Effect of calcitonin on lipid peroxidation in ovariectomized rats

Abstract

KEYWORDSCalcitonin is one of the active substances which is recently used effectively in osteoporosis treatment. In literature there is no information whether calcitonin cause oxidative stress or not. In this study, subcutaneous calcitonin is applied to rats having experimental postmenopausal osteoporosis and the effects of calcitonin on lipid peroxidation and antioxidant system were investigated. Forty-five healthy adult female Swiss albino Wistar rats were used in this study. The rats were divided into three equal groups as control, ovariectomized (Ovx) and ovariectomized+calcitonin (Ovx-CAL). The rats in Ovx and Ovx-CAL were anaesthetized and underwent a bilateral ovariectomy via ventral incision. Ten weeks after ovariectomy, salmon calcitonin (2 IU/kg body weight) was administered via s.c. at a volume of 1 mL per week for 12 weeks to the Ovx-CAL group. At the end of the drug treatment, livers and kidneys of rats were removed and malondialdehyde (MDA) levels and catalase (CAT) activities were determined by biochemical analysis methods. Data were analyzed by one-way ANOVA followed by the post-hoc LSD multiple test. The liver and kidney MDA levels were increased whereas the activity of CAT enzyme was decreased as a result of ovariectomy compared to values in the control group. Similar results were observed in Ovx-CAL group; however, the decline in the CAT activity in the kidney was not significant. In conclusion, we may suggest that calcitonin treatment increases the oxidative stress in osteoporotic rats. OsteoporosisOxidative stress Antioxidant Ovariectomy Calcitonin

Effect of Postmenopausal Strontium Ranelate Treatment on Oxidative Stress in Rat Skin Tissue

“…We also think that risendronate administration results in a statistically significant decrease in the level of MDA, which is due to the fact that this drug treats osteoporosis without triggering the oxidative stress. In a study conducted by Berkoz et al (22), it was found that administration of calcitonin to oophorectomized rats increased MDA levels in liver and kidney tissues and decreased liver catalase activity. In our study, we observed that strontium ranelate administration as a prophylactic (group IV) or therapeutic (group V) increased MDA levels in rat skin tissue, increased GSH level, SOD and catalase activities in oophorectomized rats compared to the control group.…”

“…Our results showed that administration of prophylactic or therapeutic strontium ranelate application to the oophorectomized rats increased MDA levels, decreased SOD and catalase activities, and did not alter GSH levels compared to the oophorectomized rats in the skin tissue. In literature, Berköz et al (22) found that liver and kidney MDA levels increased and liver catalase activity decreased after the administration of calcitonin to oophorectomized rats. Yalin et al (21) observed that risendronate administration reduced liver MDA levels and increased catalase activity in oophorectomized rats, nevertheless alendronate administration increased MDA levels, SOD and catalase activities in liver tissue of the oophorectomized rats.…”

Effects of single or combined administration of salmon calcitonin and omega-3 fatty acids vs. diclofenac sodium in sodium monoiodoacetate-induced knee osteoarthritis in male Wistar rats

“…Sct powder was dissolved in 0.9% of sodium chloride to obtain the desired doses [16]. The solution was stored in a refrigerator at 2 °C-8 °C for the maintenance of the hormone viability.…”