&lt;b&gt;Histochemical changes during the ontogeny of &lt;i&gt;malagueta&lt;/i&gt; and &lt;i&gt;biquinho&lt;/i&gt; pepper seeds

Abud, Haynna Fernandes; Araújo, Eduardo Fontes; Araújo, Roberto Fontes; Picoli, Edgard Augusto de Toledo; Gallão, Maria Izabel

doi:10.4025/actasciagron.v39i4.32492

Cited by 7 publications

(4 citation statements)

References 35 publications

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“…Important GAGs in the body are heparin, heparan sulfate, dermatan sulfate, hyaluronic acid, chondroitin sulfate, and keratan sulfate. As the sugar chain components of proteoglycans in the body, they are the main components of extracellular tissue matrix, cell surface, and cell membrane basal layer [12][13][14]. The histochemical staining of GAGs was less intense after implantation in this study.…”

Section: Discussionmentioning

confidence: 46%

The spatial and temporal distribution of cationic and anionic radicals in early embryo implantation

Bai

2020

Open Chemistry

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The main objective of this study is to obtain some knowledge of cationic and anionic radicals in early embryo implantation in mice. The method used in this study is known as histochemical staining, in which Xylidine ponceau was used at pH 2.5 and toluidine blue was used at pH 4.0. We detected the change in glycosaminoglycans and total proteins in the endometrial stroma during the preimplantation of mice. This study revealed that the distribution patterns of cationic radicals and anionic radicals are similar on days 4 and 5 of pregnancy. However, there was a distinct difference between cationic radicals and anionic radicals on day 8 of pregnancy. The distribution pattern of cationic radicals is more concentrated in the stroma near the conceptus. Laboratory studies on histochemical stain provide more information about early embryo implantation.

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Section: Discussionmentioning

confidence: 46%

The spatial and temporal distribution of cationic and anionic radicals in early embryo implantation

Bai

2020

Open Chemistry

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“…Regarding the morphological indicators, the best results (relative to the controls) were also achieved for seeds imbibed in the 6.25 totalU extract (Figure 1). Abud et al (2017) reported that proteins represent one of the main reserve components in Capsicum annuum L., seeds. These authors described how, in mature seeds, reserves are deposited both in the embryo and in the endosperm.…”

Section: Resultsmentioning

confidence: 99%

Bromelain crude extract to improve the emergence and growth of pepper seedlings

Gómez

Fernández

Báez

et al. 2022

rcia

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Pepper (Capsicum annuum L.) is one of the most consumed horticultural products worldwide. However, pepper seeds have slow and asynchronous germination. Organic agriculture encourages using natural substances for the pre-germinative treatment of seeds. The objective of the present work was to evaluate the effect of priming sweet pepper seeds with bromelain crude extract (obtained from pineapple stem residues) on subsequent seedling emergence and growth. The effects of the proteolytic activity of the extract (0, 3.16, 6.25, 12.25, and 25.0 totalU), imbibition time (0, 3, 6, 9, 12, and 15 h), and imbibition temperature (15, 25, 35 and 45 ℃) on seedling emergence and growth were assessed. The significantly best emergence, based on a range of indicators (emergence percentage: E, time to 50% emergence: TE50, emergence index: EI, mean emergence rate: MER, mean emergence time: MET, and synchronization index: SI), was obtained when the seeds were treated for three h at 35°C with a 6.25 totalU bromelain crude extract. Seeds subjected to this treatment combination also yielded the most vigorous seedlings (number of true leaves, length and thickness of the stems, fresh mass, dry mass, leaf area) . In conclusion, pre-germinative treatment with bromelain crude extract of 6.25 totalU proteolytic activity for three h at 35°C improved seedling emergence and growth in sweet pepper seedlings relative to untreated seed. This result offers an organic priming alternative for seeds of sweet pepper.

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“…The mobilization of reserves (proteins, carbohydrates and lipids) is crucial for germination e ciency and establishment of seedlings (Thompson 2018). Abud et al (2017) pointed out that proteins constitute one of the main reserve components in Capsicum annuum L. seeds. Galão et al (2007) observed in the seeds of Prosopis juli ora after 48 h under germination conditions, that the globular protein bodies were hydrolyzed and the protein material became more soluble.…”

Section: Resultsmentioning

confidence: 99%

Pineapple stem-derived bromelain based priming improves pepper seed protein reserve mobilization and germination

Perez¹,

Acosta²,

Nápoles³

et al. 2021

Preprint

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Pepper fruit has a great agronomic, nutritional and commercial value given that it is rich in antioxidant compounds such as carotenes, and vitamins C and E. However, pepper seeds are slow to germinate and emergence is often non-uniform and incomplete. Seed priming and invigoration treatments have been explored for a number of pepper varieties but success has been variable and generally limited. The present report describes the effects of pineapple stem – derived protease (stem bromelain) based priming on pepper seed germination in relation to reserve mobilization (specifically, proteins and aminoacids). Germination capacity in bromelain treated seeds was significantly higher on days 7, 14 and 21 than in unsoaked (control) and deionized water-soaked seeds but comparable across the treatments and the control on day 28. Germination rate was significantly highest in bromelain treated seeds. Light microscopy revealed an abundance of protein bodies in the endosperm of the seeds investigated at before imbibition and when observed over a period of 96 h, these bodies were progressively degraded, with the rate of this degradation being fastest in bromelain treated seeds. Quantitative measurements of protein levels (and free amino content) confirmed this observation. The results motivate the use of bromelain extracts for priming pepper seeds based on their proteolytic activity, since germination is dependent on the availability of crude protein and essential amino acids.

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<b>Histochemical changes during the ontogeny of <i>malagueta</i> and <i>biquinho</i> pepper seeds

Cited by 7 publications

References 35 publications

The spatial and temporal distribution of cationic and anionic radicals in early embryo implantation

The spatial and temporal distribution of cationic and anionic radicals in early embryo implantation

Bromelain crude extract to improve the emergence and growth of pepper seedlings

Pineapple stem-derived bromelain based priming improves pepper seed protein reserve mobilization and germination

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