Prevalence of ehrlichial infection among dogs and ticks in Northeastern Brazil

Souza, Bárbara Maria Paraná da Silva; Leal, Danielle Custódio; Barboza, Débora Cristina Portella Medina; Uzêda, Rosângela Soares; Alcântara, Adriano Costa de; Ferreira, Fernando; Labruna, Marcelo Bahia; Gondim, Luís Fernando Pita; Franke, Carlos Roberto

doi:10.4322/rbpv.01902004

Cited by 16 publications

(3 citation statements)

References 32 publications

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“…The results for the city of Lavras (MG) is within the range reported by other studies conducted in Brazil, which have shown that between 14% and 45% of the dogs that are attended in veterinary hospitals and clinics are seropositive (18) . The frequency of E. canis in the serum of dogs in Lavras (MG) was lower than the results observed by Silva et al (19) in Cuiaba (MT), 42.5% (108/254; IFAT ≥40), and by Souza et al (20) in Salvador (BA), 35.6% (168/472; IFAT ≥80). However, Saito et al (21) observed a prevalence of 4.8% (19/389; IFAT ≥80) in Rio Grande do Sul, which was low compared to that obtained in the present study.…”

Section: Resultscontrasting

confidence: 49%

HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

et al. 2017

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The aim of this study was to determine the prevalence of anti-Ehrlichia canis and anti-Babesia vogeli IgG antibodies in dogs and correlate this prevalence with risk factors to evaluate the relation of serological status to hematological findings. Blood samples of dogs attended from September 2011 to March 2012 at the veterinary hospital of the Federal University of Lavras, Brazil, were analyzed using an indirect fluorescence antibody test (IFAT). Of the total 160 dog serum samples, 23.7% (38 dogs; CI 95 17.7% -30.7%) were seropositive for E. canis, 40.0% (64 dogs; CI 95 40.0% -59.2%) for B. vogeli, and 5.6% (9 dogs) for both hemoparasites. None of the epidemiological variables showed a significant association (P>0.05) with seropositivity to E. canis and B. vogeli. Dogs seropositive for E. canis showed lower values for hematocrit (P<0.05). However, for the erythrogram, the platelet count, the leukogram and clinical signs, no significant difference (P>0.05) was observed between dogs that were seropositive and seronegative for E. canis or for B. vogeli. Serological results suggest that infection with E. canis and B. vogeli is endemic in the canine population in question, with a prevalence of the subclinical phase (asymptomatic) in dogs that are seropositive for ehrlichiosis or babesiosis. Keywords: babesiosis; canine; ehrlichiosis; IFAT; laboratory findings. ResumoO objetivo deste estudo foi determinar a prevalência de anticorpos IgG anti-Ehrlichia canis e antiBabesia vogeli em cães e correlacionar com a prevalência e fatores de risco para avaliar a relação do estado sorológico com os achados hematológicos. Amostras de sangue de cães coletadas de setembro de 2011 a março de 2012, no Hospital Veterinário da Universidade Federal de Lavras, Brasil, foram analisadas usando o teste de reação de imunofluorescência indireta (RIFI). Do total de amostras de soro de 160 cães, 23,7% (38 cães; IC95 17,7% -30,7%) foram soropositivos para E. canis, 40,0% (64 cães; IC95 40,0% -59,2%) para B. vogeli, e 5,6% (9 cães) para os dois hemoparasitas. Nenhuma das variáveis epidemiológicas mostrou associação significativa (P> 0,05) com a soropositividade para E. canis e B. vogeli. Cães soropositivos para E. canis mostraram valores médios mais baixos para hematócrito (P<0,05). No entanto, para o eritrograma, a contagem de plaquetas, o leucograma e os sinais clínicos, nenhuma diferença significativa (P> 0,05) foi observada entre cães soropositivos e soronegativos para E. canis ou para B. vogeli. Os resultados sorológicos deste estudo sugerem que a Cienc. anim. bras., Goiânia, v.18, 1-9, e-36095, 2017 2 FONSECA, J.P., et al.infecção por E. canis e B. vogeli é endêmica na população canina em questão, com uma prevalência da fase subclínica (assintomática) em cães soropositivos para erliquiose ou babesiose.

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Section: Resultscontrasting

confidence: 49%

HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

et al. 2017

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“…A previous study in Southern Brazil using serological tests showed low occurrence of E. canis in asymptomatic dogs, of 4.8% (SAITO et al, 2008). However, higher prevalence was found in other Brazilian regions, ranging from 24.8% to 44.7%, using similar populations of dogs (AGUIAR et al, 2007;COSTA Jr et al, 2007aSOUZA et al, 2010). Although the tick vector R. sanguineus is abundant throughout all urban areas of Brazil (SAITO et al, 2008), including in the state of Rio Grande do Sul (RIBEIRO et al, 1997), it is important to consider that different populations of this tick species are found in Brazil (MORAES-FILHO et al, 2011).…”

Section: Discussionmentioning

confidence: 83%

Molecular detection of Ehrlichia canisand Anaplasma platys in dogs in Southern Brazil

Lasta

Santos

Messick

et al. 2013

Rev. Bras. Parasitol. Vet.

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The aims of this study were to determine the occurrence of Anaplasma platys and Ehrlichia canis infection in dogs in Porto Alegre, Southern Brazil; and to investigate their association with hematological abnormalities. Serum samples from 196 dogs were first tested using dot-ELISA for antibodies against Anaplasma spp. and Ehrlichia canis. Peripheral blood samples from 199 dogs were subjected to 16S rRNA nested PCR (nPCR) for A. platys and E. canis, followed by DNA sequencing to ensure pathogen identity. A total of 19/196 samples (9.69%) were positive for Anaplasma spp. using ELISA and 28/199 (14.07%) samples were positive for A. platys by nested PCR. All the dog samples were negative for E. canis, both in anti-E. canis antibody tests and in nested PCR. There were no significant differences in hematological parameters between A. platys-PCR positive and negative dogs and Anaplasma spp. serologically positive dogs, except for basophil counts, which were higher in nPCR-positive dogs. This is the first report showing A. platys presence in dogs in Southern Brazil. In conclusion, hematological parameters may not be sufficient to diagnose A. platys infection in dogs in Southern Brazil, probably due either to low pathogenicity or to chronic infection. On the other hand, E. canis may either have very low occurrence or be absent in dogs in Porto Alegre.

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“…antibodies in horses and human serum samples were evaluated by indirect immunofluorescent assay (IFA) using E. canis (São Paulo strain) and E. chaffeensis (Arkansas strain) as antigens. Crude antigens were produced by culturing ehrlichiae in DH82 cells, as previously described1 51. IFA was performed with 10 µ L of serum samples incubated at 37 °C for 30 min in slides previously seeded with E. canis or E. chaffeensis , washed three times for five min in phosphate buffered saline (PBS, pH 7.2), additionally washed by distilled water, then dried at room temperature.…”

Section: Methodsmentioning

confidence: 99%

SEROLOGICAL SURVEY OF Ehrlichia SPECIES IN DOGS, HORSES AND HUMANS: ZOONOTIC SCENERY IN A RURAL SETTLEMENT FROM SOUTHERN BRAZIL

Vieira

Nascimento

et al. 2013

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe aims of this study were to determine the seroprevalence of Ehrlichia spp. and risk factors for exposure in a restricted population of dogs, horses, and humans highly exposed to tick bites in a Brazilian rural settlement using a commercial ELISA rapid test and two indirect immunofluorescent assays (IFA) with E. canis and E. chaffeensis crude antigens. Serum samples from 132 dogs, 16 horses and 100 humans were used. Fifty-six out of 132 (42.4%) dogs were seropositive for E. canis. Dogs > one year were more likely to be seropositive for E. canis than dogs ≤ one year (p = 0.0051). Ten/16 (62.5%) and 8/16 (50%) horses were seropositive by the commercial ELISA and IFA, respectively. Five out of 100 (5%) humans were seropositive for E. canis and E. chaffeensis. Rhipicephalus sanguineus (n = 291, 97.98%) on dogs and Amblyomma cajennense (n = 25, 96.15%) on horses were the most common ticks found. In conclusion, anti-Ehrlichia spp. antibodies were found in horses; however, the lack of a molecular characterization precludes any conclusion regarding the agent involved. Additionally, the higher seroprevalence of E. canis in dogs and the evidence of anti-Ehrlichia spp. antibodies in humans suggest that human cases of ehrlichiosis in Brazil might be caused by E. canis, or other closely related species.

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Prevalence of ehrlichial infection among dogs and ticks in Northeastern Brazil

Cited by 16 publications

References 32 publications

HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

Molecular detection of Ehrlichia canisand Anaplasma platys in dogs in Southern Brazil

SEROLOGICAL SURVEY OF Ehrlichia SPECIES IN DOGS, HORSES AND HUMANS: ZOONOTIC SCENERY IN A RURAL SETTLEMENT FROM SOUTHERN BRAZIL

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