Fibronectina tópica no tratamento de úlcera de córnea de coelho após queimadura por álcali.

Abstract

Flgura l -Esquema gerai da moIéculade fibroneclina com seus domínios. G/y-Arg-G/y-Asp-Seréa seqüão cia de pentapeptídeos presente no domínio celular responsável pela ligação ao receptor celular.

Effects of a 5% monosulfated glycosaminoglycan and duasorb topic solution on alkali-burned rabbit corneas

“…Light microscopy showed PMN infiltration in the intact conjunctiva. The number of these cells was highest on the 10 th day, with the beginning of migration to the middle stroma, as previously shown by others (Hughes, 1946;Pfister and Burstein, 1976;Pfister, 1983;Burns et al, 1990). These cells are known to be the source of collagenolytic enzymes (Brown et al, 1970;Henriquez et al, 1976;Pfister and Burstein, 1976;Burns et al, 1989;Burns et al, 1990), which cause recurrence and, in some cases, perpetuation of the ulcers.…”

“…Sodium hydroxide, 3 M, caused corneal opacification of a porcelain-white color immediately after application to the cornea (Hughes, 1946). Cell lysis accompanied by collagen denaturation and hydrolysis of stromal glycosaminoglycans caused by the alkali (Pfister, 1983;Christmas, 1991) occurs by saponification of cell membrane lipids by hydroxyl through the reaction of the cation with the carboxylic groups of the glycosaminoglycans and collagen. These are the major elements involved in the pathogenesis of the lesions produced by alkaline substances (Wagoner, 1997).…”

“…The usual presence of alkalis in cleaning products and in construction materials contributes to the relatively frequent occurrence of chemical burns (Donzis and Mondino, 1987;Wagoner, 1997). Alkalis can rapidly penetrate the eye (Hughes, 1946;Pfister, 1983;Chung And Fagerholm, 1987;Christmas, 1991), lysing cells, denaturing collagen and hydrolyzing glycosaminoglycans of the stroma (Pfister, 1983;Christmas, 1991). Residues of the substance continue the destructive process unless they are completely removed (Hughes, 1946;Christmas, 1991).…”

“…Depending on the extent of penetration, alkalis can produce ischemia and necrosis of the sclera and conjunctiva, ulcers and perforations. They may also damage lacrimal glands and ducts, predisposing to temporary or permanent keratoconjunctivitis sicca (Christmas, 1991), and the episclera, the iris, the ciliary body, trabecular network and lens epithelium (Wagoner, 1997), with consequent uveitis, glaucoma and cataract (Hughes, 1946;Stein et al, 1973;Pfister, 1983;Christmas, 1991).…”

“…Among the clinical procedures proposed thus far are copious irrigation with water immediately after the accident and instillation of atropine for the relief of ciliary spasm and for the prophylaxis of synechiae (Hughes, 1946;Donzis and Mondino, 1987;Christmas, 1991). Other substances used are acetylcysteine (Burns et al, 1989), cysteine, sodium citrate and ascorbate (Donzis and Mondino, 1987;Burns et al, 1990;Christmas, 1991;Wagoner, 1997), bmercaptometthyl tripeptide, systemic tetracycline (BURNS et al, 1990), sodium EDTA, EDTA with calcium, blood serum (Rebhun, 1981, tear substitutes, epidermal growth factor, retinoic acid, sodium hyaluronate (Wagoner, 1997), and fibronectin (Burns et al, 1990;Mori et al, 1993), in addition to systemic or topical anti-prostaglandin drugs (Christmas, 1991;Wagoner, 1997).…”