Germinação <em>in vitro</em> de grãos de pólen e efeito da proteção das plantas na frutificação de cubiu (<em>Solanum sessiliflorum</em> Dunal)

Crossing and diagnostic methods of cubiu hybrid plants based on genetic markers

“…One of them is the possibility of cultivating the plants without organza, since it is difficult to handle this fabric routinely. Moreover, the previous assumption in this study, that emasculation should be used to ensure the identity of the crossing is confirmed, despite the finding of Luz et al (2008), that the anthers are closed in the torpedo stage and that the in vitro germination of pollen grains is quite low at this time.…”

“…We tested five polymorphic primers for these cubiu varieties, previously identified by Luz et al (2008). The amplification reactions were repeated twice, testing 10 bulk samples of each assumed F 1 , including the parents.…”

“…In contrast, Storti (1988) characterized the cubiu plant as panmitic, owing to mechanisms that favor cross-pollination, such as the presence of poricidal anthers, resulting in low pollen fertility and JR Pizzinato et al asynchronous flowering. More recently, on 32 plants grown in a greenhouse covered with organza Luz et al (2008) found only one seedless fruit, which is therefore probably parthenocarpic, indicating cross-pollination of the species.…”