Piriformospora indica promotes the growth of the in-vitro-raised Cymbidium aloifolium plantlet and their acclimatization

Abstract

Cymbidium aloifolium is known for its ornamental and medicinal values. It has been listed as threatened orchid species. In this study, in vitro propagated C. aloifolium plantlets were interacted with the Piriformospora indica. The growth assay was performed for 45 days; the plant growth pattern such as number and length of roots and shoots were measured. Microscopic study of the root section stained by trypan blue was done to detect the peloton formation. The methanol extracts of the fungal colonized plant as well as uncolonized (control) plant were prepared and various metabolites were identified by gas chromatography mass spectroscopy. Acclimatization was done in a substrate composition of coco peat: gravel: charcoal in ratio 2:2:1. P. indica-colonized plantlet showed the highest growth with the formation of clamdospore in the root section. The growth regulator such as auxin, ascorbic acid, andrographolide, hexadecanoic acid, and DL-proline were identified. After three months of field transfer, plantlet colonized by P. indica survived and remained healthy as compared to uncolonized control plantlet.

Growth Evaluation of Banana cv. Barangan as the Effect of Trichoderma sp. and Covering Types during Acclimatization Process

“…There would be sudden changes of temperature, humidity and light conditions between in vitro and the field conditions. The sudden changes in environmental conditions promote abiotic and biotic stresses and even reduce the survival rate of the plantlets (Hazarika, 2003;Ortas et al, 2017;Wong et al, 2017;Shah et al, 2019). Furthermore, micro propagation of plants in the in vitro conditions may trigger the anatomy, physiology and morphology abnormalities of plantlets (Pospóšilová et al, 1999;Gutiarrez-Miceli et al, 2008;Chandra et al, 2010).…”

“…During acclimatization, several treatments are used in order to prepare better adaptation of the plantlets in the new environmental condition. Hardening process, medium composition and covering types are different among plant species and varieties (Gutiarrez-Miceli et al, 2008;Winarto and Prama Yufdy, 2017;Shah et al, 2019). Covering the plant is an important step to reduce water loss during this process.…”

“…Microbes play an important role in the acclimatization process of several plants (Pandey et al, 2000;Bag et al, 2001;Ortas et al, 2017;Shah et al, 2019). Dwarf Cavendish plantlets form mutualistic symbiosis with arbuscular mycorrhiza fungi to increase the plant growth and P-uptake (Ortas et al, 2017).…”

“…Dwarf Cavendish plantlets form mutualistic symbiosis with arbuscular mycorrhiza fungi to increase the plant growth and P-uptake (Ortas et al, 2017). Plantlets of Cymbidium aloifolium need to colonize with Piriformospora indica in order to increase their survival rate (Shah et al, 2019).…”

Asymbiotic Germination, Effect of Plant Growth Regulators, and Chitosan on the Mass Propagation of Stanhopea hernandezii (Orchidaceae)

“…The final step in a micropropagation protocol is the transfer of plant material from the in vitro culture to the ex vitro culture, the latter frequently considered as a bottleneck due to the high percentage of plants that are lost or damaged during acclimation to ex vitro conditions (Sherif et al 2018, Shah et al 2019. In vitro culture conditions generate plants with morphological, anatomical, and physiological alterations that make them susceptible to pathogen attack and render difficult their capacity to confront environmental conditions (Chandra et al 2010).…”