Effect of the addition of the antioxidant taurine on the complete blood count of whole blood stored at room temperature and at 4°C for up to 7 days

Abstract

BackgroundThe complete blood count is one of the most common routine tests. This study aimed to evaluate possible effects of the antioxidant taurine on the complete blood count of whole blood stored at room temperature and at 4ºC over seven days. MethodsVenous blood samples of 25 healthy males were distributed into two sets of tubes with each set of four tubes containing 50 µL of solutions with zero, 2.5 g/L, 5 g/L, 10 g/L taurine. The tubes were kept at room temperature or at 4ºC. Complete blood counts were performed on seven successive days. The mean percentage changes [Δ = (mean value - mean baseline value) / mean baseline value x 100] were calculated and compared. ResultsComplete blood count parameters exhibited different patterns of behavior which were affected by the storage temperature, time and taurine concentration. Taurine at room temperature significantly enhanced the stability of: the platelet count over seven days (Δ7 at 2.5, 5 and 10 g/L taurine were 5.45, 6.11, and 5.80 x 109 cells/L, respectively); the red blood cell count over five days (Δ5 at 2.5, 5 and 10 g/L taurine were 1.59, 2.79, and 1.98 x 1012 cells/L, respectively); mean corpuscular hemoglobin over five days (Δ5 at 2.5, 5 and 10 g/L taurine were -0.91,-1.52 and -0.84 fl respectively); and red cell distribution width over two days (Δ2 at 2.5, 5 and 10 g/L taurine were 0.90%, 1.30% and -0.1%, respectively). No additional stabilizing effects of taurine were reported for the mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, hematocrit and hemoglobin, while it negatively affected the white blood cell stability. ConclusionComplete blood count parameters exhibited variable stability patterns in respect to temperature, time and taurine concentration.

Effects of exposure to electromagnetic field from 915 MHz radiofrequency identification system on circulating blood cells in the healthy adult rat

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Determining the stability of complete blood count parameters in stored blood samples using the SYSMEX XE‐5000 automated haematology analyser

“…Centralized haematology laboratories in the local health district hospitals in the era of new public health initiatives have adopted a single platform approach in providing standard services . Central laboratories provide the back up support to the network laboratories and now test specimens that have been dispatched over long distance; as a result, testing is often delayed by hours . When such specimens arrive after significant delay and under poor storage conditions, the results obtained from these specimens may lead to erroneous results without comments appended to the reported results regarding the reliability or limitations of analysis .…”

Cirrhosis-induced oxidative stress in erythrocytes: The therapeutic potential of taurine

“…The oxidation of fatty acids ultimately compromises the stability of the erythrocytes' membrane and eventually impairs their activity [152][153][154]. Interestingly, TAU is an excellent membrane stabilizer [130,149,[155][156][157]. It has repeatedly been mentioned that TAU protects biomembrane lipids from oxidation and consequently prevents cell damage [130,149,[155][156][157].…”

“…Interestingly, TAU is an excellent membrane stabilizer [130,149,[155][156][157]. It has repeatedly been mentioned that TAU protects biomembrane lipids from oxidation and consequently prevents cell damage [130,149,[155][156][157]. Huxtable et al mentioned that inhibiting lipid peroxidation, stabilization of biological membranes, TAU antioxidant properties, and maintaining intracellular ions' homeostasis play a crucial role in preserving cellular integrity and preventing their damage [23].…”