In vitro Propagation of Solanum sessiliflorum as Affected by Auxin and Cytokinin Combinations and Concentrations

Boufleuher, L. M.; Schuelter, Adílson Ricken; Luz, Cristiana Leonardi da; Antes, Vanessa Aparecida; Stefanello, Suzana; Comerlato, A. P.; Otoni, Wagner Campos

doi:10.3923/ajps.2008.639.646

Cited by 10 publications

(4 citation statements)

References 22 publications

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“…High sucrose concentrations create osmotic stress and reduce the absorption of elements and nutrients, which affects the multiplication of green parts. These results are consistent with previous studies, such as [18], which found that high sucrose concentrations reduced the multiplication of shoots in Solanum sessiliflorum compared to low concentrations. Similar results…”

Section: Effect Of Different Concentrations Of Ba In Single Node Mult...supporting

confidence: 93%

Role of Sucrose and Benzyladenine in Stevioside Content of Stevia Rebaudiana Leaves in Temporary Immersion Bioreactor

Alnuaimi,

Obaid

2023

IOP Conf. Ser.: Earth Environ. Sci.

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The experiment was carried out in the plant tissue culture laboratory of the Department of Horticulture and landscape gardening from October 2021 to January 2023. micropropagation experiments were conducted using concentrations of BA 0.0, 0.5, 1.0, 1.5 and 2.0 mg L, and an experiment with the addition of three levels of sugar (30, 60 and 90 g L) with two levels of BA (0.0 and 1.0 mg L). The results of the micropropagation showed that the concentration 1.0 mg L giving the highest average number of shoots of 3.5 shoots per plant, and giving the highest average number of leaves of 16.8 leaves of the shoot and the superiority of the comparison treatment in giving the highest average height of shoots amounting to 4.5 cm. The results showed that concentration 60 interacted with BA giving the highest average height of the shoots amounting to 4.3 plant shoots. and the concentration 60 interacted with the comparison treatment in giving the highest average shoot height of 4.4 cm and the concentration 90 interacted with BA in giving the highest average number of leaves amounting to 14.1 leaf shoot. The results indicated that the concentration 90 sucrose interacted with 1.0 mg L BA in giving the highest content of stevioside amounting 171.02

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Section: Effect Of Different Concentrations Of Ba In Single Node Mult...supporting

confidence: 93%

Role of Sucrose and Benzyladenine in Stevioside Content of Stevia Rebaudiana Leaves in Temporary Immersion Bioreactor

Alnuaimi,

Obaid

2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…These observations suggest that NAA in low concentrations triggers a positive effect in the growth of naranjilla plantlets from apical buds. However, this response could be species-dependent, as other studies report inhibitory effects of NAA alone or in combination with BAP for shoot development in Solanum sessiliflorum (Boufleuher et al 2008).…”

Section: Resultsmentioning

confidence: 99%

“…An alternative is the hybridization of naranjilla with interspecific members of the Lasiocarpa section (to which S. quitoense belongs), where resistance genes for different pathogens may exist (Heiser 1993). Nevertheless, these hybrids cannot be sexually propagated due to a progressive loss of the genotype of interest caused by gene-segregation in subsequent generations and sexual incompatibilities of genomes (Benítez et al 1991). One way to preserve and reproduce potentially interesting interspecific hybrids is vegetative propagation through in vitro culture.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Solanum quitoense var. quitoense by apical bud, petiole and hypocotyl culture

Gutiérrez

Cobo

Orellana

et al. 2019

Plant Biotechnology

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The development of in vitro propagation methods can improve the current commercial use and conservation of plants like naranjilla (Solanum quitoense), a distinctive Andean crop and key emerging agricultural product. In the present study, we report in vitro culture protocols for naranjilla apical buds, hypocotyls and petioles. In apical bud culture, MS medium supplemented with 0.10 mg l −1 1-naphtaleneacetic acid (NAA) produced longer plantlets with greater number of leaves. Hypocotyl culture yielded higher number of shoots when using older explants in MS medium supplemented with different combinations of NAA, 6-benzylaminopurine (BAP) and gibberellic acid (GA 3). Petiole culture produced a significantly higher number of shoots per explant, with more abundant and bigger leaves, when using MS medium supplemented with 0.02 mg l −1 NAA, 4.50 mg l −1 BAP and 1.00 mg l −1 GA 3. A factorial analysis reveals that the interaction between GA 3 and NAA/BAP plays an important role in shoot regeneration. These results provide new tools for the in vitro regeneration of naranjilla plants, improving on previously reported protocols for this species by using alternative explant types and regeneration protocols.

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“…In some cases, the hormonal combination of BAP-NAA has been shown to be harmful to the in vitro organogenesis of plant species. This is the case with Solanum sessiliflorum in which the combination of BAP (0.25 and 0.5 mg·L −1 ), and NAA (0.1 and 0.3 mg·L −1 ) did not have a beneficial effect on in vitro multiplication according to [43]. Thus, some authors recommend the use, instead of NAA, of other auxins such as IAA or IBA [44] [45].…”

Section: Micropropagationmentioning

confidence: 99%

In Vitro Clonal Propagation from Adult Material of a Savannah Species of Socio-Economic Importance: Annona senegalensis Pers.

Ba¹,

Dieme²,

Sy³

2021

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Annona senegalensis (Pers.) or Annone from Senegal is a species found in the West African savannah and para-littoral sands. It offers real possibilities of socio-economic use, particularly in the medical, nutritional, ecosystem conservation and poverty alleviation fields. However, this wild species remained not fully exploited despite its potentialities. As a result, there is a need to regenerate this species; therefore, the in vitro propagation from adult material was undertaken. For this purpose, axillary nodes from plant regrowth taken from elderly subjects were aseptically introduced into different media enriched or not with phytohormones. Among the 3 culture media tested, that of Murashige and Skoog (MS, 1962) was the most reactive and made it possible to develop a micropropagation protocol for adult material of A. senegalensis. Thus, the introduction of these explants in media containing cytokinins (BAP and Kinetin) significantly increased the reactivity compared to media without hormones. If the best average numbers of shoots (2.3) and nodes (5.3) are obtained in MS + BAP 2 mg·L −1 , with a reactivity of 91.66%, the addition of 0.1 mg·L −1 NAA gave the best average length (8.25 cm) of vitroplants. An induction time of 3 days into darkness with IBA at 50 mg·L −1 , followed by a transfer to hormone-free expression medium (MS/2) under light, resulted in a rooting rate of 58.33%. After the gradual weaning of the young plants in a mini-greenhouse, under shade for 3 weeks, 50% of the plants survived. This in vitro regeneration protocol can therefore be adopted for the clonal propagation of adult material of A. senegalensis. However, the rooting rate can be improved by trying other hormonal combinations based on auxins or by considering ex-vitro rooting during shade weaning.

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In vitro Propagation of Solanum sessiliflorum as Affected by Auxin and Cytokinin Combinations and Concentrations

Cited by 10 publications

References 22 publications

Role of Sucrose and Benzyladenine in Stevioside Content of Stevia Rebaudiana Leaves in Temporary Immersion Bioreactor

Role of Sucrose and Benzyladenine in Stevioside Content of Stevia Rebaudiana Leaves in Temporary Immersion Bioreactor

Micropropagation of <i>Solanum quitoense</i> var. <i>quitoense</i> by apical bud, petiole and hypocotyl culture

In <i>Vitro</i> Clonal Propagation from Adult Material of a Savannah Species of Socio-Economic Importance: <i>Annona senegalensis</i> Pers.

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In vitro Propagation of Solanum sessiliflorum as Affected by Auxin and Cytokinin Combinations and Concentrations

Cited by 10 publications

References 22 publications

Role of Sucrose and Benzyladenine in Stevioside Content of Stevia Rebaudiana Leaves in Temporary Immersion Bioreactor

Role of Sucrose and Benzyladenine in Stevioside Content of Stevia Rebaudiana Leaves in Temporary Immersion Bioreactor

Micropropagation of <i>Solanum quitoense</i> var. <i>quitoense</i> by apical bud, petiole and hypocotyl culture

In &lt;i&gt;Vitro&lt;/i&gt; Clonal Propagation from Adult Material of a Savannah Species of Socio-Economic Importance: &lt;i&gt;Annona senegalensis&lt;/i&gt; Pers.

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In <i>Vitro</i> Clonal Propagation from Adult Material of a Savannah Species of Socio-Economic Importance: <i>Annona senegalensis</i> Pers.