculated that F12 was kaempferol 3-Orutinoside (Km3G) (Sokkar et al, 2014) (Table 3, Supplemental Fig. 2).…”

“…In previous research, only one type of anthocyanin, cyanidin 3-O-glucoside chloride, was identified in Magnolia sprengeri, which was found to be the main pigment in this purpleflowered phenotype (Shi et al, 2015(Shi et al, , 2014. Six types of flavonoids have been identified from the ethanol extracts of white-flowered Magnolia grandiflora petals, including apigenin 8-Cglucoside, luteolin 8-C-glucoside, quercetin 3-Orutinoside, quercetin 3-O-galactoside, quercetin 3-O-glucoside, and kaempferol 3-O-rutinoside, whereas the flavonoids in the stamens have been identified as isorhamnetin 3-O-glucoside and isorhamnetin (Sokkar et al, 2014). However, there is no systematic study on the flavonoid composition and color of Magnolia petals.…”

Myrciaria Glazioviana Leaves Anti-Inflammatory Antinociceptive And Hepatoprotection Potential and Chemical Profile by Mass Spectrometry

“…This result suggests a potential hepatoprotective of the MGD extract. The hepatoprotective activity is, in many studies, directly associated to the presence of flavonoids [19][20][21] and antioxidant activity [22,23], which has already been described in M. glazioviana [4]. Liver diseases represent a global health problem given the importance of liver in the homeostasis.…”

First record of Tetra magnolivora (Keifer, 1939) (Acari, Eriophyidae) in Brazil

“…Its hard an, heavy wood is used to make furniture and other uses (Coladonato, 1991) and the leaves, fruits, bark and wood have been used fos medicinal purposes (Enciclopedia of life, 2017). The floral essential ois extracts were described as active against human lung carcinoma and breast carcinoma cells (Farag & Al-Mahdy, 2013) and also tested as antioxidant and hepatoprotectane (Sokkar et al, 2014). Keifer (1939) described Phyllocoptes magnolivora (Acari, Eriophyidae) from M. grandiflora collected in San Jose, California, USA.…”