Micellar high performance liquid chromatographic determination of flunixin meglumine in bulk, pharmaceutical dosage forms, bovine liver and kidney

Abstract

a b s t r a c tA simple, sensitive and rapid liquid chromatographic method was developed and validated for the analysis of flunixin meglumine (flunixin-M) in bulk, pharmaceutical dosage forms, bovine liver and kidney. Analytical separation was performed in less than 4 min using a C 18 column with UV detection at 284 nm. A micellar solution composed of 0.15 M sodium dodecyl sulphate, 8% n-butanol and 0.3% triethylamine in 0.02 M phosphoric acid buffered at pH 7.0 was used as the mobile phase. The method was fully validated in accordance with the International Conference on Harmonization (ICH) guidelines. The limit of detection and the limit of quantitation were 0.02 and 0.06 lg mL À1 , respectively. The recoveries obtained were in range of 95.58-106.94% for bovine liver and kidney. High extraction efficiency was obtained without matrix interference in the extraction process and in the subsequent chromatographic determination. The method showed good repeatability, linearity and sensitivity according to the evaluation of the validation parameters.

Development of IC-ELISA and immunochromatographic strip assay for the detection of flunixin meglumine in milk

“…Currently, FM detection methods include high performance liquid chromatography (Belal, Abd El-Razeq, Fouad, & Fouad, 2015) and chromatography (Howard et al, 2014;Lee & Maxwell, 2014). Even though these methods are highly accurate and sensitive, they are costly and require complex sample pretreatment steps and trained personnel.…”

Spectrophotometric Methods for Simultaneous Determination of Oxytetracycline HCl and Flunixin Meglumine in Their Veterinary Pharmaceutical Formulation

“…Literature survey represented that several methods have been reported for determination of OXY alone or in combination with other drugs. These include spectrophotometric method [ 7 ], HPLC [ 8 – 11 ], colorimetric [ 12 , 13 ], fluorometric [ 14 , 15 ], and electrochemical methods [ 16 ], while FLU was determined by spectrophotometric [ 17 ], HPLC [ 18 – 23 ], and voltammetric methods [ 24 , 25 ]. To the best of our knowledge, no method has been reported for the simultaneous determination of OXY and FLU in their pharmaceutical preparation.…”

Voltammetric Determination of Flunixin on Molecularly Imprinted Polypyrrole Modified Glassy Carbon Electrode

“…The dosing formulation of FXN was the second most frequent violative residue detected by the USDA-Food Safety Inspection Service (FSIS) in 2010 [ 12 ]. To protect consumers from health-threatening residues, several analytical methods have been used for analyzing FXN in biological matrices, such as voltammetry [ 13 ], high performance liquid chromatography [ 14 – 16 ], thin-layer chromatography [ 17 ], gas chromatography [ 18 – 22 ], and capillary electrophoresis [ 23 ]. The analytical methods for the determination of FXN in milk included high-performance liquid chromatography (HPLC) [ 24 ], liquid chromatography/mass spectrometry (LC/MS) [ 21 , 25 , 26 ], and gas chromatography/mass spectrometry (GC/MS) [ 21 ].…”