&lt;b&gt;Micropropagation protocol for &lt;i&gt;Syngonanthus elegans&lt;/i&gt; (Bong.) Ruhland: an ornamental species

Pêgo, Rogério Gomes; Paiva, Patrícia Duarte de Oliveira; Paiva, Renato

doi:10.4025/actasciagron.v36i3.17946

Cited by 7 publications

(2 citation statements)

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“…Necrosis reduction could be explained by the amounts and sources of essential elements that are contributed by the WPM basal medium. MS and WPM mediums have almost the same content of calcium, around 3 mM in WPM, but MS supplies a higher amount of nitrogen, which also has been reported as one of the causes of STN (Pêgo et al, 2014). MS medium has four times more of NH +4 (20.61 vs 5.00 mM), and NO 3 -(39.41 vs 9.71 mM) (Silva et al, 2020).…”

Section: Discussionmentioning

confidence: 91%

Control of shoot-tip necrosis during Argylia radiata in vitro multiplication

Morales-Tapia

Gambardella

2022

Ornam. Hortic.

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Argylia radiata is an herbaceous perennial plant native to northern Chile and a representative species of the “Blooming Desert”. Due to its showy flowers and other morphological characteristics, A. radiata has great ornamental potential. In earlier work, a deep morpho-anatomical description was made, but the micropropagation protocols, which could be used for commercial purposes, are not known. Previous assays showed that cytokinin supplementation improves the multiplication rate but produces shoot-tip necrosis in the microplants. To avoid it, different modifications of the growth medium were tested, including calcium nitrate supplementation; increasing in agar concentration; indole-3-butyric acid enrichment; and change of the basal medium formulation. The effect of these changes over the damage level, number of shoots, multiplication rate, plant height (cm), fresh weight and dry weight (g), and water content (%) of the microplants were evaluated. The use of McCown Woody Plant formulation as basal medium showed the best effect, reducing the damage level and improving the multiplication rate. Additionally, IBA supplementation was effective in reducing necrotic damage. However, 0.1 mg L-1 of IBA significantly decreased the multiplication rate, while 0.01 mg L-1 led to a higher multiplication rate than that of plants grown in the control medium. In conclusion, the use of McCown Woody Plant medium and IBA supplementation should be considered in commercial A. radiata micropropagation.

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Section: Discussionmentioning

confidence: 91%

Control of shoot-tip necrosis during Argylia radiata in vitro multiplication

Morales-Tapia

Gambardella

2022

Ornam. Hortic.

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“…O desenvolvimento de técnicas de propagação para plantas nativas é importante para evitar o extrativismo insustentável e a extinção de espécies. Os conhecimentos obtidos por meio do estabelecimento de protocolos e metodologias específicos facilitam a domesticação, melhoramento e propagação de genótipos de interesse (PÊGO et al, 2014). Contudo, é possível otimizar a produção de mudas sadias em larga escala, com sanidade e identidade genética garantidas (WINHELMANN et al, 2019).…”

Section: Introdução Geralunclassified