Análise da composição bioquímica da pele por espectroscopia Raman

Oliveira, Patrícia Karen; Tosato, Maira Gaspar; Alves, Rani de Souza; Martin, Aírton Abrahão; Fávero, P. P.; Raniero, Leandro

doi:10.4322/rbeb.2012.032

Cited by 12 publications

(8 citation statements)

References 21 publications

(44 reference statements)

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“…On the other hand, no statistical difference was observed between the irradiated and nonirradiated teeth for the amide I and CH 2 values, indicating that radiotherapy does not lead to the effective loss in quantity of the organic material . The characteristic vibrational modes of amide I are stretches between CO, with lower contribution of deformation of NH .…”

Section: Discussionmentioning

confidence: 86%

“…The characteristic vibrational modes of amide I are stretches between CO, with lower contribution of deformation of NH . The decrease in the amide peak I is related to damage or removal of the collagen fibers while CH 2 is related to the angular deformation of NH and bonding stretching CN, usually attributed to proteins, lipids, and carbohydrates …”

Section: Discussionmentioning

confidence: 99%

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Effect of radiotherapy on the chemical composition of root dentin

et al. 2018

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Background: The radiotherapy can directly affect the bond strength of the adhesive materials, interfering in the prognosis of restorative treatments, which may be caused by chemical changes in dentin structure. Methods: Twenty inferior homologues premolars were distributed in 2 groups (in vitro study) (n = 10): nonirradiated and irradiated. The specimens were submitted to the analysis of phosphate (ν 1 PO 4

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Effect of radiotherapy on the chemical composition of root dentin

et al. 2018

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“…Among these techniques, in vivo confocal Raman spectroscopy is a powerful method to identify and characterize changes in biochemical structures and molecular conformations resulting from aging processes in living tissues in real time (Oliveira et al, 2012;Tosato et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration

Teixeira

Rangel

Raniero

et al. 2014

RBEB

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Introduction: Skin health and skin care to reduce the effects of aging are the main interests of many researchers.The skin is very important because it protects the body from various effects of the external environment, and studies of the largest organ of the human body have been conducted since antiquity. In skin, aging effects are severe enough to promote changes in cell structure and biochemical composition. In this study, we quantitatively analyzed the water content and natural moisturizing factor of human facial skin in vivo and in real time by confocal Raman spectroscopy. This non-invasive technique is capable of providing detailed information on the biochemical composition at different depth profiles in the skin. Methods: We studied 10 volunteers, phototype II (40 and 50 years old), using a confocal Raman system to examine the skin surface down to 25 µm. Raman spectra were obtained before product use (T0), and after 30 days of continuous use of cosmetics (T30). Results:The results show a significant increase of 6.4% in water content in the surface layer of the facial skin after the cosmetic use. The amounts of natural moisturizing factor (NMF) compounds were also increased. Urocanic acid underwent a greater change in relation to carboxylic acid pyrrolidone, with a 38.5% increase in the stratum corneum. Conclusion: Confocal Raman spectroscopy identified changes in the biochemical composition of the superficial layers of the epidermis, which suggests the anti-aging efficacy of the formulation.

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“…The arrows in the figure help understand the trend of points, i.e., ratios higher than 1 indicate a relative increase in functional collagen, whereas a ratio less than 1 indicates an increase in procollagen. Therefore, the study of the process of wound healing in diabetes involves the understanding of the structures and biochemical components of collagen, a protein that plays an important role in biological tissues (Kim et al, 2000;Oliveira et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

FT-Raman spectroscopic study of skin wound healing in diabetic rats treated with Cenostigma macrophyllum Tul

Coelho

Raniero

Costa

et al. 2014

RBEB

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Introduction: Patients with diabetes mellitus exhibit a delay in the lesion repair process. The active components of Cenostigma macrophyllum may represent a viable alternative to facilitate the recovery of these lesions. The aim of this study was to evaluate the effects of emulsion oil-water Cenostigma macrophyllum in the repair process of lesions in rats with induced diabetes. Methods: 63 male rats (Wistar, 200-250 g body weight, 30-40 days old) were distributed into the following groups: control (C), diabetic (D) and diabetic treated with Cenostigma macrophyllum (P), subdivided based on the experimental times, days 7, 14 and 28, with 21 animals per main group. Diabetes mellitus (DM) was induced by administration of streptozotocin (50 mg/kg via penile vein and 12-h fasting) and confirmed at day 21 (glycemic index > 240 mg/dL). In the animals of group P, 0.5 ml of the oil-water emulsion obtained from the plant seed was used. The samples were removed and hemisectioned, and one portion was used for the quantitative histological analysis of collagen using Masson's trichrome staining, while another portion was analyzed by FT-Raman spectroscopy. Results: A higher percentage area of the volume of collagen fibers was observed for the experimental time Day 14 in group P compared with group D (p < 0.001). Regarding the ratio of areas of the amides I (1700-1600 cm -1 ) and III (1245-1345 cm -1 ), the groups D and P show the opposite behavior. Conclusion: Cenostigma macrophyllum accelerated the repair process in skin of diabetic ratsfor14 days.

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Análise da composição bioquímica da pele por espectroscopia Raman

Cited by 12 publications

References 21 publications

Effect of radiotherapy on the chemical composition of root dentin

Effect of radiotherapy on the chemical composition of root dentin

Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration

FT-Raman spectroscopic study of skin wound healing in diabetic rats treated with Cenostigma macrophyllum Tul

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