Micellar high performance liquid chromatographic determination of Itraconazole in bulk, pharmaceutical dosage forms and human plasma

Abstract

A micellar liquid chromatographic method was developed for determination of Itraconazole in bulk, dosage form and human plasma using µBondapack cyano column and a mobile phase consisting of 0.1 M sodium dodecyl sulphate, 20% 1-propanol, 0.3% triethylamine in 0.02 M orthophosphoric acid (pH = 3.5). The UV detection was achieved at 258 nm. Various chromatographic parameters were studied, e.g. types of columns, pH of mobile phase, concentration of sodium dodecyl sulphate, 1-propanol, triethylamine, etc .The method was linear over the concentration range 16.4-320.0 µg/mL with regression coefficient 0.999 and limit of detection 5.4 µg/mL in bulk. Inter and intra-day results showed %RSD < 1.49%. The result obtained by the proposed method was compared with that obtained by the reference HPLC technique. Furthermore, the proposed method was successfully applied as stabilityindicating method for determination of Itraconazole under different stressed conditions. The method showed good selectivity, repeatability, linearity and sensitivity according to the evaluation of the validation parameters.

Micellar high performance liquid chromatographic determination of flunixin meglumine in bulk, pharmaceutical dosage forms, bovine liver and kidney

“…Micellar mobile phases usually need less quantity of organic modifier and generate less amount of toxic waste in comparison to aqueous-organic solvents, so that they are less toxic, non-inflammable, biodegradable and relatively inexpensive [18]. MLC has proved to be a useful technique in the determination of diverse [19][20][21][22][23][24], including food samples [25][26][27][28].…”

Singlet versus Triplet Excited State Mediated Photoinduced Dehalogenation Reactions of Itraconazole in Acetonitrile and Aqueous Solutions

“…It has been reported that ITR can undergo a primary dehalogenation process under UV light irradiation to form a biologically reactive aryl radical that may induce phototoxicity or photoallergy side effects. Some basic photochemical properties of ITR have been investigated using UV spectrophotometric, high-performance liquid chromatography and/or chromatography methods, − and a photoreaction product analysis has also been done by Miranda and co-workers . However, there is no in-depth time-resolved spectroscopic investigation about the specific mechanism of its photoreaction.…”