Stability of complete blood count parameters depends on the storage temperature, storage time, transport position and selected stability criterion

Kayadibi, Hüseyin; Acar, Ismail Aytac; Cam, Samet

doi:10.1080/00365513.2020.1783570

Cited by 13 publications

(9 citation statements)

References 11 publications

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“…On the other hand, WBC counts were seen to decrease and HCT to increase at 48 h if blood samples were stored at 23 °C (Figure 1B and Figure 2B). The decrease in WBC counts and the increase in HCT has also been reported in previous reports when blood samples were stored at RT (9,19). In agreement with other reports, in the current study, significant increases in MCV and gradual decreases in MCHC were observed at 48 h storage time in blood samples stored at 23 °C.…”

Section: Resultssupporting

confidence: 93%

“…In the present study, MPV was found to be significantly increased during 20-48 h at all three storage temperatures. This finding confirms most previous studies which explain this finding with the result of platelet swelling (10,17,19). Regarding PLT, there are some conflicting results which have pointed out significant changes (17) and no significant changes (10) as were observed in the present study during 48 h storage at 4 °C, 10 °C or 23 °C.…”

Section: Resultssupporting

confidence: 92%

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Stability of hematological analytes during 48 hours storage at three temperatures using Cell-Dyn hematology analyzer

Unalli¹,

Özarda²

2021

J Med Biochemistry

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Background: The complete blood count (CBC) with differential leukocyte count (DLC) is one of the most common tests requested by physicians. The results of this test are affected by storage temperature and time of incubation. This study was designed to evaluate the stability of hematologic parameters in blood specimens stored for 48 hours at three temperatures. Methods: K2 EDTA- blood was collected from 22 apparently healthy adults. The CBC was performed using Cell-Dyn 3700 hematology analyzer (Abbott Diagnostics, IL, USA) immediately; 0 Time Point and at 4, 8, 12, 16, 20, 24 and 48 h after storage at 4°C, 10°C or 23°C. Changes in values of CBC parameters from the 0 Time Point were determined and reported as % of the initial value. Statistical comparisons were made using the paired-t test and the repeated measures ANOVA. Results: RBC, PLT, Hb and MCH were found stable during 48 h storage at 4°C, 10°C or 23°C. HCT and MCV increased, while WBC decreased significantly at 48 h when stored at 23°C. LYM, NEU, EOS and BASO values showed significant differences after 12 h of storage at 23°C. WBC, HCT, MCV, MCHC, RDW, NEU, MONO, BASO and EOS were stable for 48 h if blood samples were stored at 4°C or 10°C. Conclusions: RBC, PLT, Hb and MCH are the only suitable parameters without refrigeration during 24 h storage. When CBC and DLC are performed, 4°C can be recommended as the most suitable storage temperature for 12 h storage.

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Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 92%

Stability of hematological analytes during 48 hours storage at three temperatures using Cell-Dyn hematology analyzer

Unalli¹,

Özarda²

2021

J Med Biochemistry

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“…CRP was measured using routine analysis methodology (turbidimetric method, Cobas Integra 400 Plus) [13]. Complete blood count parameters were obtained by fluorescence flow cytometry on a Sysmex XT 4000i automated hematology analyzer [14]. Blood cultures were conducted when considered necessary, in order to diagnose concurrent pneumococcal bacteremia.…”

Section: Methodsmentioning

confidence: 99%

Is Multidrug Resistance in Acute Otitis Media with <b><i>Streptococcus pneumoniae</i></b> Associated with a More Severe Disease?

Horhat

Mocanu

2021

Med Princ Pract

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Objectives: Streptococcus pneumoniae is the leading bacterial etiologic agent in acute otitis media (AOM), and it produces a more severe inflammatory response than other otopathogens. Additionally, the presence of multidrug-resistant (MDR) S. pneumoniae is an important issue in the management of AOM. The present pilot study aimed to ascertain whether MDR S. pneumoniae is associated with a higher inflammatory response and/or a more severe disease. Methods: This was a prospective, single-center study on nonpneumococcal conjugate vaccine-immunized pediatric patients with severe AOM. Demographic and clinical characteristics were recorded. Middle ear fluid was obtained and cultured for each patient; antibiotic-resistance profiling was tested for S. pneumoniae isolates. The C-reactive protein (CRP) level and complete blood count were determined. Patients with positive middle ear fluid culture for S. pneumoniae were divided into 2 groups according to antibiotic resistance profile: MDR and non-MDR. Results: MDR S. pneumoniae was identified in 15 (35.7%) of the 42 eligible patients. Children in this group had significantly higher CRP levels (72.23 ± 62.92 vs. 14.96 ± 15.57 mg/L, p < 0.001), higher absolute neutrophil count (8.46 ± 3.97 vs. 5.22 ± 4.5 × 10³/mm³, p = 0.004), higher percentage of neutrophils (52.85 ± 13.49% vs. 38.34 ± 16.16%, p = 0.004), and were more prone to develop acute mastoiditis (p = 0.01). Receiver operating characteristic analysis identified CRP as the best biomarker to discriminate between the 2 groups of patients (AUC = 0.891). Conclusion: MDR S. pneumoniae was associated with a more severe inflammatory response and a higher incidence of mastoiditis.

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“… 1 I would add that it is considered best practice to process and analyze blood specimens within 1 hour of phlebotomy, since delays as brief as one hour have been noted to result in bias in the result of several CBC-based analytes, including platelet parameters such as the MPV. 3 …”

Section: Dear Editormentioning

confidence: 99%

Mean Platelet Volume and Its Predictive Role in Chronic Heart Failure: Technical Observations [Letter]

Frater¹

2022

IJGM

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Stability of complete blood count parameters depends on the storage temperature, storage time, transport position and selected stability criterion

Cited by 13 publications

References 11 publications

Stability of hematological analytes during 48 hours storage at three temperatures using Cell-Dyn hematology analyzer

Stability of hematological analytes during 48 hours storage at three temperatures using Cell-Dyn hematology analyzer

Is Multidrug Resistance in Acute Otitis Media with <b><i>Streptococcus pneumoniae</i></b> Associated with a More Severe Disease?

Mean Platelet Volume and Its Predictive Role in Chronic Heart Failure: Technical Observations [Letter]

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