Determining the stability of complete blood count parameters in stored blood samples using the SYSMEX XE‐5000 automated haematology analyser

Abstract

The data presented suggest that clinically reliable results for some CBC parameters can be obtained from specimens that are stored at 4 °C for up to 72 h.

Evaluation and Comparison of Stability and Reliability of CBC Parameters Determined by Using Automatic Celltac G MEK-9100 Hematology Analyzer during Extended Storage at 4°C

“…Our result of HCT was stable up to 54 h that is similar to other studies at 4°C [6,15,17,19,20]. Result of MCV was found to be stable up to 42 h, regarding result for MCV at 4°C, previous studies are also show supporting with our findings [5,6,15,20,21].…”

“…Result of MCV was found to be stable up to 42 h, regarding result for MCV at 4°C, previous studies are also show supporting with our findings [5,6,15,20,21]. Studies by Turhan et al [5] and Lippi et al [2] demonstrated increment was observed on the second day due to reflect the swelling of RBC at room temperature.…”

“…Studies of Baca et al [21] Muller et al [23] and A. Joshi et al [15] show a stability time of 48 h to 72 h which is not much different from our findings. Demonstrated by Hedberg et al [20] and Muller et al [23] slight decrease in neutrophils at 4°C during 48 h to 72 h is identical to our result.…”

“…Storage condition and temperature: In this study, the selected temperature was 4 ± 1°C for storage, which is ideal for storage by WADA current guidelines [14,15]. Blood stored at <2°C can cause freezing injury to RBCs leading to homolysis while storage at >6°C can cause overgrowth of unspecified bacteria during blood sampling [10,12].…”

“…Blood stored at <2°C can cause freezing injury to RBCs leading to homolysis while storage at >6°C can cause overgrowth of unspecified bacteria during blood sampling [10,12]. Laboratory testing: The baseline measurement (before 20 min) were carried out after collection of samples on automatic MEK-9100 (Nihon Kohden Corporation), which works laser scatter+flow cytometry technology (Dyna Helix flow technology) [7,14,15]. After that, they were stored in refrigerator at 4°C.…”