SIMULTANEOUS PRODUCTION OF BIOSURFACTANTS AND LIPASES FROM Aspergillus niger AND OPTIMIZATION BY RESPONSE SURFACE METHODOLOGY AND DESIRABILITY FUNCTIONS

Abstract

Microbial conversion for the synthesis of high added-value compounds, such as biosurfactants and lipases, is one of the most promising fields within the biotechnology industry, given its current development. In this study the simultaneous production of biosurfactants and lipases by Aspergillus niger in a submerged bioprocess was analyzed. Full Factorial Design (2 3) was conducted to assess the influence of the malt extract (g•L-1 ; 25, 50, 75) and soybean oil concentrations (% v/v; 0.0, 1.25, 2.5) and agitation rates (rpm; 0.0, 100, 200) on biosurfactant and lipase production. Higher levels of the factors favored microbial growth, while their lower levels favored the production of biosurfactants and lipases. The variables were optimized through the response surface methodology and desirability functions, obtaining simultaneous maximized values of 0.48 g•L-1 (biomass); 42.03% (EA w/o);2.17 UE (EA w/o) and 3.28 U (LA). This indicates the possibility of combined biosurfactant and lipase production.

Improved biosurfactant production from Aspergillus niger through chemical mutagenesis: characterization and RSM optimization

“…Biosurfactant production from fungal species have extensively been described from Aspergillus spp. [41][42][43][44] using various synthetic and biobased substrates, but herein, mutant A. niger (M2) produce significantly higher amount of biosurfactant (3.3 g/L) as compared with previous studies using native A. niger strain. The mutant (A. niger M2) exhibited high emulsification index and oil displacement as compared to native strain (Table 1).…”

“…The biosurfactant production during logarithmic growth phase and stationary phase of microorganisms have already been studied from various fungal species e.g., A. ustus [42], A. niger [43,44], A. flavus [43,46], A. versicolor [47], and Piper hispidum [48], and various factors influencing the total biosurfactant yield during fermentation have broadly been studied in previous few decades [42,[49][50][51][52][53][54]. From these, several studies have reported classical methods for optimization purpose by placing one factor as constant and modifying another one.…”

Solid-state fermentation for low-cost production of biosurfactant by promising Mucor hiemalis UCP 1309

“…According to the literature, bacterial biosurfactants are more effective in reducing surface tension to values around 27-28 mN/m (Varjani, & Upasani, 2016;Liu, et al, 2018;Zhao, et al, 2020). However, in recent decades filamentous fungi have been explored in this sense, such as Aspergillus niger, Cunninghamella echinulata and Mucor circinelloides (Silva, et al, 2018;Sperb, et al, 2018;Marques, et al, 2019).…”

“…This means that an increase in WSO concentration led to lower surface tension, suggesting the production of biosurfactant in the culture medium. Previously, several studies demonstrated the effectiveness of hydrophobic substrates such as vegetable and waste frying oils as inductors for microbial biosurfactant production (Montero-Rodríguez, et al, 2015;Sperb, et al, 2018;Araújo, et al, 2019).…”

Bioprocess Optimization of Nutritional Parameters for Enhanced Anti-leukemic L-Asparaginase Production by Aspergillus candidus UCCM 00117: A Sequential Statistical Approach

“…To achieve this therefore, a medium formulated to maximize L-asparaginase activity while minimizing biomass concentration and total protein would be ideal. The desirability function is a mathematical protocol that recommends a set of operational conditions that meet all set goals with the best desirability (Sperb et al 2018 ). The composite desirability of a multi-response function was defined as the geometric mean of the desirability of individual responses and is given by the expression; …”