Production of lipases with<i>Aspergillus niger</i>and<i>Aspergillus fumigatus</i>through solid state fermentation: evaluation of substrate specificity and use in esterification and alcoholysis reactions.

Reinehr, Christian Oliveira; Rizzardi, Juliana; Silva, Marceli Fernandes; Oliveira, Débora de; Treichel, Helen; Colla, Luciane Maria

doi:10.5935/0100-4042.20140077

Cited by 9 publications

(8 citation statements)

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“…Depois de resfriado (45 -55 °C), o meio foi inoculado em superfície a partir de uma cepa repicada, incubada por 4 dias à temperatura de 30 °C até completa esporulação. [11][12][13] Os esporos foram recuperados da superfície do meio utilizando-se 10 mL de água destilada estéril, com a ajuda de uma espátula estéril para a realização da raspagem dos esporos. A suspensão obtida foi conservada a 4 °C por até 30 dias.…”

Section: Preparo Das Soluções De Esporos De Aspergillus Niger E Flavusunclassified

Identification and Saccharifying Activity of Wild Microbial Flora Used in the Manufacturing of Tiquira

Ribeiro¹,

Moreira²,

Monteiro³

et al. 2019

Rev. Virtual Quim.

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Tiquira is an alcoholic distillate handmade prepared from the simultaneous saccharification and fermentation of cassava. The saccharification and fermentation processes are carried out by wild microorganisms, randomly harvested, from the natural exposure of beijus to the environment. The growth of these microorganisms occurs for a period of 8 days, and starch hydrolysis and sugar fermentation are performed for the same period. As there are several strains collected, the quality of the distillate is compromised, resulting in defects in the composition of the drink and in the variable process yield. The objective of this work was to isolate, identify and select, among the native microorganisms, the best saccharifying and fermentative agents, enabling the production of inoculum to reduce the process time and to homogenize Tiquira's secondaries. Among the selected strains, it was possible to identify and characterize three species of filamentous fungi: Aspergillus niger, Aspergillus flavus and Rhizopus oryzae. For all of them, SDA (Saboroud Dextrose Agar) medium, at 30 °C, was the one that best allowed fungal growth. Regarding starch saccharification yield, R. oryzae achieved a conversion value of 78.0 % (30 °C, 5.5 x 107 spore content, pH 5 and 50 g/L soluble starch), whereas A. flavus, A. niger, and the mixture of all of them, had a conversion rate of 71.5; 57.2 and 48.0 %, respectively, which highlights that the use of autochthonous filamentous microorganisms is an option for the saccharification of starch samples.

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Section: Preparo Das Soluções De Esporos De Aspergillus Niger E Flavusunclassified

Identification and Saccharifying Activity of Wild Microbial Flora Used in the Manufacturing of Tiquira

Ribeiro¹,

Moreira²,

Monteiro³

et al. 2019

Rev. Virtual Quim.

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“…Lipases (EC 3.1.1.3) are enzymes that can catalyze partial hydrolysis reactions or total triglycerides decomposition into free fatty acids, mono-and diglycerides, and also acting in esterification, transesterification and interesterification in low water environments (Reinehr et al, 2014). Lipolytic enzymes have been explored and used for novel biotechnological applications such as detergent, food, pharmaceutical and cosmetics production, chemical synthesis and in waste treatment (Carvalho et al, 2003;Colla et al, 2010;Colla et al, 2012;Kumar et al, 2012).…”

Section: Brazilian Journal Of Chemical Engineeringmentioning

confidence: 99%

“…Penicillium sp. and Yarrowia lipolytica are fungi normally used for the production of biosurfactants and lipolytic enzymes (Fontes et al, 2008;Fukuoka et al, 2008;Castiglioni et al, 2009;Kannahi and Sherley 2012;Kumar et al, 2012;Bhardwaj et al, 2013;Santos et al, 2013;Reinehr et al, 2014). Aspergillus niger is a wellknown lipase producer with suitable properties for industrial applications (Mahadik et al, 2002;Basheer et al, 2011).…”

Section: Brazilian Journal Of Chemical Engineeringmentioning

confidence: 99%

SIMULTANEOUS PRODUCTION OF BIOSURFACTANTS AND LIPASES FROM Aspergillus niger AND OPTIMIZATION BY RESPONSE SURFACE METHODOLOGY AND DESIRABILITY FUNCTIONS

Sperb

Costa

Bertoli

et al. 2018

Braz. J. Chem. Eng.

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Microbial conversion for the synthesis of high added-value compounds, such as biosurfactants and lipases, is one of the most promising fields within the biotechnology industry, given its current development. In this study the simultaneous production of biosurfactants and lipases by Aspergillus niger in a submerged bioprocess was analyzed. Full Factorial Design (2 3) was conducted to assess the influence of the malt extract (g•L-1 ; 25, 50, 75) and soybean oil concentrations (% v/v; 0.0, 1.25, 2.5) and agitation rates (rpm; 0.0, 100, 200) on biosurfactant and lipase production. Higher levels of the factors favored microbial growth, while their lower levels favored the production of biosurfactants and lipases. The variables were optimized through the response surface methodology and desirability functions, obtaining simultaneous maximized values of 0.48 g•L-1 (biomass); 42.03% (EA w/o);2.17 UE (EA w/o) and 3.28 U (LA). This indicates the possibility of combined biosurfactant and lipase production.

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“…19,20 Um grande número de métodos foi estabelecido para determinar a atividade lipolítica de um determinado extrato enzimático, compreendendo principalmente três tipos de ensaio: (a) quantificação dos ácidos graxos liberados por métodos titulométricos; (b) detecção de alterações por métodos condutométricos, turbidimétricos, densiométricos ou microscópicos das propriedades biofísicas dos substratos durante a lipólise; e (c) aplicação de métodos fotométricos para determinação de cor liberada de produtos fluorescents. 21 Em geral, os substratos mais comuns estudados são o azeite de oliva e o óleo de soja. 22 O método titulométrico de atividade enzimática tem sido utilizado para Rhizopus Chinensis, Apergillus niger, Cândida viscosum, Pseudomonas fluorescens, Rhizopus miehei e Rhizopus sp.…”

Section: Introductionunclassified

Chemical and Physicochemical Characterization of Faveleria (Cnidoscolus phyllacanthus) Oil - It Use of Determination of Enzymatic Activity of Extract of Fungi rhizopus sp.

Moura¹,

Neres²,

Moura³

et al. 2020

Rev. Virtual Quim.

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Production of lipases withAspergillus nigerandAspergillus fumigatusthrough solid state fermentation: evaluation of substrate specificity and use in esterification and alcoholysis reactions.

Cited by 9 publications

References 0 publications

Identification and Saccharifying Activity of Wild Microbial Flora Used in the Manufacturing of Tiquira

Identification and Saccharifying Activity of Wild Microbial Flora Used in the Manufacturing of Tiquira

SIMULTANEOUS PRODUCTION OF BIOSURFACTANTS AND LIPASES FROM Aspergillus niger AND OPTIMIZATION BY RESPONSE SURFACE METHODOLOGY AND DESIRABILITY FUNCTIONS

Chemical and Physicochemical Characterization of Faveleria (Cnidoscolus phyllacanthus) Oil - It Use of Determination of Enzymatic Activity of Extract of Fungi rhizopus sp.

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