Ex-vitro establishment of Phalaenopsis amabilis seedlings in different substrates

Venturieri, Giorgini Augusto; Arbieto, Elsa Aurora Mendoza de

doi:10.4025/actasciagron.v33i3.3950

Cited by 8 publications

(3 citation statements)

References 16 publications

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“…In addition, a low percentage of germination can be caused by immature embryos [19]. Based on a study [20], seeds from five month old V. tricolor fruits have the ability to develop into protocorms faster than seeds from seven month old fruits. Therefore, in this study seeds from fruit of V. tricolor which were four months old were used to see the viability and germination percentage of seeds ( Figure 3 and Table 1).…”

Section: Resultsmentioning

confidence: 99%

In vitroculture and characterization of theHSP70gene onVanda tricolorLindley var.Suavis‘Queen Maxima’

et al. 2020

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Vanda tricolor Lindey var. Suavis is an endemic orchid from Mt.Merapi, Yogyakarta Special Region, Indonesia. This orchid has beautiful flowers with unique patterns of white and purple spots, fragrant and can live in the slopes of Mt.Merapi which is a very active volcano. UGM in collaboration with the Netherlands carried out ex situ conservation of the V. tricolor Merapi through the self-pollination of orchids by the Queen of the Netherlands, Queen Maxima on March 11, 2020 at the UGM campus, Yogyakarta. In honor, the new generation of crosses is named Vanda tricolor var Suavis ‘Queen Maxima’. This study aims to produce mass quantities of the orchid and characterize the HSP70 gene in it. Methods: Standard in vitro culture for Vanda on MS, NP and VW medium, isolation of plant gDNA, PCR with V. tricolor HSP70primers and sequencing of amplicon DNA. The results showed that > 90 % of V. tricolor ‘Queen Maxima’ seeds germinated well in all media and the best on VW medium. V. tricolor has the HSP70 protein with PTZ00009 super family amino acid motif that 87 % similar to the HSP70 protein from the Phalaenopsis equestris orchid, which is probably the reason V. tricolor become superior to high temperature environments.

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Section: Resultsmentioning

confidence: 99%

In vitroculture and characterization of theHSP70gene onVanda tricolorLindley var.Suavis‘Queen Maxima’

et al. 2020

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“…Hal sebaliknya terjadi pada sphagnum, kemampuan menjerap air tinggi namun tingkat aerasi dan permeabilitas terhadap air rendah sehingga mengakibatkan pertumbuhan akar lebih rendah. Seidel Júnior dan Venturieri (2011), melaporkan terjadi penurunan kelangsungan hidup semai Cattleya forbesii dan Laelia purpurata dalam substrat sphagnum selama aklimatisasi.…”

Section: Hasil Dan Pembahasanunclassified

Naungan dan Tipe Substrat Berbeda pada Periode Aklimatisasi Ex-vitro Phalaenopsis Hibrid

Fauziah

Prahasti

Setiari

et al. 2020

BAF

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Produksi bibit Phalaenopsis Hibrid umumnya dilakukan melalui teknik kultur jaringan. Tahap selanjutnya adalah aklimatisasi plantlet di lingkungan ex-vitro. Penggunaan naungan dan tipe substrat berperan terhadap pertumbuhan dan kelangsungan hidup plantlet selama periode awal aklimatisasi. Penelitian ini mengkaji peran naungan dan tipe substrat yang berbeda terhadap pertumbuhan dan kelangsungan hidup plantlet Phalaenopsis Hibrid selama periode awal aklimatisasi di greenhouse. Penelitian menggunakan plantlet Phalaenopsis Hibrid dalam botol dan naungan paranet. Plantlet diberi 3 tipe substrat yaitu serabut kelapa, akar paku kadaka, sphagnum serta semua perlakuan dinaungi paranet secara bertahap. Parameter penelitian yang diukur meliputi: jumlah akar, total panjang akar, panjang daun, berat segar dan persentase kematian plantlet. Rancangan Acak Lengkap dengan satu faktor dan 3 ulangan digunakan dalam penelitian ini. Data dianalisis menggunakan ANOVA dan uji LSD pada taraf signifikansi 95% (P < 0,05). Penelitian dilakukan selama 5 minggu di greenhouse Jurusan Biologi, Fakultas Sains dan Matemetika, UNDIP. Plantlet tumbuh dan mampu bertahan hidup pada semua substrat dan naungan paranet secara bertahap. Pembentukan pori besar pada substrat serabut kelapa dan akar kadaka meningkatkan jumlah dan panjang akar. Kemampuan menjerap air tinggi pada spagnum meningkatkan berat segar plantlet. Penutupan paranet secara bertahap dan penggunaan substrat serabut kelapa, akar kadaka serta spagnum mempertahankan kelangsungan hidup plantlet selama periode awal aklimatisasi ex-vitro. Kata kunci:aklimatisasi, Phalaenopsis Hibrid, serabut kelapa, akar kadaka, sphagnum

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“…For orchids, different strategies were followed as previously described. [38][39][40][41][42] Pre-acclimatization of the in-vitro-grown plantlet was done in series of pre-acclimatization steps that were followed. The humidity inside the vessel of the in-vitro plantlets was reduced by making holes in the lid and left for 15 days.…”

Section: Hardening and Acclimatization Of Uncolonized And Colonized P...mentioning

confidence: 99%

Piriformospora indica promotes the growth of the in-vitro-raised Cymbidium aloifolium plantlet and their acclimatization

Shah

Thapa

Chand

et al. 2019

Plant Signaling & Behavior

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Cymbidium aloifolium is known for its ornamental and medicinal values. It has been listed as threatened orchid species. In this study, in vitro propagated C. aloifolium plantlets were interacted with the Piriformospora indica. The growth assay was performed for 45 days; the plant growth pattern such as number and length of roots and shoots were measured. Microscopic study of the root section stained by trypan blue was done to detect the peloton formation. The methanol extracts of the fungal colonized plant as well as uncolonized (control) plant were prepared and various metabolites were identified by gas chromatography mass spectroscopy. Acclimatization was done in a substrate composition of coco peat: gravel: charcoal in ratio 2:2:1. P. indica-colonized plantlet showed the highest growth with the formation of clamdospore in the root section. The growth regulator such as auxin, ascorbic acid, andrographolide, hexadecanoic acid, and DL-proline were identified. After three months of field transfer, plantlet colonized by P. indica survived and remained healthy as compared to uncolonized control plantlet.

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Ex-vitro establishment of Phalaenopsis amabilis seedlings in different substrates

Cited by 8 publications

References 16 publications

In vitroculture and characterization of theHSP70gene onVanda tricolorLindley var.Suavis‘Queen Maxima’

In vitroculture and characterization of theHSP70gene onVanda tricolorLindley var.Suavis‘Queen Maxima’

Naungan dan Tipe Substrat Berbeda pada Periode Aklimatisasi Ex-vitro Phalaenopsis Hibrid

Piriformospora indica promotes the growth of the in-vitro-raised Cymbidium aloifolium plantlet and their acclimatization

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